Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Dsc3

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Dsc3 N/A
Sequence Length (AA) Molecular Weight (Da)
896 100203
Protein Name
Desmocollin-3
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVVPEFRSPQ CRALCTKLLL TLWVFSFVGE ACKKVTFHVP STLEADKIIG
60 70 80 90 100
RVSLKECLSS ADGIMPSDPD FRVLDDGSVY PTRAVVLSDE KRSFTIQLSD
110 120 130 140 150
SKMQTQKEIP VILEHKKKVL KKRHTKETVL RRSKRRWAPI PCSMQENSLG
160 170 180 190 200
PFPLFLQQVQ SDAAQNYTVF YSISGRGADQ EPLNWFFIER DTGNLYCTRP
210 220 230 240 250
VDREEYDVFD LIAYASTADG YSADLPLPLP IKIEDENDNY PLFTEAIYAF
260 270 280 290 300
EVPEGSRLGT VVGTVCATDK DEPDTMHTRL KYSILEQTPP SPGLFSVHPD
310 320 330 340 350
TGVITTVSHY MDREVVDKYK LIMKVQDMNG QFFGLISTST CIITVQDSND
360 370 380 390 400
NAPTFRQNTY ETAVEENTYN VEILRIPVDD KDMINTANWK ANFTILKGNE
410 420 430 440 450
NGWFKITTDP VTNEGVLCVV KPLDYEENRQ VTLEIGVNNE APFIKDVANR
460 470 480 490 500
IPTMNRAMVT VHVKDQNEGP ECKPPEQYVR IKENSAVGSK INGYKAYDPE
510 520 530 540 550
TKNSNGLRYK KLQDPKDWVS IEEVSGLLTI SKTLDREIMA PRNDMYNITV
560 570 580 590 600
MAIDQEGKSC TGTLAVNIED VNDNAPEIIQ DYIVICKPKM GYTDISAVDP
610 620 630 640 650
DEPIHGPPFQ FNLANTSPEV NRIWTLNQVN DTAARLSYQK TADVQIYNVP
660 670 680 690 700
VTVKDRAGQS ATKILRVNLC DCTHPSQCPL RSRSAGITLG KWAILAILLG
710 720 730 740 750
IALLFSVLLT LVCGVVTARK GKHFPEDLAQ QNLIISNTEA PGDDRVCSAN
760 770 780 790 800
GFTTHTANNS SQGFCGTMGS GMRNGGQETI EMMKGHQTLD SCRVAGHHHT
810 820 830 840 850
LDSGRGGHMD TDNCRYTYSE WHSFTQPRLG EKLHVCNQNE DHIPSQDYVL
860 870 880 890 896
TYNYEGRGSP AGSVGCCSEK QEEEGLDFLN NLEPKFLTLA ETCTKR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-4029 Collapse assay details

Data source: Panorama

Official Gene Symbol
Dsc3
Peptide Sequence
SAGITLGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
684
Peptide End
691
CPTAC ID
non-CPTAC-4029
Peptide Molecular Mass
745.4334
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (2+) 26.5 5 5.3 23.5 5.2 7 35.4 7.2 8.8 15 15 15
y2 (1+) 11.3 6.2 3.2 19.4 6.7 3.6 22.5 9.1 4.8 15 15 15
y3 (1+) 29 7.3 6.2 24.4 7.8 7.3 37.9 10.7 9.6 15 15 15
y4 (1+) 21.4 4.3 4.8 21.2 5.5 7.2 30.1 7 8.7 15 15 15
y6 (1+) 6 4.3 4.2 11.3 7.6 6.9 12.8 8.7 8.1 15 15 15
sum 6.1 3.3 3.3 9.5 6.1 5.3 11.3 6.9 6.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments