Serpina11, CPTAC-4011 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Serpina11	Gm895

Sequence Length (AA)	Molecular Weight (Da)
424	47188

Protein Name
Serpin A11

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGPVWLWLWL	LVAEVLLPVH	CQPFSAHGDK	SLGASQPASH	QSLEPAPAYH
60	70	80	90	100
KVTPTITNFA	LRLYKQLAEE	VAGNILFSPV	SLSSSLALLS	LGAHADTQTQ
110	120	130	140	150
ILESLGFNLT	ETPAADVHRG	FQSLLHTLDL	PSPKLELKLG	HSLFLDRQLK
160	170	180	190	200
PQQRFLDSAK	ELYGALAFSA	NFTEAAATGQ	QINDLVRKQT	YGQVVGCLPE
210	220	230	240	250
FSHDTLMVLL	NYIFFKAKWK	HPFDRYQTRK	QESFSLDQRT	PLRIPMMRQK
260	270	280	290	300
EMHRFLYDQE	ASCTVLQIEY	SGTALLLLVL	PDPGKMQQVE	AALQPETLRR
310	320	330	340	350
WGQRFLPSLL	DLHLPRFSIS	ATYNLEEILP	LIGLGNLFDM	EADLSGIMGQ
360	370	380	390	400
LNKTVSRVSH	KAIVDMNEKG	TEAAAASGLL	SQPPALNMTS	APQAHYNRPF
410	420	424
LLLLWEVTTQ	SLLFLGKVVN	PAAG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-4011 Collapse assay details

Data source: Panorama

Official Gene Symbol: Serpina11
Peptide Sequence: LGHSLFLDR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 139
Peptide End: 147

CPTAC ID: non-CPTAC-4011
Peptide Molecular Mass: 1,056.5716
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b4 (1+)	11.5	4.8	6	10.5	8.4	8.1	15.6	9.7	10.1	15	15	15
y3 (2+)	23.6	7	7.8	22.9	11.2	8.3	32.9	13.2	11.4	15	15	15
y8 (2+)	8.8	3.1	3.2	12.3	7.1	4.5	15.1	7.7	5.5	15	15	15
y3 (1+)	4.5	3	2.3	7.9	5.4	3.5	9.1	6.2	4.2	15	15	15
y4 (1+)	6.1	2.9	2.4	11.1	7.9	4.5	12.7	8.4	5.1	15	15	15
sum	3.3	2.2	1.7	8.3	6.1	3.7	8.9	6.5	4.1	15	15	15

Serpina11

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-4011 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Serpina11

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-4011 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments