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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Itgb2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Itgb2 N/A
Sequence Length (AA) Molecular Weight (Da)
771 85026
Protein Name
Integrin beta-2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MLGLRPSLLL ALAGLFFLGS AVSQECTKYK VSSCRDCIQS GPGCSWCQKL
60 70 80 90 100
NFTGPGEPDS LRCDTRAQLL LKGCPADDIM DPRSIANPEF DQRGQRKQLS
110 120 130 140 150
PQKVTLYLRP GQAAAFNVTF RRAKGYPIDL YYLMDLSYSM LDDLNNVKKL
160 170 180 190 200
GGDLLQALNE ITESGRIGFG SFVDKTVLPF VNTHPEKLRN PCPNKEKACQ
210 220 230 240 250
PPFAFRHVLK LTDNSNQFQT EVGKQLISGN LDAPEGGLDA IMQVAACPEE
260 270 280 290 300
IGWRNVTRLL VFATDDGFHF AGDGKLGAIL TPNDGRCHLE DNMYKRSNEF
310 320 330 340 350
DYPSVGQLAH KLSESNIQPI FAVTKKMVKT YEKLTEIIPK SAVGELSDDS
360 370 380 390 400
SNVVQLIKNA YYKLSSRVFL DHSTLPDTLK VTYDSFCSNG ASSIGKSRGD
410 420 430 440 450
CDGVQINNPV TFQVKVMASE CIQEQSFVIR ALGFTDTVTV QVRPQCECQC
460 470 480 490 500
RDQSREQSLC GGKGVMECGI CRCESGYIGK NCECQTQGRS SQELERNCRK
510 520 530 540 550
DNSSIVCSGL GDCICGQCVC HTSDVPNKEI FGQYCECDNV NCERYNSQVC
560 570 580 590 600
GGSDRGSCNC GKCSCKPGYE GSACQCQRST TGCLNARLVE CSGRGHCQCN
610 620 630 640 650
RCICDEGYQP PMCEDCPSCG SHCRDNHTSC AECLKFDKGP FEKNCSVQCA
660 670 680 690 700
GMTLQTIPLK KKPCKERDSE GCWITYTLQQ KDGRNIYNIH VEDSLECVKG
710 720 730 740 750
PNVAAIVGGT VVGVVLIGVL LLVIWKALTH LTDLREYRRF EKEKLKSQWN
760 770 771
NDNPLFKSAT TTVMNPKFAE S

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4042 Collapse assay details

Data source: Panorama

Official Gene Symbol
Itgb2
Peptide Sequence
SNEFDYPSVGQLAHK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
297
Peptide End
311
CPTAC ID
non-CPTAC-4042
Peptide Molecular Mass
1,690.7951
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y13 (2+) 8.5 7.5 5.4 12.6 6.1 8.8 15.2 9.7 10.3 15 15 15
y12 (2+) 9 6 5.5 8.5 8.7 8.7 12.4 10.6 10.3 15 15 15
y11 (2+) 9.5 6.2 4.9 11.9 9.9 6.7 15.2 11.7 8.3 15 15 15
y9 (2+) 5.8 4.5 6.4 9.8 4.9 6.6 11.4 6.7 9.2 15 15 15
y6 (2+) 47.1 26.6 38.4 52 46.5 38.4 70.2 53.6 54.3 15 15 15
sum 2.7 2.3 3.4 7.4 4.7 5.2 7.9 5.2 6.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments