Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Notch2

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Notch2 N/A
Sequence Length (AA) Molecular Weight (Da)
2470 265327
Protein Name
Neurogenic locus notch homolog protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPDLRPAALR ALLWLWLCGA GPAHALQCRG GQEPCVNEGT CVTYHNGTGF
60 70 80 90 100
CRCPEGFLGE YCQHRDPCEK NRCQNGGTCV PQGMLGKATC RCAPGFTGED
110 120 130 140 150
CQYSTSHPCF VSRPCQNGGT CHMLSRDTYE CTCQVGFTGK QCQWTDACLS
160 170 180 190 200
HPCENGSTCT SVASQFSCKC PAGLTGQKCE ADINECDIPG RCQHGGTCLN
210 220 230 240 250
LPGSYRCQCG QGFTGQHCDS PYVRGLPCVN GGTCRQTGDF TLECNCLPGF
260 270 280 290 300
EGSTCERNID DCPNHKCQNG GVCVDGVNTY NCRCPPQWTG QFCTEDVDEC
310 320 330 340 350
LLQPNACQNG GTCTNRNGGY GCVCVNGWSG DDCSENIDDC AYASCTPGST
360 370 380 390 400
CIDRVASFSC LCPEGKAGLL CHLDDACISN PCHKGALCDT NPLNGQYICT
410 420 430 440 450
CPQGYKGADC TEDVDECAMA NSNPCEHAGK CVNTDGAFHC ECLKGYAGPR
460 470 480 490 500
CEMDINECHS DPCQNDATCL DKIGGFTCLC MPGFKGVHCE LEVNECQSNP
510 520 530 540 550
CVNNGQCVDK VNRFQCLCPP GFTGPVCQID IDDCSSTPCL NGAKCIDHPN
560 570 580 590 600
GYECQCATGF TGILCDENID NCDPDPCHHG QCQDGIDSYT CICNPGYMGA
610 620 630 640 650
ICSDQIDECY SSPCLNDGRC IDLVNGYQCN CQPGTSGLNC EINFDDCASN
660 670 680 690 700
PCMHGVCVDG INRYSCVCSP GFTGQRCNID IDECASNPCR KGATCINDVN
710 720 730 740 750
GFRCICPEGP HHPSCYSQVN ECLSNPCIHG NCTGGLSGYK CLCDAGWVGV
760 770 780 790 800
NCEVDKNECL SNPCQNGGTC NNLVNGYRCT CKKGFKGYNC QVNIDECASN
810 820 830 840 850
PCLNQGTCFD DVSGYTCHCM LPYTGKNCQT VLAPCSPNPC ENAAVCKEAP
860 870 880 890 900
NFESFSCLCA PGWQGKRCTV DVDECISKPC MNNGVCHNTQ GSYVCECPPG
910 920 930 940 950
FSGMDCEEDI NDCLANPCQN GGSCVDHVNT FSCQCHPGFI GDKCQTDMNE
960 970 980 990 1000
CLSEPCKNGG TCSDYVNSYT CTCPAGFHGV HCENNIDECT ESSCFNGGTC
1010 1020 1030 1040 1050
VDGINSFSCL CPVGFTGPFC LHDINECSSN PCLNAGTCVD GLGTYRCICP
1060 1070 1080 1090 1100
LGYTGKNCQT LVNLCSRSPC KNKGTCVQEK ARPHCLCPPG WDGAYCDVLN
1110 1120 1130 1140 1150
VSCKAAALQK GVPVEHLCQH SGICINAGNT HHCQCPLGYT GSYCEEQLDE
1160 1170 1180 1190 1200
CASNPCQHGA TCNDFIGGYR CECVPGYQGV NCEYEVDECQ NQPCQNGGTC
1210 1220 1230 1240 1250
IDLVNHFKCS CPPGTRGLLC EENIDECAGG PHCLNGGQCV DRIGGYTCRC
1260 1270 1280 1290 1300
LPGFAGERCE GDINECLSNP CSSEGSLDCV QLKNNYNCIC RSAFTGRHCE
1310 1320 1330 1340 1350
TFLDVCPQKP CLNGGTCAVA SNMPDGFICR CPPGFSGARL QSSCGQVKCR
1360 1370 1380 1390 1400
RGEQCIHTDS GPRCFCLNPK DCESGCASNP CQHGGTCYPQ RQPPHYSCRC
1410 1420 1430 1440 1450
PPSFGGSHCE LYTAPTSTPP ATCQSQYCAD KARDGICDEA CNSHACQWDG
1460 1470 1480 1490 1500
GDCSLTMEDP WANCTSTLRC WEYINNQCDE QCNTAECLFD NFECQRNSKT
1510 1520 1530 1540 1550
CKYDKYCADH FKDNHCDQGC NSEECGWDGL DCASDQPENL AEGTLIIVVL
1560 1570 1580 1590 1600
LPPEQLLQDS RSFLRALGTL LHTNLRIKQD SQGALMVYPY FGEKSAAMKK
1610 1620 1630 1640 1650
QKMTRRSLPE EQEQEQEVIG SKIFLEIDNR QCVQDSDQCF KNTDAAAALL
1660 1670 1680 1690 1700
ASHAIQGTLS YPLVSVFSEL ESPRNAQLLY LLAVAVVIIL FFILLGVIMA
1710 1720 1730 1740 1750
KRKQAWLPLA AGRFTLRRDS SNHKRREPVG QDAVGLKNLS VQVSEANLIG
1760 1770 1780 1790 1800
SGTSEHWVDD EGPQPKKAKA EDEALLSEDD PIDRRPWTQQ HLEAADISHT
1810 1820 1830 1840 1850
PSLALTPPQA EQEVDVLDVN VRGPDGCTPL MLASLRGGSS DLSDEDEDAE
1860 1870 1880 1890 1900
DSSANIITDL VYQGASLQAQ TDRTGEMALH LAARYSRADA AKRLLDAGAD
1910 1920 1930 1940 1950
RNAQDNMGRC PLHAAVAGDA QGVFQILIRN RVTDLDARMN DGTTPLILAA
1960 1970 1980 1990 2000
RLAVEGMVAE LINCQADVNA VDDHGKSALH WAAAVNNVEA TLLLLKNGAN
2010 2020 2030 2040 2050
RDMQDNKEET PLFLAAREGS YEAAKILLDH FANRDITDHM DRLPRDVARD
2060 2070 2080 2090 2100
RMHHDIVRLL DEYNVTPSPP GTVLTSALSP VLCGPNRSFL SLKHTPMGKK
2110 2120 2130 2140 2150
ARRPNTKSTM PTSLPNLAKE AKDAKGSRRK KCLNEKVQLS ESSVTLSPVD
2160 2170 2180 2190 2200
SLESPHTYVS DATSSPMITS PGILQASPTP LLAAAAPAAP VHTQHALSFS
2210 2220 2230 2240 2250
NLHDMQPLAP GASTVLPSVS QLLSHHHIAP PGSSSAGSLG RLHPVPVPAD
2260 2270 2280 2290 2300
WMNRVEMNET QYSEMFGMVL APAEGAHPGI AAPQSRPPEG KHMSTQREPL
2310 2320 2330 2340 2350
PPIVTFQLIP KRSIAQAAGA PQTQSSCPPA VAGPLPSMYQ IPEMPRLPSV
2360 2370 2380 2390 2400
AFPPTMMPQQ EGQVAQTIVP TYHPFPASVG KYPTPPSQHS YASSNAAERT
2410 2420 2430 2440 2450
PSHGGHLQGE HPYLTPSPES PDQWSSSSPH SASDWSDVTT SPTPGGGGGG
2460 2470
QRGPGTHMSE PPHSNMQVYA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-3851 Collapse assay details

Data source: Panorama

Official Gene Symbol
Notch2
Peptide Sequence
ALGTLLHTNLR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1566
Peptide End
1576
CPTAC ID
non-CPTAC-3851
Peptide Molecular Mass
1,207.7037
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 4.7 7.3 2.2 6.7 8.3 7.5 8.2 11.1 7.8 15 15 15
y6 (1+) 10.8 7.2 6.7 22.7 10.3 10.1 25.1 12.6 12.1 15 15 15
y5 (1+) 8.6 5.1 4.7 13.4 8.5 6.4 15.9 9.9 7.9 15 15 15
y4 (1+) 15.9 3.7 7.2 11.9 7.3 8.3 19.9 8.2 11 15 15 15
y10 (2+) 17.1 6.1 8.4 16.4 8 9.7 23.7 10.1 12.8 15 15 15
sum 3.9 5.7 2.1 5.9 7.5 7.1 7.1 9.4 7.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments