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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Serpinb5 Pi5, Spi5, Spi7
Sequence Length (AA) Molecular Weight (Da)
375 42111
Protein Name
Serpin B5
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDALRLANSA FAVDLFKQLC ERDPAGNILF SPICLSTSLS LAQVGTKGDT
60 70 80 90 100
ANEIGQVLHF ENVKDVPFGF QTVTSDVNKL SSFYSLKLVK RLYIDKSLNP
110 120 130 140 150
STEFISSTKR PYAKELETVD FKDKLEETKG QINSSIKELT DGHFEDILSE
160 170 180 190 200
NSISDQTKIL VVNAAYFVGK WMKKFPESET KECPFRISKT DTKPVQMMNL
210 220 230 240 250
EATFCLGNID DISCKIIELP FQNKHLSMLI VLPKDVEDES TGLEKIEQQL
260 270 280 290 300
NPETLLQWTN PSTMANAKVK LSLPKFKVEK MIDPKASLES LGLKSLFNES
310 320 330 340 350
TSDFSGMSET KGVSLSNVIH RVCLEITEDG GESIEVPGSR ILQHKDEFNA
360 370 375
DHPFIYIIRH NKTRNIIFFG KFCSP

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-582 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINB5
Peptide Sequence
DVPFGFQTVTSDVNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
65
Peptide End
79
CPTAC ID
CPTAC-582
Peptide Molecular Mass
1,652.8046
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11 (1+) 7.8 8.6 7.6 8.9 11.6 9.3 11.8 14.4 12 15 25 15
y8 (1+) 11.5 10.1 8.6 15.6 13.9 12.7 19.4 17.2 15.3 15 25 15
y13 (2+) 10.8 7.8 5.1 20.7 10.6 7.6 23.3 13.2 9.2 15 25 15
sum 9.4 7.4 4.5 18.8 10.5 6.6 21 12.8 8 15 25 15


Additional Resources and Comments


Assay Details for CPTAC-583 Collapse assay details

Data source: Panorama

Official Gene Symbol
SERPINB5
Peptide Sequence
ILVVNAAYFVGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
159
Peptide End
170
CPTAC ID
CPTAC-583
Peptide Molecular Mass
1,292.7493
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
cell line lysate pool
Submitting Laboratory
Seoul National University / Korea Institute of Science and Technology and FHCRC
Submitting Lab PI
Youngsoo Kim

Publication

View Details (opens in a new window)

Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Response curves acquired at Seoul National University / Korea Institute of Science and Technology. Repeatability data acquired at Fred Hutchinson Cancer Research Center.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
5500 QTRAP (ABSCIEX)
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C,15N
LC
nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent)
Column Packing
ChromXP C18-CL, 3 um, 120A
Column Dimensions
150 x 0.075 mm
Flow Rate
500 nL / min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 15.5 12.1 8.1 21.6 15 18.3 26.6 19.3 20 15 25 15
y9 (1+) 10.8 8.4 9 14.9 13.1 17 18.4 15.6 19.2 15 25 15
y8 (1+) 11.4 10.4 7.2 13.6 14.2 16.9 17.7 17.6 18.4 15 25 15
sum 6.7 7.7 7.4 12.7 12.7 16.5 14.4 14.9 18.1 15 25 15


Additional Resources and Comments


Assay Details for non-CPTAC-4010 Collapse assay details

Data source: Panorama

Official Gene Symbol
Serpinb5
Peptide Sequence
DVPFGFQTVTSDVNK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
65
Peptide End
79
CPTAC ID
non-CPTAC-4010
Peptide Molecular Mass
1,652.8046
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 21.6 6.9 10.1 25.2 10.4 10.4 33.2 12.5 14.5 15 15 15
y8 (1+) 8.8 14.5 12.8 14 13.3 12.8 16.5 19.7 18.1 15 15 15
y2 (1+) 16.7 11.9 8.2 18.6 12.1 10.4 25 17 13.2 15 15 15
y13 (2+) 2.8 2.7 2 7.6 4.5 4.8 8.1 5.2 5.2 15 15 15
b2 (1+) 20.4 9 6.3 20.3 11.9 10.8 28.8 14.9 12.5 15 15 15
sum 3.1 2.1 2 7.6 4.4 4.8 8.2 4.9 5.2 15 15 15


Additional Resources and Comments