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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Krt6a Ker2, Krt2-6, Krt2-6a, Krt6
Sequence Length (AA) Molecular Weight (Da)
553 59335
Protein Name
Keratin, type II cytoskeletal 6A
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSTKTTIKSQ TSHRGYSASS ARVPGLNRSG FSSVSVCRSR GSGGSSAMCG
60 70 80 90 100
GAGFGSRSLY GVGSSKRISI GGGSCGIGGG YGSRFGGSFG IGGGAGSGFG
110 120 130 140 150
FGGGAGFGGG YGGAGFPVCP PGGIQEVTIN QSLLTPLNLQ IDPTIQRVRT
160 170 180 190 200
EEREQIKTLN NKFASFIDKV RFLEQQNKVL DTKWALLQEQ GTKTVRQNLE
210 220 230 240 250
PMFEQYISNL RRQLDSIIGE RGRLDSELRN MQDTVEDYKS KYEDEINKRT
260 270 280 290 300
AAENEFVTLK KDVDAAYMNK VELQAKADSL TDDINFLRAL YEAELSQMQT
310 320 330 340 350
HISDTSVVLS MDNNRSLDLD SIIAEVKAQY EDIAQRSRAE AESWYQTKYE
360 370 380 390 400
ELQVTAGRHG DDLRNTKQEI AEINRMIQRL RSEIDHVKKQ CANLQAAIAD
410 420 430 440 450
AEQRGEMALK DARGKLEGLE DALQKAKQDM ARLLKEYQEL MNVKLALDVE
460 470 480 490 500
IATYRKLLEG EECRLNGEGV GPVNISVVQS TVSSGYGSAG GASSSLGLGG
510 520 530 540 550
GSSYSYSSSH GLGGGFSAGS GRAIGGGLSS SGGLSSSTIK YTTTSSSKKS
553
YRQ

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4031 Collapse assay details

Data source: Panorama

Official Gene Symbol
Krt6a
Peptide Sequence
TAAENEFVTLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
250
Peptide End
260
CPTAC ID
non-CPTAC-4031
Peptide Molecular Mass
1,221.6241
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 5.3 2.5 3 9.6 7.2 5.5 11 7.6 6.3 15 15 15
y7 (1+) 5.5 3.5 1.9 9.3 3.8 3.9 10.8 5.2 4.3 15 15 15
y3 (1+) 7.2 2.9 2.3 11.3 3 3.7 13.4 4.2 4.4 15 15 15
y9 (2+) 7.1 4.1 3.7 10.6 5.9 4.2 12.8 7.2 5.6 15 15 15
b5 (2+) 7.1 6.1 3.9 13.1 7.5 4.7 14.9 9.7 6.1 15 15 15
sum 2 1.2 1.1 9 4.8 3.6 9.2 4.9 3.8 15 15 15


Additional Resources and Comments