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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Golim4

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Golim4 Df10, Golph4
Sequence Length (AA) Molecular Weight (Da)
655 76785
Protein Name
Golgi integral membrane protein 4
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MGNGMCSRKQ KRIFQTLLLL TVVFGFLYGA MLYLELQTQL RKAEAVALKY
60 70 80 90 100
QQHQDSLSAQ LQVVYEHRSR LEKSLQKERL EHKKAKEDFL VYKLEAQETL
110 120 130 140 150
NKGRQDSNSR YSALNVQHQM LKSQHEELRK QHSDLEEEHR KQGEDFSRTF
160 170 180 190 200
NDHKQRYLQL QQEKEQELSK LKETVYNLRE ENRQLRKAHQ DIHTQLQDVK
210 220 230 240 250
TQVAEYKQLK DTLNRIPSFR NPDPVEQQNV TFPHGTHPPQ GYNGREKLTG
260 270 280 290 300
ELQEVQPNHE AGPRRMEEKP LSSMQKDAGF QALEEQNQVE PREPEERQVE
310 320 330 340 350
EEHRKALEEE EMEQVGQAEH LEEEHDPSPE EQDRDWRDQQ GQNAAHLLDG
360 370 380 390 400
HPQAEVEHST KAATNFQSPY EEQLEQQRLA ARRDEEAQRL REHQEALHQQ
410 420 430 440 450
RLHGQLLRQQ QQQQFLAREM AQQKQVAHED GQQQHQEQLR QQAHYNAVEN
460 470 480 490 500
DIAQGVEDQG IPEEEGGAYD RDNQRQDEAE GDPGNRQELR EPGHQEGDPE
510 520 530 540 550
VEADRAAVED INPADDPNNQ GEDEFEEAEQ VREENLPEES EEQKQSEAKQ
560 570 580 590 600
GNVEMDEHLV MAGNPDQQED NVDEQYQDEG EEEVQEDLTE EKKREMEHNV
610 620 630 640 650
EETYGEHPDD KNNDGEEQGV HNRAHPKGRQ EHYEEEEDEE DGAAVAEKSH
655
RRAEM

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4030 Collapse assay details

Data source: Panorama

Official Gene Symbol
Golim4
Peptide Sequence
DAGFQALEEQNQVEPR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
277
Peptide End
292
CPTAC ID
non-CPTAC-4030
Peptide Molecular Mass
1,829.8544
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 13.2 11.2 10.1 16.2 15 12.8 20.9 18.7 16.3 15 15 15
y2 (1+) 6.9 5.3 4.8 7.5 5.9 6.9 10.2 7.9 8.4 15 15 15
y3 (1+) 10.1 6.3 4.6 12.3 8.1 8.4 15.9 10.3 9.6 15 15 15
y6 (1+) 16.2 9 11.1 16.6 13.5 11.3 23.2 16.2 15.8 15 15 15
b5 (1+) 16.8 16.2 12.2 20.3 17 12.1 26.4 23.5 17.2 15 15 15
sum 5.4 4.1 3.7 8.1 6.4 4.7 9.7 7.6 6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments