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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Ecpas

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ecpas Ecm29, Kiaa0368
Sequence Length (AA) Molecular Weight (Da)
1840 203703
Protein Name
Proteasome adapter and scaffold protein ECM29
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
METGSDSDQL ERVFLRLGHA ETDEQLQNII SKFLPPVLLK LSSTQEGVRK
60 70 80 90 100
KVMELLVHLN KRIKSRPKIQ LPVETLLVQY QDPAAVSFVT NFTIIYVKMG
110 120 130 140 150
YPRLPVEKQC ELAPTLLTAM EGKPQPQQDS LMHLLIPTLF HMKYPAESSK
160 170 180 190 200
SASPFNLAEK PKTVQLLLDF MLDVLLMPYG YVLNESQSRQ NSSSSSQGSS
210 220 230 240 250
SNSGGGSGIP QPPPGMSFYA AKRVIGDNPW TPEQLEQCKL GIVKFIEAEQ
260 270 280 290 300
VPELEAVLHL VIASSDTRHS VATAADLELK SKQSLIDWNN PAIINKMYKV
310 320 330 340 350
YLGDIPLKTK EGAVLKPELK RDPVSTRVKL KIVPHLLRSR QAAETFPANI
360 370 380 390 400
QVVYDGLFGT NTNSKLRTLS LQFVHHICLT CPEIKIKPLG PMLLNGLTKL
410 420 430 440 450
INEYKEDPKL LSMAYSAVGK LSSRMPHLFT KDIALVQQLF EALCKEEPET
460 470 480 490 500
RLAIQEALSM MVGAYSTLEG AQRTLMEALV ASYLIKPEVQ VRQVAVKFAS
510 520 530 540 550
TVFPSDHIPS RYLLLLAAGD PREEVHGEAQ RVLRCLPGRN KKESASKQMP
560 570 580 590 600
SFPEMVYYIQ EKASHRMKTP VKYMTGTTVL PFNPAAFGEI VLYLRMCLAH
610 620 630 640 650
SAGVVPTSQS LADMQDHAPA IGRYIRALMS SSQATASSSN KSGETNPVQI
660 670 680 690 700
YTGLLQQLLA GVGGLPVMYC LLEAVSVYPE KLATKFVDKT EWIKSLMSSS
710 720 730 740 750
KEEMRELAAL FYSVVVSTVS GIELKSMIEQ LIKATKDNHS PEVQHGSLLA
760 770 780 790 800
LGFTVGRYLA KKRVRMAEQH DLETDADLLP EQEEIIRSAT ETIGSFLDST
810 820 830 840 850
SPLLAIAACT ALGEIGRNGP LPIPSEGSGF TKLHLVESLL NRIPSSKETN
860 870 880 890 900
KMKERAIQTL GYFPVGDGVF PHQKLLLQGL MDSVEAKQIE LQFTIGEAIT
910 920 930 940 950
SAAIGTNSVA ARDAWLVTEE EYIPPAGAKV NDVVPWVLDV ILNKHIISPN
960 970 980 990 1000
PHVRQAACIW LLSLVRKLST HREVKSHLKE IQSAFVSVLS ENDELSQDVA
1010 1020 1030 1040 1050
SKGLGLVYEL GNEQDQQELV STLVETLMTG KRVKHEVSGE TVVFQGGGLG
1060 1070 1080 1090 1100
KTPDGQGLST YKELCSLASD LSQPDLVYKF MNLANHHAMW NSRKGAAFGF
1110 1120 1130 1140 1150
NVIATRAGEQ LAPFLPQLVP RLYRYQFDPN LGIRQAMTSI WNALVTDKSM
1160 1170 1180 1190 1200
VDKYLKEILQ DLIKNLTSNM WRVRESSCLA LNDLLRGRPL DDVIDKLPEM
1210 1220 1230 1240 1250
WETLFRVQDD IKESVRKAAE LALKTLSKVC VKMCDPAKGA AGQRTIAVLL
1260 1270 1280 1290 1300
PCLLDKGMMS PVTEVRALSI NTLVKISKSA GAMLKPHAPK LIPALLESLS
1310 1320 1330 1340 1350
VLEPQVLNYL SLRATEQEKD VMDSARLSAA KSSPMMETIN MCLQYLDVSV
1360 1370 1380 1390 1400
LGELVPRLCE LIRSGVGLGT KGGCASVIVS LTTQCPQDLT PYSGKLMSAL
1410 1420 1430 1440 1450
LSGLTDRNSV IQKSCAFAMG HLVRTSRDSS TEKLLQKLNG WYMEKDEPVY
1460 1470 1480 1490 1500
KTSCALTIHA IGRYSPDVLK NHAKEVLPLA FLGMHEIADE EKSEKEECNM
1510 1520 1530 1540 1550
WTEVWQENVP GSFGGIRLYL QELITITQKA LQSQSWKMKA QGAIAMASIS
1560 1570 1580 1590 1600
KQTSSLVPPY LGMILSALMQ GLAGRTWAGK EELLKAIACV VTACSTELEK
1610 1620 1630 1640 1650
SVPNQPTTNE ILQAVLKECC KENLKYKIVA ISCAADVLKA TKEDRFQEFS
1660 1670 1680 1690 1700
DIVIPLIKKN SLESMGVRTT KAEDENEKER ELQLESLLGA FESLGKAWPR
1710 1720 1730 1740 1750
NPDTQRCYRQ ELCKLMCERL RLSTWKVQLG VLQSMNAFFQ GLMLLEEEHA
1760 1770 1780 1790 1800
DPEALAEILL ETCKSITYSL ENKTYSSVRT EALSVVELLL KKLEEAKQWE
1810 1820 1830 1840
SLTAECRGLL IESLATMETD NRPELQEKAS VLKKTLESLE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3973 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ecpas
Peptide Sequence
TEALSVVELLLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1780
Peptide End
1791
CPTAC ID
non-CPTAC-3973
Peptide Molecular Mass
1,313.7806
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 6.4 3.8 1.3 9.4 8.5 6.2 11.4 9.3 6.3 15 15 15
b4 (1+) 5.4 3.1 2.3 10.5 7.6 5.6 11.8 8.2 6.1 15 15 15
b2 (1+) 3.6 2.4 1.2 9.5 7.3 6 10.2 7.7 6.1 15 15 15
y6 (1+) 4.9 3.6 1.5 12.4 8.1 4.5 13.3 8.9 4.7 15 15 15
y8 (1+) 8 3.9 1.1 9.8 9.7 5.5 12.7 10.5 5.6 15 15 15
sum 3.6 1.5 0.6 9.1 7.5 5.5 9.8 7.6 5.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments