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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Vnn3

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Vnn3 N/A
Sequence Length (AA) Molecular Weight (Da)
500 56305
Protein Name
Vascular non-inflammatory molecule 3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MASLHFPQWA VSFVFFAQAV GSMDTFIAAV YEHAVILPNK TESPVSTEEA
60 70 80 90 100
LLLINKNIDI LESAIKLAAR QGAHIIVTPE DGIYGWIFTR ETIYPYLEDI
110 120 130 140 150
PDPEVNWIPC RDPRRFGYTP VQERLSCLAK ENSIYIMANI GDKKPCNATD
160 170 180 190 200
PHCPPDGRYQ YNTNVVFDSK GRLTARYHKY NLFEPEIQFD FPKDSELVTF
210 220 230 240 250
DTPFGKFGIF TCFDIFSYDP AVVVVKDTQV DSVLLPTAWY NTLPLLSAVP
260 270 280 290 300
FHSVWARAMG VNVLAANTHN TSMHMTGSGI YSPEAVRVYH YDMETESGQL
310 320 330 340 350
LLSELRSRPR QHATPAEVNW SAYARTVKPF SSGQADFPGK IYFDEFSFTK
360 370 380 390 400
LTGSAGNYTV CQKDLCCHLT YKMSESRMDE VYVLGAFDGL HTVEGQYYLQ
410 420 430 440 450
ICTLLKCQTT NSRTCGEPVG SAFTKFEEFS LSGTFRTKYV FPQIVLSGSQ
460 470 480 490 500
LALERYYEVS RDGRLRSRGG APLPILVMAL YGRVFERDPP RLGQGPGKLQ

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4002 Collapse assay details

Data source: Panorama

Official Gene Symbol
Vnn3
Peptide Sequence
FEEFSLSGTFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
426
Peptide End
436
CPTAC ID
non-CPTAC-4002
Peptide Molecular Mass
1,318.6194
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 13 2.6 2.1 12.6 5.1 4.6 18.1 5.7 5.1 15 15 15
y9 (1+) 10 2 2.9 11.9 5.1 3 15.5 5.5 4.2 15 15 15
b4 (2+) 7.9 2.9 2.6 8.3 6.1 4.1 11.5 6.8 4.9 15 15 15
b3 (1+) 7.3 6 4.1 10.3 5.6 4.6 12.6 8.2 6.2 15 15 15
y5 (1+) 7 2.2 3.8 9.7 4.8 5.2 12 5.3 6.4 15 15 15
sum 7.4 0.7 1.5 8.8 4.7 3.2 11.5 4.8 3.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments