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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Cd55

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Cd55 Cd55a, Daf, Daf1
Sequence Length (AA) Molecular Weight (Da)
390 42618
Protein Name
Complement decay-accelerating factor, GPI-anchored
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MIRGRAPRTR PSPPPPLLPL LSLSLLLLSP TVRGDCGPPP DIPNARPILG
60 70 80 90 100
RHSKFAEQSK VAYSCNNGFK QVPDKSNIVV CLENGQWSSH ETFCEKSCVA
110 120 130 140 150
PERLSFASLK KEYLNMNFFP VGTIVEYECR PGFRKQPPLP GKATCLEDLV
160 170 180 190 200
WSPVAQFCKK KSCPNPKDLD NGHINIPTGI LFGSEINFSC NPGYRLVGVS
210 220 230 240 250
STFCSVTGNT VDWDDEFPVC TEIHCPEPPK INNGIMRGES DSYTYSQVVT
260 270 280 290 300
YSCDKGFILV GNASIYCTVS KSDVGQWSSP PPRCIEKSKV PTKKPTINVP
310 320 330 340 350
STGTPSTPQK PTTESVPNPG DQPTPQKPST VKVSATQHVP VTKTTVRHPI
360 370 380 390
RTSTDKGEPN TGGDRYIYGH TCLITLTVLH VMLSLIGYLT

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3972 Collapse assay details

Data source: Panorama

Official Gene Symbol
Cd55
Peptide Sequence
VSATQHVPVTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
333
Peptide End
343
CPTAC ID
non-CPTAC-3972
Peptide Molecular Mass
1,165.6455
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (2+) 23.6 9.1 5.9 31.4 12.9 8.1 39.3 15.8 10 15 15 15
y9 (2+) 7 5.1 3.5 8 5.6 6.4 10.6 7.6 7.3 15 15 15
y10 (2+) 8.8 3.6 6.3 17 10.3 9.7 19.1 10.9 11.6 15 15 15
y2 (1+) 8.9 6.5 5.4 11.3 9.3 6.5 14.4 11.3 8.5 15 15 15
y4 (1+) 11.2 7.5 3.9 15.6 10.1 5.4 19.2 12.6 6.7 15 15 15
sum 5.5 2.3 3.8 12.6 7.1 7.4 13.7 7.5 8.3 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments