Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Arhgap18

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Arhgap18 N/A
Sequence Length (AA) Molecular Weight (Da)
663 74930
Protein Name
Rho GTPase-activating protein 18
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNWLSSSSGV VLTAYHPSGK DQVAGDSHVK GGDEATSSRR YGQYTINQEG
60 70 80 90 100
STKVPERPPF DRSSSQDSLD ESMEAYWTEL ENIKRSNENR QEGQEAIVVK
110 120 130 140 150
EPDEGELEEE WLKEAGLSNL FGESIDDPQE SILFLSTLTR TQAAAVQKRV
160 170 180 190 200
ETVSQTLRKK NKQHHIRDVR DIFAQQREAQ EKPPDDSDLR SVRTNENKGQ
210 220 230 240 250
GKDDQPSSGA VDSKEQISRV PEDTPASETD INLEVSFAEQ AVNQKEFSKE
260 270 280 290 300
RTQKISSNDS LPSFRLPKDK TGTTRIGDLA PQDMKKVCSL SLIELTALYD
310 320 330 340 350
VLGLEFKQQK AVKIKTRDSG LFGIPLTILL EQDQRKVPGT RIPLIFQKLI
360 370 380 390 400
SRIEEGSLET EGLLRIPGAA MRIKNLCQEL EAKFYEGTFN WESVKQHDAA
410 420 430 440 450
SLLKLFLREL PQPLLSMEYL KAFQAVQNLP TRKEQLQALN LLVILLPDAN
460 470 480 490 500
RDTLKALLEF LQRVIDNKEK NKMTAGNVAM VMAPNLFMCH TLGLKSSEQR
510 520 530 540 550
EFEMAAGTAN VMHLLIRYQK ILWTIPKFIV IQVRKQNIEN QKKERKAMKK
560 570 580 590 600
LLKKMAYDRE KHEKQDKTAN GADVPQGVIR VQAPHLSKVS MAIQLTEELK
610 620 630 640 650
ASDVLARFLS QESGVAQTLK KGEVFLYEIG GNIGERCLDD DTHMKDLYQL
660 663
NPNAEWVIKS KPV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-4001 Collapse assay details

Data source: Panorama

Official Gene Symbol
Arhgap18
Peptide Sequence
FLSQESGVAQTLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
608
Peptide End
620
CPTAC ID
non-CPTAC-4001
Peptide Molecular Mass
1,406.7405
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 8.3 5.2 6.8 10.9 8 7.9 13.7 9.5 10.4 15 15 15
y9 (1+) 13.8 4 13.3 16.2 5.5 13.3 21.3 6.8 18.8 15 15 15
y8 (1+) 8.9 8.3 3.4 8.6 9.6 6.9 12.4 12.7 7.7 15 15 15
y11 (2+) 11.7 8.7 8.3 12.5 11.5 7.6 17.1 14.4 11.3 15 15 15
b2 (1+) 6.9 6.5 6.9 7.1 6.7 8.1 9.9 9.3 10.6 15 15 15
sum 5.1 2.2 3.8 5.8 4.6 5.4 7.7 5.1 6.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments