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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

C1sb

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
C1sb C1s, Gm5077
Sequence Length (AA) Molecular Weight (Da)
688 76700
Protein Name
Complement C1s-B subcomponent
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWYLVLFSLL ASFSAEPTMH GEILSPNYPQ AYPNDVVKSW DIEVPEGFGI
60 70 80 90 100
HLYFTHVDIE PSESCAYDSV QIISGGIEEG RLCGQRTSKS PNSPIIEEFQ
110 120 130 140 150
FPYNKLQVVF TSDFSIEEQF TGFAAYYTAI DVNECTDFTD VPCSHFCNNF
160 170 180 190 200
IGGYFCSCPP EYFLHDDMRN CGVNCSGDVF TALIGEISSP NYPNPYPENS
210 220 230 240 250
RCEYQIQLQE GFQVVVTMQR EDFDVEPADS EGNCPDSLTF AAKNQQFGPY
260 270 280 290 300
CGDGFPGPLT IRTQSNTLGI VFQTDLMGQK KGWKLRYHGD PISCPKESTA
310 320 330 340 350
NSNWEPDKAK YVFKDVVKIT CVDGFEVVEG HVSSTSYYST CQSDGQWSNS
360 370 380 390 400
GLKCQPVYCG IPDPIANGKV EEPENSVFGT VIHYTCEEPY YYMEHEEGGE
410 420 430 440 450
YRCAANGRWV NDQLGIELPR CIPVCGVPTE PFQVQQKIFG GQPAKIENFP
460 470 480 490 500
WQVFFNHPTA GGALINEYWV LTAAHVVEKN SDPSMYAGIT ALRLADLENA
510 520 530 540 550
QRLYTKRVII HPGWKEDDDL NPRTNFDNDI ALVQLKDPVK MGPKFSPICL
560 570 580 590 600
PGTSSEYNLS PGDMGLISGW GRTEKRLHVI NLRGAKVPVT SLETCKQVKE
610 620 630 640 650
ENPTARPEDY VITDNMICAG EKGVDSCKGD SGGAFAFQVP NVKAPKFYVA
660 670 680 688
GLVSWGKKCG AYGVYTKVKN YVDWILKTMQ ENSGPRKD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3971 Collapse assay details

Data source: Panorama

Official Gene Symbol
C1sb
Peptide Sequence
GDSGGAFAFQVPNVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
629
Peptide End
643
CPTAC ID
non-CPTAC-3971
Peptide Molecular Mass
1,492.7310
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 9 4.6 8.1 10.1 5.5 11.1 13.5 7.2 13.7 15 15 15
y8 (1+) 15.2 5.4 7.7 16.6 7.2 16.7 22.5 9 18.4 15 15 15
y7 (1+) 11.3 6.4 9.8 16.1 8.4 10.2 19.7 10.6 14.1 15 15 15
y4 (1+) 4.7 2.9 5.4 9 7.6 11.5 10.2 8.1 12.7 15 15 15
b6 (1+) 12 5.9 7.3 18.5 9 14 22.1 10.8 15.8 15 15 15
sum 4.2 2.9 4.7 7.8 6.7 11.3 8.9 7.3 12.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments