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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Lgals3bp Cycap, Mama
Sequence Length (AA) Molecular Weight (Da)
577 64491
Protein Name
Galectin-3-binding protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MALLWLLSVF LLVPGTQGTE DGDMRLVNGA SANEGRVEIF YRGRWGTVCD
60 70 80 90 100
NLWNLLDAHV VCRALGYENA TQALGRAAFG PGKGPIMLDE VECTGTESSL
110 120 130 140 150
ASCRSLGWMV SRCGHEKDAG VVCSNDTTGL HILDLSGELS DALGQIFDSQ
160 170 180 190 200
QGCDLFIQVT GQGYEDLSLC AHTLILRTNP EAQALWQVVG SSVIMRVDAE
210 220 230 240 250
CMPVVRDFLR YFYSRRIEVS MSSVKCLHKL ASAYGATELQ DYCGRLFATL
260 270 280 290 300
LPQDPTFHTP LDLYAYARAT GDSMLEDLCV QFLAWNFEPL TQSESWSAVP
310 320 330 340 350
TTLIQALLPK SELAVSSELD LLKAVDQWST ETIASHEDIE RLVEQVRFPM
360 370 380 390 400
MLPQELFELQ FNLSLYQDHQ ALFQRKTMQA LEFHTVPVEV LAKYKGLNLT
410 420 430 440 450
EDTYKPRLYT SSTWSSLVMA STWRAQRYEY NRYNQLYTYG YGSVARYNSY
460 470 480 490 500
QSFQTPQHPS FLFKDKQISW SATYLPTMQS CWNYGFSCTS NELPVLGLTT
510 520 530 540 550
SSYSNPTIGY ENRVLILCGG YSVVDVTSFE GSKAPIPTAL DTNSSKTPSL
560 570 577
FPCASGAFSS FRVVIRPFYL TNSTDMV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-670 Collapse assay details

Data source: Panorama

Official Gene Symbol
LGALS3BP
Peptide Modified Sequence
ALGFEN[+1.0]ATQALGR
Modification Type
Deamidated (NQ)
Protein - Site of Modification
69
Peptide - Site of Modification
6
Peptide Start
64
Peptide End
76
CPTAC ID
CPTAC-670
Peptide Molecular Mass
1,347.6782
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive (ThermoFisher)
Internal Standard
synthetic peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Dionex UltiMate 3000 RSLCnano LC (ThermoFisher)
Column Packing
Acclaim PepMap100 C18, 5um (Trap); Acclaim PepMap RSLC C18, 2um (Analytical)
Column Dimensions
300um x 5mm (Trap); 75um x 25cm (Analytical)
Flow Rate
0.5 uL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 6.8 4.9 6.8 7.4 5.8 6.5 10 7.6 9.4 15 15 15
y9 (1+) 9 5 6 9.4 6.1 4.7 13 7.9 7.6 15 15 15
y8 (1+) 7 6 5.1 6.7 6.3 5.6 9.7 8.7 7.6 15 15 15
sum 7.2 5.2 5.5 7.3 5.8 5.2 10.3 7.8 7.6 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-534 Collapse assay details

Data source: Panorama

Official Gene Symbol
LGALS3BP
Peptide Sequence
ELSEALGQIFDSQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
138
Peptide End
151
CPTAC ID
CPTAC-534
Peptide Molecular Mass
1,591.7842
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 19.7 22.9 13.5 23.6 25.9 19.4 30.7 34.6 23.6 15 25 15
y8 (1+) 14.2 11.4 14.4 22.2 15.1 21.9 26.4 18.9 26.2 15 25 15
y7 (1+) 32.9 25.2 23.4 32.1 26.3 28.6 46 36.4 37 15 25 15
sum 14.5 13.8 12.5 21.6 16.3 19.9 26 21.4 23.5 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-535 Collapse assay details

Data source: Panorama

Official Gene Symbol
LGALS3BP
Peptide Sequence
SDLAVPSELALLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
311
Peptide End
323
CPTAC ID
CPTAC-535
Peptide Molecular Mass
1,354.7708
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 23.7 10 6 24.5 10.1 10.1 34.1 14.2 11.7 15 25 15
y9 (1+) 8.3 8.4 9.9 13.2 8.5 7.7 15.6 12 12.5 15 25 15
y8 (1+) 8 6.5 5 16.6 7.4 9.8 18.4 9.8 11 15 25 15
sum 7.6 6.5 5.3 15.6 7.2 9 17.4 9.7 10.4 15 25 15


Additional Resources and Comments


Assay Details for non-CPTAC-1125 Collapse assay details

Data source: Panorama

Official Gene Symbol
LGALS3BP
Peptide Sequence
ELSEALGQIFDSQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
138
Peptide End
151
CPTAC ID
non-CPTAC-1125
Peptide Molecular Mass
1,591.7842
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 19.1 9 10.3 16.3 14.6 13.1 25.1 17.2 16.7 15 15 15
y8 (2+) 18 3.7 2.1 24.7 30.8 16.6 30.6 31 16.7 10 9 7
y3 (1+) 8.4 8 9.2 10.8 16.1 11 13.7 18 14.3 15 15 15
y4 (1+) 14.4 7.9 3.6 13.6 16.3 11.4 19.8 18.1 12 15 15 15
y5 (1+) 8.8 6.8 8.2 13.1 19.4 12.8 15.8 20.6 15.2 15 15 15
sum 9.3 6.2 8.6 9.7 19.4 12.8 13.4 20.4 15.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2670 Collapse assay details

Data source: Panorama

Official Gene Symbol
LGALS3BP
Peptide Sequence
SDLAVPSELALLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
311
Peptide End
323
CPTAC ID
non-CPTAC-2670
Peptide Molecular Mass
1,354.7708
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 4.6 4.8 6.9 10.9 8 9.4 11.8 9.3 11.7 15 15 15
b3 (1+) 7.5 4.5 8.5 11.1 7.5 11 13.4 8.7 13.9 15 15 15
b5 (1+) 6 4.6 6.5 12.4 7.7 9 13.8 9 11.1 15 15 15
b2 (1+) 5.5 5.3 7 11.9 8.8 8.7 13.1 10.3 11.2 15 15 15
b4 (1+) 4.4 5.3 7 13.3 7.2 8.6 14 8.9 11.1 15 15 15
sum 4.8 4.1 6.7 11.5 7.4 9.2 12.5 8.5 11.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3924 Collapse assay details

Data source: Panorama

Official Gene Symbol
Lgals3bp
Peptide Sequence
SELAVSSELDLLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
311
Peptide End
323
CPTAC ID
non-CPTAC-3924
Peptide Molecular Mass
1,402.7555
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 3.8 2.4 2.3 10.3 6.2 4.9 11 6.6 5.4 15 15 15
y9 (1+) 6.3 3.1 2.8 6.8 4.9 6.5 9.3 5.8 7.1 15 15 15
y8 (1+) 3.5 2.1 2.8 5 6.4 5.9 6.1 6.7 6.5 15 15 15
b2 (1+) 3.3 2.4 2 5.6 6.9 5.6 6.5 7.3 5.9 15 15 15
b3 (1+) 4 2.3 2.6 7 5.6 7.1 8.1 6.1 7.6 15 15 15
sum 2.1 0.9 1.5 5.1 5.7 5.7 5.5 5.8 5.9 15 15 15


Additional Resources and Comments