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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Ocln

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ocln Ocl
Sequence Length (AA) Molecular Weight (Da)
521 59000
Protein Name
Occludin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSVRPFESPP PYRPDEFKPN HYAPSNDMYG GEMHVRPMLS QPAYSFYPED
60 70 80 90 100
EILHFYKWTS PPGVIRILSM LIIVMCIAIF ACVASTLAWD RGYGTGLFGG
110 120 130 140 150
SLNYPYSGFG YGGGYGGGYG GYGYGYGGYT DPRAAKGFLL AMAAFCFIAS
160 170 180 190 200
LVIFVTSVIR SGMSRTRRYY LIVIIVSAIL GIMVFIATIV YIMGVNPTAQ
210 220 230 240 250
ASGSMYGSQI YMICNQFYTP GGTGLYVDQY LYHYCVVDPQ EAIAIVLGFM
260 270 280 290 300
IIVAFALIIF FAVKTRRKMD RYDKSNILWD KEHIYDEQPP NVEEWVKNVS
310 320 330 340 350
AGTQDMPPPP SDYAERVDSP MAYSSNGKVN GKRSYPESFY KSTPLVPEVA
360 370 380 390 400
QEIPLTLSVD DFRQPRYSSN GNLETPSKRA PTKGKAGKGK RTDPDHYETD
410 420 430 440 450
YTTGGESCEE LEEDWVREYP PITSDQQRQL YKRNFDAGLQ EYKSLQAELD
460 470 480 490 500
DVNKELSRLD KELDDYREES EEYMAAADEY NRLKQVKGSA DYKSKRNYCK
510 520 521
QLKSKLSHIK RMVGDYDRRK P

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4054 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ocln
Peptide Sequence
EHIYDEQPPNVEEWVK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
282
Peptide End
297
CPTAC ID
non-CPTAC-4054
Peptide Molecular Mass
2,010.9323
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 8.4 6.9 3.7 8.9 9 5.2 12.2 11.3 6.4 15 15 15
b2 (1+) 30.7 8.8 5.4 29.6 8.7 6.1 42.6 12.4 8.1 15 15 15
b3 (1+) 27.5 8.8 9.8 30.2 9.4 10.3 40.8 12.9 14.2 15 15 15
b7 (1+) 8.3 3.7 6.3 10.4 9.3 7.4 13.3 10 9.7 15 15 15
b9 (2+) 112 107.8 77.1 163.3 157.3 164.5 198 190.7 181.7 15 15 15
sum 7.3 5.2 2.9 8.4 7.9 4.8 11.1 9.5 5.6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments