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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ptpdc1 N/A
Sequence Length (AA) Molecular Weight (Da)
747 83934
Protein Name
Protein tyrosine phosphatase domain-containing protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAAGVLPQNE DPYSTLVNSS GHAAHMDENS GRPAPKYTKV GERLRHVIPG
60 70 80 90 100
HMACSMACGG RACKYENPAR WSEQEQAIKG VYSSWVTDNI LAMARPSSEL
110 120 130 140 150
LEKYRIIEQF LGQGIKTIIN LQRPGEHASC GSALEQESGF TYLPEAFMEA
160 170 180 190 200
GIYFYNFGWK DYGVASLTAI LDMVKVMTFA LQEGKVAVHC HAGLGRTGVL
210 220 230 240 250
IACYLVFATR MTADQAIIFV RAKRPNSIQT RGQLLCVREF TQFLAPLRNI
260 270 280 290 300
FSCCDPKAHA VTLAQYLIRQ RHLLHGYEAR LLKYVPKIIH LVCKLLLDLA
310 320 330 340 350
ENRPVVMKSM LEGPVLSAEI EKTVSEMVTL QLDQELLRQN SDVPDPFNPT
360 370 380 390 400
AEVAEFENQD VILSTEQEFD PLWKRRDIEC LQPLTHLKRQ LSYSDSDLKR
410 420 430 440 450
AKAILEQGET PWTVPAQELL DHSLQHQKPT SHCYMPPTPE LGFNKEALVQ
460 470 480 490 500
NTFSFWTPSK CGGLEGLKDE GSLLLCRKDI PKEVQRSRTF SVGVSCSHNP
510 520 530 540 550
GEPVPPNFTS IHKDPEQVTH CRCEAPGGWV PGPVHEMVRS PCSPLNCGSS
560 570 580 590 600
PKAQFPHGQE TQDSTDLSEA VPHAGLQPEL SAEARRILAA KALANLNEFV
610 620 630 640 650
EKEEVKRKVE MWQKELNSRE EAWERICGER DPFILCSLMW SWVEQLKEPV
660 670 680 690 700
ITKEDVDMLV DRQADAAEAL FLLEKGQYQT ILCVLHCIVS LQTLPMEVEE
710 720 730 740 747
ACLLHAIKAF TKVNFDSENG PIVYDTLKKI FKHTLEEKRK MAKDSLS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4000 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ptpdc1
Peptide Sequence
ALANLNEFVEK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
592
Peptide End
602
CPTAC ID
non-CPTAC-4000
Peptide Molecular Mass
1,246.6558
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 5.6 3 2.6 6.7 4.3 4.9 8.7 5.2 5.5 15 15 15
y8 (1+) 3.6 2.2 2 6.1 5 4.4 7.1 5.5 4.8 15 15 15
y6 (1+) 3.8 3 2.7 4.3 3.8 4.1 5.7 4.8 4.9 15 15 15
y9 (2+) 7.8 2 2 11.4 4.2 2.9 13.8 4.7 3.5 15 15 15
b3 (1+) 6.4 2.5 3.8 6.3 2.6 3.7 9 3.6 5.3 15 15 15
sum 3.3 1.5 1 4.4 3.6 3.4 5.5 3.9 3.5 15 15 15


Additional Resources and Comments