Psmb1, CPTAC-3943 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Psmb1	N/A

Sequence Length (AA)	Molecular Weight (Da)
240	26372

Protein Name
Proteasome subunit beta type-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MLSTAAYRDV	ERELGMGPHG	SAGPVQLRFS	PYAFNGGTVL	AIAGEDFSIV
60	70	80	90	100
ASDTRLSEGF	SIHTRDSPKC	YKLTDKTVIG	CSGFHGDCLT	LTKIIEARLK
110	120	130	140	150
MYKHSNNKAM	TTGAIAAMLS	TILYSRRFFP	YYVYNIIGGL	DEEGKGAVYS
160	170	180	190	200
FDPVGSYQRD	SFKAGGSASA	MLQPLLDNQV	GFKNMQNVEH	VPLTLDRAMR
210	220	230	240
LVKDVFISAA	ERDVYTGDAL	RICIVTKEGI	REETVPLRKD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-3943 Collapse assay details

Data source: Panorama

Official Gene Symbol: Psmb1
Peptide Sequence: EETVPLR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 232
Peptide End: 238

CPTAC ID: non-CPTAC-3943
Peptide Molecular Mass: 842.4498
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	12	5.5	4	17.7	9.2	7.4	21.4	10.7	8.4	15	15	15
y4 (1+)	39.2	7.2	6.2	36.6	8.7	4.7	53.6	11.3	7.8	15	15	15
y3 (1+)	7.9	3.4	2.7	9.6	9.1	5	12.4	9.7	5.7	15	15	15
y5 (2+)	34.1	8.2	7.2	29.6	12.9	9.2	45.2	15.3	11.7	15	15	15
b2 (1+)	20.9	8.1	4.5	28	10.5	5.9	34.9	13.3	7.4	15	15	15
sum	7	2.4	2.1	9.8	8.4	4.8	12	8.7	5.2	15	15	15

Psmb1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3943 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Psmb1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-3943 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments