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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Cd2ap

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cd2ap Mets1
Sequence Length (AA) Molecular Weight (Da)
637 70450
Protein Name
CD2-associated protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVDYIVEYDY DAVHDDELTI RVGEIIRNVK KLQEEGWLEG ELNGRRGMFP
60 70 80 90 100
DNFVKEIKRE TEPKDDNLPI KRERQGNVAS LVQRISTYGL PAGGIQPHPQ
110 120 130 140 150
TKAIKKKTKK RQCKVLFDYS PQNEDELELI VGDVIDVIEE VEEGWWSGTL
160 170 180 190 200
NNKLGLFPSN FVKELESTED GETHNAQEES EVPLTGPTSP LPSPGNGSEP
210 220 230 240 250
APGSVAQPKK IRGIGFGDIF KEGSVKLRTR TSSSETEEKK TEKPLILQPL
260 270 280 290 300
GSRTQNVEVT KPDVDGKIKA KEYCRTLFPY TGTNEDELTF REGEIIHLIS
310 320 330 340 350
KETGEAGWWK GELNGKEGVF PDNFAVQISE LDKDFPKPKK PPPPAKGPAP
360 370 380 390 400
KPDLSAAEKK AFPLKAEEKD EKSLLEQKPS KPAAPQVPPK KPTAPTKASN
410 420 430 440 450
LLRSPGAVYP KRPEKPVPPP PPAAKINGEV SIISSKIDTE PVSKPKLDPE
460 470 480 490 500
QLPVRPKSVD LDAFVARNSK ETDDVNFDDI ASSENLLHLT ANRPKMPGRR
510 520 530 540 550
LPGRFNGGHS PTQSPEKTLK LPKEDDSGNL KPLEFKKDAS YSSKSSLSTP
560 570 580 590 600
SSASKVNTAA FLTPLELKAK AEADDGKRNS VDELRAQIIE LLCIVDALKK
610 620 630 637
DHGKELEKLR KELEEEKAMR SNLEVEIAKL KKAVLLS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3968 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cd2ap
Peptide Sequence
ISTYGLPAGGIQPHPQTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
85
Peptide End
102
CPTAC ID
non-CPTAC-3968
Peptide Molecular Mass
1,863.9843
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 10.7 4.4 5.7 13.6 8.8 6.6 17.3 9.8 8.7 15 15 15
y4 (1+) 8.3 4.3 5.2 8.1 7.1 5.2 11.6 8.3 7.4 15 15 15
y16 (2+) 9 6.3 6.9 14.3 8.1 8.3 16.9 10.3 10.8 15 15 15
y15 (2+) 9.6 7.1 8.6 12.8 10.8 9.6 16 12.9 12.9 15 15 15
y14 (2+) 8.2 3.1 5.4 13.3 5.3 7.3 15.6 6.1 9.1 15 15 15
sum 4.7 1.3 3.1 8.6 4.8 3.4 9.8 5 4.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments