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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Atic

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Atic Purh
Sequence Length (AA) Molecular Weight (Da)
592 64217
Protein Name
Bifunctional purine biosynthesis protein PURH
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAPSQLALFS VSDKTGLVEF ARSLASLGLS LVASGGTAKA IRDAGLAVRD
60 70 80 90 100
VSELTGFPEM LGGRVKTLHP AVHAGILARN IPEDAADMAR LDFNLVRVVV
110 120 130 140 150
CNLYPFVKTV ASPDVTVEAA VEQIDIGGVT LLRAAAKNHA RVTVVCEPED
160 170 180 190 200
YAGVAAEMHG SDSKDTSLET RRHLALKAFT HTAQYDEAIS DYFRKQYSKG
210 220 230 240 250
ISQMPLRYGM NPHQTPAQLY TLKPKLPITV LNGAPGFINL CDALNAWQLV
260 270 280 290 300
TELRGAVDIP AAASFKHVSP AGAAVGVPLS EDEARVCMVY DLYPTLTPLA
310 320 330 340 350
VAYARARGAD RMSSFGDFVA LSDICDVPTA KIISREVSDG IVAPGYEEEA
360 370 380 390 400
LKILSKKKNG NYCVLQMDQS YKPDENEVRT LFGLRLSQKR NNGVVDKSLF
410 420 430 440 450
SNIVTKNKDL PESALRDLIV ATVAVKYTQS NSVCYAKDGQ VIGIGAGQQS
460 470 480 490 500
RIHCTRLAGD KANSWWLRHH PRVLSMKFKA GVKRAEISNA IDQYVTGTIG
510 520 530 540 550
EGEDLVKWEA LFEEVPELLT EAEKKEWVDK LSGVSVSSDA FFPFRDNVDR
560 570 580 590 592
AKRSGVAYIV APSGSTADKV VIEACDELGI VLAHTDLRLF HH

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3941 Collapse assay details

Data source: Panorama

Official Gene Symbol
Atic
Peptide Sequence
HVSPAGAAVGVPLSEDEAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
267
Peptide End
285
CPTAC ID
non-CPTAC-3941
Peptide Molecular Mass
1,860.9330
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 6.3 3.8 2.9 8.2 7 4 10.3 8 4.9 15 15 15
b11 (2+) 10.9 3.9 2.5 11.6 5.7 4.8 15.9 6.9 5.4 15 15 15
b10 (2+) 9.8 3.3 4.4 9.6 5.9 6.9 13.7 6.8 8.2 15 15 15
b9 (2+) 14.5 4.2 3.9 13.3 6.3 6.6 19.7 7.6 7.7 15 15 15
y8 (2+) 13.3 4.1 4.3 13.8 7.5 5.8 19.2 8.5 7.2 15 15 15
sum 6.7 1.2 1.4 6.8 5.5 3.8 9.5 5.6 4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for non-CPTAC-3870 Collapse assay details

Data source: Panorama

Official Gene Symbol
Atic
Peptide Sequence
TLHPAVHAGILAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
67
Peptide End
79
CPTAC ID
non-CPTAC-3870
Peptide Molecular Mass
1,354.7834
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 19.6 8.2 6.8 54.6 15.3 6.5 58 17.4 9.4 15 15 15
b3 (1+) 8.5 5.5 8.3 10.6 9.7 9.8 13.6 11.2 12.8 15 15 15
y11 (3+) 7.8 8.4 3.8 10.8 12.3 4.2 13.3 14.9 5.7 15 15 15
y10 (2+) 5.8 7.1 6.3 6.8 9.7 8.2 8.9 12 10.3 15 15 15
y5 (1+) 15.2 8 10 25 12.3 11.3 29.3 14.7 15.1 15 15 15
sum 4.8 3.1 2.5 12.1 7.7 3.3 13 8.3 4.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments