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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Ccdc88b

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ccdc88b Ccdc88
Sequence Length (AA) Molecular Weight (Da)
1481 166608
Protein Name
Coiled-coil domain-containing protein 88B
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEGAKGPRLR GFLSGSLATW ALGLAGLVGE AEESAGGTEE EEEEEEEEGA
60 70 80 90 100
LCTEKRFLRL IDGALLLRVL GIIAPSSRGG LRMVRGRDGP AACRMWNLCH
110 120 130 140 150
LWGRLRDFYQ EELQLLILSP PPDLQTMGCD PFSEEAVDEL ESILRLLLGA
160 170 180 190 200
SVQCEHRELF IRHIRGLSLD VQSELAGAIQ EVTQPGAGVV LALAGPESGE
210 220 230 240 250
LVAEELEMQL RSLTGMMSRL ARERDLGAQR LAELLLEREP AHLLLPEAPA
260 270 280 290 300
NASAEGVSHH LALQLTNAKA QLRRLRQEVE EKAEQLLDSQ AEVQGLEAEI
310 320 330 340 350
RRLRQETQAL SAQAKRAELY REEAEALRER AGRLPRLQEE LRRCREKLQA
360 370 380 390 400
AEVFKGQLEE ERVLSEALEA SKVLLEEQLE VARERSARLH ETQRENLLLR
410 420 430 440 450
TRLGEAHADL DSLRHQLEQL VEENVELELE LQRSLEPPPG SPGEASLPGA
460 470 480 490 500
APSLQDEVRE AEAGRLRAVE RENRELRGQL QMLQAQLGSQ HPLLEEQREN
510 520 530 540 550
SRQPPVPNRD PATPSALHHS PQSPACQIGG EGSESLDLPS PASYSDITRS
560 570 580 590 600
PKCSQAPDSH PELESPLQMV SQDPQTSDQA LQESDPTVET HQCLEKSGHR
610 620 630 640 650
VPLQSPIVWD PPQGPEVRIE VQELLGETGS REAPQGELVH KAQVLKQESP
660 670 680 690 700
KCRPRSAELT LREPLKDQKA LDRELELSKQ QKETGRHEQR PKGLESKLGP
710 720 730 740 750
QKPQQTSEGV PDAWSREEPT PGETLVSAIP EEQALRDEVA QLRREVAGLE
760 770 780 790 800
VKLQAQAQRL EARSAEALCL SEELAQARRT EAEAHQEAEA QAREQARLRE
810 820 830 840 850
AVDTASLELE AASREREALA EALAAAGRER RQWERDGPRL RAQVEAAEQQ
860 870 880 890 900
VQALESQVRC HLEEAEREHA EKQALREELE KAVLRGQELG DRLEHLQEEL
910 920 930 940 950
EQAALERQKF LQEQENQHQR YRHLEQRLEA ELQAASTSKE EALMELKARA
960 970 980 990 1000
LQLEEELIQL RQYPVDLATG ARAGPRTVET QNGRLIEVER NNATLVAEKA
1010 1020 1030 1040 1050
ALQGQLQHLE GQLGSLQGRA QELLLQSQRA QEHSSRLQAE KSMMEMQGQE
1060 1070 1080 1090 1100
LHRKLGVLEE EVRAARRAQE ETRGQQQALL RDHEALVQLQ RRQETELEGL
1110 1120 1130 1140 1150
LVRHRDLKAN MRALELAHRE LQGRHEQLQA QRANVEAQEV ALLAERERLM
1160 1170 1180 1190 1200
QDGHRQRGLE EELRRLQNEH ERAQMLLAEV SRERGELQGE RGELRSRLAR
1210 1220 1230 1240 1250
LELERAQLEI QSQQLRESNQ QLDLSACRLT TQCELLTQLR SAQEEENRQL
1260 1270 1280 1290 1300
LAEVQALSRE NRELLERSLE SRDHLHREQR EYLDQLNALR REKQKLVEKI
1310 1320 1330 1340 1350
MDQYRVLEPG PLPRTKKGSW LADKVKRLIR PRREGALHGG PRLGADGAGS
1360 1370 1380 1390 1400
TESLGGPLET ELPEGREADG TGSSSPAPMR RVQSSLCLGD ETLAGGQRRR
1410 1420 1430 1440 1450
LSSRFPGGRS SASFSPGDTP RQRFRQRRPG PLGAPSTHSK GSGVEWDGSI
1460 1470 1480 1481
KTLSEHEADD TREAFQEQKP EKQFLTPSLS Q

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3967 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ccdc88b
Peptide Sequence
AQVEAAEQQVQALESQVR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
842
Peptide End
859
CPTAC ID
non-CPTAC-3967
Peptide Molecular Mass
1,983.0021
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 3.3 3.3 3 8.9 8.2 6.3 9.5 8.8 7 15 15 15
y4 (1+) 6.9 3 2.4 17.1 7.4 3.4 18.4 8 4.2 15 15 15
b2 (1+) 6 3.9 3.8 8.6 7.7 4.1 10.5 8.6 5.6 15 15 15
b10 (2+) 4 1.9 2.6 10.1 5.1 4.1 10.9 5.4 4.9 15 15 15
b12 (2+) 4.3 3.2 2.4 10.2 7.3 4.1 11.1 8 4.8 15 15 15
sum 4.1 1.5 1 9.5 6.6 3.1 10.3 6.8 3.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments