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Overview Data source: UniProt

Official Gene Symbol Other Aliases
C9 N/A
Sequence Length (AA) Molecular Weight (Da)
548 62002
Protein Name
Complement component C9
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASGMAITLA LAIFALGVNA QMPIPVSREE QEQHYPIPID CRMSPWSNWS
60 70 80 90 100
ECDPCLKQRF RSRSILAFGQ FNGKSCVDVL GDRQGCEPTQ ECEEIQENCG
110 120 130 140 150
NDFQCETGRC IKRRLLCNGD NDCGDYSDEN DCDDDPRTPC RDRVAEESEL
160 170 180 190 200
GLTAGYGINI LGMEPLRTPF DNEFYNGLCD RVRDEKTYYR KPWNVVSLIY
210 220 230 240 250
ETKADKSFRT ENYDEHLEVF KAINREKTSN FNADFALKFS ATEVPEKGAG
260 270 280 290 300
EVSPAEHSSK PTNISAKFKF SYFMGKNFRR LSSYFSQSKK MFVHLRGVVQ
310 320 330 340 350
LGRFVMRNRD VVLRSTFLDD VKALPTSYEK GEYFGFLETY GTHYSTSGSL
360 370 380 390 400
GGQYEIVYVL DKASMKEKGV DLNDVKHCLG FNMDLRIPLQ DDLKDASVTA
410 420 430 440 450
SVNADGCIKT DNGKTVNITR DNIIDDVISF IRGGTREQAI LLKEKILRGD
460 470 480 490 500
KTFDKTDFAN WASSLANAPA LISQRMSPIY NLIPLKIKDA YIKKQNLEKA
510 520 530 540 548
VEDYIDEFST KRCYPCLNGG TIILLDGQCL CSCPMMFRGM ACEIHQKI

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-1111 Collapse assay details

Data source: Panorama

Official Gene Symbol
C9
Peptide Sequence
LSPIYNLVPVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
473
Peptide End
483
CPTAC ID
non-CPTAC-1111
Peptide Molecular Mass
1,241.7384
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.2 5.9 6.1 7 5.8 5.7 8.7 8.3 8.3 7 15 15
y9 (2+) 2.7 4.2 3.5 8.8 4.9 3.3 9.2 6.5 4.8 15 15 15
y3 (1+) 6.3 5.4 2.9 11.2 6.4 3.2 12.9 8.4 4.3 15 15 15
y7 (1+) 6.1 5.2 6.2 10.2 5.4 7.8 11.9 7.5 10 15 15 15
y9 (1+) 6.3 4.4 5.3 12 4.7 4.9 13.6 6.4 7.2 15 15 15
sum 2.8 3.5 2.6 8.9 4.3 2.9 9.3 5.5 3.9 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-3779 Collapse assay details

Data source: Panorama

Official Gene Symbol
C9
Peptide Sequence
TENYDEHLEVFK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
210
Peptide End
221
CPTAC ID
non-CPTAC-3779
Peptide Molecular Mass
1,522.6940
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 3.2 4.4 4.1 4 8.9 5.8 5.1 9.9 7.1 15 15 15
y9 (2+) 20.8 8.5 6.7 21.6 9.6 7.8 30 12.8 10.3 15 15 15
y10 (2+) 6.7 6.2 4.5 7.8 9.6 7.2 10.3 11.4 8.5 15 15 15
y11 (2+) 17.8 7.3 5.6 16 9.2 8.5 23.9 11.7 10.2 15 15 15
y2 (1+) 14.2 3.8 5.4 28.4 7 9.3 31.8 8 10.8 15 15 15
sum 4.6 3.4 2.6 7 7.8 5.7 8.4 8.5 6.3 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2656 Collapse assay details

Data source: Panorama

Official Gene Symbol
C9
Peptide Sequence
TSNFNAAISLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
232
Peptide End
242
CPTAC ID
non-CPTAC-2656
Peptide Molecular Mass
1,164.6139
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 5.7 8.3 5.8 15.7 9.1 6.2 16.7 12.3 8.5 15 15 15
y9 (2+) 11.2 6.9 7.5 15.9 8.6 9.7 19.4 11 12.3 15 15 15
b3 (1+) 24 9.9 7.7 20.1 11.2 8.1 31.3 14.9 11.2 15 15 15
y7 (1+) 8.7 6.6 5.3 9.4 9.9 5.5 12.8 11.9 7.6 15 15 15
y5 (1+) 15.7 6.2 9.8 15.1 6.8 12 21.8 9.2 15.5 15 15 15
sum 4.8 3.6 4.3 9.9 6.6 4.9 11 7.5 6.5 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-1110 Collapse assay details

Data source: Panorama

Official Gene Symbol
C9
Peptide Sequence
VVEESELAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
146
Peptide End
154
CPTAC ID
non-CPTAC-1110
Peptide Molecular Mass
1,030.5295
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 18.3 14 6.6 23.9 12.1 9.4 30.1 18.5 11.5 13 15 15
y3 (1+) 21.6 9.8 13.3 14.7 8.8 11 26.1 13.2 17.3 8 15 15
y5 (1+) 28.4 6 5.4 38.3 9 7.5 47.7 10.8 9.2 8 15 15
y6 (1+) 8.2 13.2 8.3 12 12 9 14.5 17.8 12.2 11 15 15
y7 (1+) 10.8 7.3 8.7 11.6 9.6 9.3 15.8 12.1 12.7 15 15 15
sum 11.4 5.9 6 12.5 8.4 6.2 16.9 10.3 8.6 15 15 15

Additional Resources and Comments