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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Ptprg

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Ptprg N/A
Sequence Length (AA) Molecular Weight (Da)
1442 161243
Protein Name
Receptor-type tyrosine-protein phosphatase gamma
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MRRLLEPCWW ILFLKITSSV LHYVVCFPAL TEGYVGTLQE SRQDSSVQIR
60 70 80 90 100
RRKASGDPYW AYSGAYGPEH WVTSSVSCGG SHQSPIDILD HHARVGDEYQ
110 120 130 140 150
ELQLDGFDNE SSNKTWMKNT GKTVAILLKD DYFVSGAGLP GRFKAEKVEF
160 170 180 190 200
HWGHSNGSAG SEHSVNGRRF PVEMQIFFYN PDDFDSFQTA ISENRIIGAM
210 220 230 240 250
AIFFQVSPRD NSALDPIIHG LKGVVHHEKE TFLDPFILRD LLPASLGSYY
260 270 280 290 300
RYTGSLTTPP CSEIVEWIVF RRPVPISYHQ LEAFYSIFTT EQQDHVKSVE
310 320 330 340 350
YLRNNFRPQQ ALNDRVVSKS AVRDAWNHDL ADFLDNPLGT EASKVCSSPP
360 370 380 390 400
IHMKVQPLNQ TALQVSWSQP ETIYHPPIMN YMISYSWTKN EDEKEKTFTK
410 420 430 440 450
DSDKDLKATI SHVSPDSLYL FRVQAVCRND MRSDFSQTML FQANTTRIFQ
460 470 480 490 500
GTRIVKTGVP TASPASSADM APISSGSSTW TSSGIPFSFV SMATGMGPSS
510 520 530 540 550
SGSQATVASV VTSTLLAGLG FGGGGISSFP STVWPTRLPT ASAASKQAGR
560 570 580 590 600
TVLATTEALA SPGPDVHSAP SKDSEGTEEG EKEEKSESED GEREHEEEEK
610 620 630 640 650
DSEKKEKSEA THTAAESDRT APAPTPSSPH RTAAEGGHQT IPGRRQDHSA
660 670 680 690 700
PATDQPGHVA PDLDPLVDTA TQVPPTATEE HYSGSDPRRP EMPSKKPMSR
710 720 730 740 750
GDRFSEDSKF ITVNPAEKNT SGMLSRPSPG RMEWIIPLIV VSALTFVCLV
760 770 780 790 800
LLIAVLVYWR GCNKIKSKGF PRRSREVPSS GERGEKGSRK CFQTAHFYVE
810 820 830 840 850
DSSSPRVVPN ESVPIIPIPD DMEAIPVKQF GKHIGELYSN SQHGFSEDFE
860 870 880 890 900
EVQRCTADMN ITAEHSNHPD NKHKNRYINI LAYDHSRVKL RPLPGKDSKH
910 920 930 940 950
SDYINANYVD GYNKAKAYIA TQGPLKSTFE DFWRMIWEQN TGIIIMITNL
960 970 980 990 1000
VEKGRRKCDQ YWPTENTEEY GNIIVTLKST KVHACYTVRR LSVRNTKVKK
1010 1020 1030 1040 1050
GQKGNPKGRQ NERTVIQYHY TQWPDMGVPE YALPVLTFVR RSSAARMPDM
1060 1070 1080 1090 1100
GPVLVHCSAG VGRTGTYIVI DSMLQQIKDK STVNVLGFLK HIRTQRNYLV
1110 1120 1130 1140 1150
QTEEQYIFIH DALLEAILGK ETAVSSSQLH SYVNSILIPG VGGKTRLEKQ
1160 1170 1180 1190 1200
FKLITQCNAK YVECFSAQKE CNKEKNRNSS VVPAERARVG LAPLPGMKGT
1210 1220 1230 1240 1250
DYINASYIMG YYRSNEFIIT QHPLPHTTKD FWRMIWDHNA QIIVMLPDNQ
1260 1270 1280 1290 1300
SLAEDEFVYW PSREESMNCE AFTVTLISKD RLCLSNEEQI IIHDFILEAT
1310 1320 1330 1340 1350
QDDYVLEVRH FQCPKWPNPD APISSTFELI NVIKEEALTR DGPTIVHDEY
1360 1370 1380 1390 1400
GAVSAGMLCA LTTLSQQLEN ENAVDVFQVA KMINLMRPGV FTDIEQYQFV
1410 1420 1430 1440 1442
YKAMLSLIST KENGNGPMTG DKNGAVLTAE ESDPAESMES LV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4056 Collapse assay details

Data source: Panorama

Official Gene Symbol
Ptprg
Peptide Sequence
AYIATQGPLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
917
Peptide End
926
CPTAC ID
non-CPTAC-4056
Peptide Molecular Mass
1,060.5917
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 10.2 6.4 6.3 11.9 7.1 5.7 15.7 9.6 8.5 15 15 15
b2 (1+) 6.8 5.7 4.5 7.3 6.8 7 10 8.9 8.3 15 15 15
y4 (1+) 15.7 7.2 7.1 22.2 6.9 7.8 27.2 10 10.5 15 15 15
y7 (1+) 5.5 8.5 4.2 12.4 9.1 8.3 13.6 12.5 9.3 15 15 15
y8 (1+) 6.5 9.8 5.1 12.4 9.1 6.3 14 13.4 8.1 15 15 15
sum 2.7 3.2 2.5 8.1 4.8 5.5 8.5 5.8 6 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments