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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Dspp

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Dspp Dmp3
Sequence Length (AA) Molecular Weight (Da)
934 93903
Protein Name
Dentin sialophosphoprotein
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKMKIIIYIC IWATAWAIPV PQLVPLERDI VENSVAVPLL THPGTAAQNE
60 70 80 90 100
LSINSTTSNS NDSPDGSEIG EQVLSEDGYK RDGNGSESIH VGGKDFPTQP
110 120 130 140 150
ILVNEQGNTA EEHNDIETYG HDGVHARGEN STANGIRSQV GIVENAEEAE
160 170 180 190 200
SSVHGQAGQN TKSGGASDVS QNGDATLVQE NEPPEASIKN STNHEAGIHG
210 220 230 240 250
SGVATHETTP QREGLGSENQ GTEVTPSIGE DAGLDDTDGS PSGNGVEEDE
260 270 280 290 300
DTGSGDGEGA EAGDGRESHD GTKGQGGQSH GGNTDHRGQS SVSTEDDDSK
310 320 330 340 350
EQEGFPNGHN GDNSSEENGV EEGDSTQATQ DKEKLSPKDT RDAEGGIISQ
360 370 380 390 400
SEACPSGKSQ DQGIETEGPN KGNKSIITKE SGKLSGSKDS NGHQGVELDK
410 420 430 440 450
RNSPKQGESD KPQGTAEKSA AHSNLGHSRI GSSSNSDGHD SYEFDDESMQ
460 470 480 490 500
GDDPKSSDES NGSDESDTNS ESANESGSRG DASYTSDESS DDDNDSDSHA
510 520 530 540 550
GEDDSSDDSS GDGDSDSNGD GDSESEDKDE SDSSDHDNSS DSESKSDSSD
560 570 580 590 600
SSDDSSDSSD SSDSSDSSDS SDSSDSSDSS DSSDSNSSSD SSDSSGSSDS
610 620 630 640 650
SDSSDTCDSS DSSDSSDSSD SSDSSDSSDS SDSSDSSDSS DSSSSSDSSD
660 670 680 690 700
SSSCSDSSDS SDSSDSSDSS DSSDSSSSDS SSSSNSSDSS DSSDSSSSSD
710 720 730 740 750
SSDSSDSSDS SDSSGSSDSS DSSASSDSSS SSDSSDSSSS SDSSDSSDSS
760 770 780 790 800
DSSDSSESSD SSNSSDSSDS SDSSDSSDSS DSSDSSDSSD SSNSSDSSDS
810 820 830 840 850
SDSSDSSDSS NSSDSSDSSD SSDSSDSSDS SDSSDSSDSS DSSDSSDSSD
860 870 880 890 900
SSDSSDSSDS SDSSDSSDSS DSSDSSDSSN SSDSSDSDSK DSSSDSSDGD
910 920 930 934
SKSGNGNSDS NSDSNSDSDS DSEGSDSNHS TSDD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-3974 Collapse assay details

Data source: Panorama

Official Gene Symbol
Dspp
Peptide Sequence
SQDQGIETEGPNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
359
Peptide End
371
CPTAC ID
non-CPTAC-3974
Peptide Molecular Mass
1,401.6372
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 6.7 5.1 5.4 8.5 4.9 4.1 10.8 7.1 6.8 15 15 15
b5 (1+) 30.9 19.7 16.5 37.3 19.5 20.1 48.4 27.7 26 15 15 15
b4 (1+) 26.7 15.3 12.1 28.6 13.6 12.5 39.1 20.5 17.4 15 15 15
b7 (1+) 21.1 22.4 14.6 25.5 18.4 17.7 33.1 29 22.9 15 15 15
b3 (1+) 19.1 5.6 7 41.8 8.6 8.2 46 10.3 10.8 15 15 15
sum 8.6 4.3 3.4 10.6 3.7 3.4 13.6 5.7 4.8 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments