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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Nap1l4

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Nap1l4 N/A
Sequence Length (AA) Molecular Weight (Da)
375 42679
Protein Name
Nucleosome assembly protein 1-like 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAENSLSDGG PADSVEAAKN ASNTEKLTDQ VMQNPQVLAA LQERLDNVSH
60 70 80 90 100
TPSSYIETLP KAVKRRINAL KQLQVRCAHI EAKFYEEVHD LERKYAALYQ
110 120 130 140 150
PLFDKRREFI TGDVEPTDAE SAWHSENEEE DKLAGDMKNK VVIAEKEAAT
160 170 180 190 200
VEELNPKGIP EFWFTIFRNV DMLSELVQEY DEPILKHLQD IKVKFSDPGQ
210 220 230 240 250
PMSFVLEFHF EPNDYFTNPV LTKTYKMKSE PDKADPFSFE GPEIVDCDGC
260 270 280 290 300
TIDWKKGKNV TVKTIKKKQK HKGRGTVRTI TKQVPNESFF NFFSPLKASG
310 320 330 340 350
DGESLDEDSE FTLASDFEIG HFFRERIVPR AVLYFTGEAI EDDDNFEEGE
360 370 375
EGEEEELEGD EEGEDEDDAD VNPKV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3850 Collapse assay details

Data source: Panorama

Official Gene Symbol
Nap1l4
Peptide Sequence
EAATVEELNPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
147
Peptide End
157
CPTAC ID
non-CPTAC-3850
Peptide Molecular Mass
1,199.6034
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 11.8 4.8 4.7 10.1 6.6 7.3 15.5 8.2 8.7 15 15 15
y6 (1+) 6.5 4.2 3.1 8 10 5.8 10.3 10.8 6.6 15 15 15
y4 (1+) 31.6 11.1 10.4 43.1 11.4 10.9 53.4 15.9 15.1 15 15 15
y2 (1+) 7.8 5.4 2.7 10.6 11.3 7.9 13.2 12.5 8.3 15 15 15
b2 (1+) 11.3 5.2 7 14.4 11.1 7.8 18.3 12.3 10.5 15 15 15
sum 4.2 1.8 1.6 6.9 8.8 5.2 8.1 9 5.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments