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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Rai1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Rai1 Kiaa1820
Sequence Length (AA) Molecular Weight (Da)
1889 201571
Protein Name
Retinoic acid-induced protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MQSFRERCGF HGKQQNYPQT SQETSRLENY RQPGQAGLSC DRQRLLAKDY
60 70 80 90 100
YSPQPYTGYE GGTGTPSGTV ATAAADKYHR GSKSLQGRPA FPSYVQDSSP
110 120 130 140 150
YPGRYSGEEG LQTWGGPQPP PPQPQPLPGA VSKYEENLMK KTVVPPPNRQ
160 170 180 190 200
YPEQGPQLPF RTHSLHVPPP QPQQPLAYPK LQRQKPQNDL ASPLPFPQGS
210 220 230 240 250
HFPQHSQSFP TSSTYAPTVQ GGGQGAHSYK SCTAPSAQPH DRPMSANANL
260 270 280 290 300
APGQRVQNLH AYQPGRLGYE QQQQALQGRH HTQETLHYQN LAKYQHYGQQ
310 320 330 340 350
GQGYCPPDTA VRTPEQYYQT FSPSSSHSPA RSVGRSPSYS STPSPLMPNL
360 370 380 390 400
ENFPYSQQPL STGAFPTGIT DHSHFMPLLN PSPTDAASSV DPQAGNCKPL
410 420 430 440 450
QKEKLPDNLL SDLSLQSLTA LTSQVENISN TVQQLLLSKA TMPQKKGVKN
460 470 480 490 500
LVSRTPEQHK SQHCSPEGSG YSAEPAGTPL SEPPSSTPQS THAEPQDTDY
510 520 530 540 550
LSGSEDPLER SFLYCSQARG SPARVNSNSK AKPESVSTCS VTSPDDMSTK
560 570 580 590 600
SDDSFQSLHS TLPLDSFSKF VAGERDCPRL LLSALAQEDL ASEILGLQEA
610 620 630 640 650
IVEKADKAWA EASSLPKDNG KPPFSLENHG ACLDTVAKTS WSQPGEPETL
660 670 680 690 700
PEPLQLDKGG STKDFSPGLF EDPSVAFATT DPKKTSSPLS FGTKPLLGTA
710 720 730 740 750
TPDPTTAAFD CFPDTPTASS VDGANPFAWP EENLGDACPR WGLHPGELTK
760 770 780 790 800
GLEQGAKASD GVGKADAHEA SACMGFQEDH AIGKPAAALS GDFKQQEAEG
810 820 830 840 850
VKEEVGGLLQ CPEVAKANQW LEESRHCCSS TDFGDLPLLP PPGRKEDLEA
860 870 880 890 900
EEEYSSLCEL LGSPEQRPSL QDPLSPKAPL MCTKEEAEEA LDTKAGWVSP
910 920 930 940 950
CHLSGEPAVL LGPSVGAQSK VQSWFESSLS HMKPGEEGPE MERAPGSSGT
960 970 980 990 1000
SQGSLAPKPN KPAVPEGPIA KKEPVPRGKS LRSRRVHRGL PEAEDSPCRV
1010 1020 1030 1040 1050
PALPKDLLLP ESCTGPPQGQ AEGAGAPGRG LSEGLPRMCT RSLTALSEPQ
1060 1070 1080 1090 1100
TPGPPGLTTT PTPPDKLGGK QRAAFKSGKR VGKPSPKAAS SPSNPAALPV
1110 1120 1130 1140 1150
ASDSSPMGSK TKEPDSPSMP GKDQRSMVLR SRTKPQQVFH AKRRRPSESR
1160 1170 1180 1190 1200
IPDCRATKKL PANNHLPTAY KVSSGPQKEG RMNQRVKVPK PGTGNKLSDR
1210 1220 1230 1240 1250
PLHTLKRKSA FMAPVPAKKR SLILRSNNGS GGDGREERAE SSPGLLRRMA
1260 1270 1280 1290 1300
SPQRARPRGS GEPPPPPPLE PPAACMGLST QSSLPSAVRT KVLPPRKGRG
1310 1320 1330 1340 1350
LKLEAIVQKI TSPGLKKLAC RVAGAPPGTP RSPALPERRP GGSPAGAEEG
1360 1370 1380 1390 1400
LGGMGQMLPA ASGADPLCRN PASRSLKGKL LNSKKLSSAA DCPKAEAFMS
1410 1420 1430 1440 1450
PETLPSLGTA RAPKKRSRKG RTGTLGPSKG PLEKRPCPGQ PLLLAPHDRA
1460 1470 1480 1490 1500
SSTQGGGEDN SSGGGKKPKT EELGPASQPP EGRPCQPQTR AQKQPGQASY
1510 1520 1530 1540 1550
SSYSKRKRLS RGRGKTAHAS PCKGRATRRR QQQVLPLDPA EPEIRLKYIS
1560 1570 1580 1590 1600
SCKRLRADSR TPAFSPFVRV EKRDAYTTIC TVVNSPGDEP KPHWKPSSSA
1610 1620 1630 1640 1650
ASSSTSSSSL EPAGASLTTF PGGSVLQQRP SLPLSSTMHL GPVVSKALST
1660 1670 1680 1690 1700
SCLVCCLCQN PANFKDLGDL CGPYYPEHCL PKKKPKLKEK ARLEGTLEEA
1710 1720 1730 1740 1750
SLPLERTLKG LECSASTTAA APTTATITTP TALGRLSRPD GPADPAKQGP
1760 1770 1780 1790 1800
LRTSARGLSR RLQSCYCCDG QGDGGEEVAQ ADKSRKHECS KEAPTEPGGD
1810 1820 1830 1840 1850
TQEHWVHEAC AVWTSGVYLV AGKLFGLQEA MKVAVDMPCT SCHEPGATIS
1860 1870 1880 1889
CSYKGCIHTY HYPCANDTGC TFIEENFTLK CPKHKRLPL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4055 Collapse assay details

Data source: Panorama

Official Gene Symbol
Rai1
Peptide Sequence
HHTQETLHYQNLAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
280
Peptide End
293
CPTAC ID
non-CPTAC-4055
Peptide Molecular Mass
1,718.8489
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b11 (2+) 14.1 12.4 12.1 13.4 20.9 13.4 19.5 24.3 18.1 15 15 15
b7 (2+) 65 49.9 70.2 90.2 93.5 100.2 111.2 106 122.3 15 15 15
b4 (2+) 21.8 12.8 16.3 28 13 15.8 35.5 18.2 22.7 15 15 15
y7 (4+) 106.4 148.1 125.7 175.4 130.9 166.8 205.1 197.7 208.9 15 15 15
y10 (2+) 10.1 11 12.1 14.1 11.7 13.7 17.3 16.1 18.3 15 15 15
sum 9 6 7.1 11.1 7.6 9 14.3 9.7 11.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments