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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Trpm7

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Trpm7 Chak, Ltrpc7
Sequence Length (AA) Molecular Weight (Da)
1863 212399
Protein Name
Transient receptor potential cation channel subfamily M member 7
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSQKSWIEST LTKRECVYII PSSKDPHRCL PGCQICQQLV RCFCGRLVKQ
60 70 80 90 100
HACFTASLAM KYSDVKLGEH FNQAIEEWSV EKHTEQSPTD AYGVINFQGG
110 120 130 140 150
SHSYRAKYVR LSYDTKPEII LQLLLKEWQM ELPKLVISVH GGMQKFELHP
160 170 180 190 200
RIKQLLGKGL IKAAVTTGAW ILTGGVNTGV AKHVGDALKE HASRSSRKIC
210 220 230 240 250
TIGIAPWGVI ENRNDLVGRD VVAPYQTLLN PLSKLNVLNN LHSHFILVDD
260 270 280 290 300
GTVGKYGAEV RLRRELEKTI NQQRIHARIG QGVPVVALIF EGGPNVILTV
310 320 330 340 350
LEYLQESPPV PVVVCEGTGR AADLLAYIHK QTEEGGNLPD AAEPDIISTI
360 370 380 390 400
KKTFNFGQSE AVHLFQTMME CMKKKELITV FHIGSEDHQD IDVAILTALL
410 420 430 440 450
KGTNASAFDQ LILTLAWDRV DIAKNHVFVY GQQWLVGSLE QAMLDALVMD
460 470 480 490 500
RVSFVKLLIE NGVSMHKFLT IPRLEELYNT KQGPTNPMLF HLIRDVKQGN
510 520 530 540 550
LPPGYKITLI DIGLVIEYLM GGTYRCTYTR KRFRLIYNSL GGNNRRSGRN
560 570 580 590 600
TSSSTPQLRK SHETFGNRAD KKEKMRHNHF IKTAQPYRPK MDASMEEGKK
610 620 630 640 650
KRTKDEIVDI DDPETKRFPY PLNELLIWAC LMKRQVMARF LWQHGEESMA
660 670 680 690 700
KALVACKIYR SMAYEAKQSD LVDDTSEELK QYSNDFGQLA VELLEQSFRQ
710 720 730 740 750
DETMAMKLLT YELKNWSNST CLKLAVSSRL RPFVAHTCTQ MLLSDMWMGR
760 770 780 790 800
LNMRKNSWYK VILSILVPPA ILMLEYKTKA EMSHIPQSQD AHQMTMEDSE
810 820 830 840 850
NNFHNITEEI PMEVFKEVKI LDSSDGKNEM EIHIKSKKLP ITRKFYAFYH
860 870 880 890 900
APIVKFWFNT LAYLGFLMLY TFVVLVKMEQ LPSVQEWIVI AYIFTYAIEK
910 920 930 940 950
VREVFMSEAG KISQKIKVWF SDYFNVSDTI AIISFFVGFG LRFGAKWNYI
960 970 980 990 1000
NAYDNHVFVA GRLIYCLNII FWYVRLLDFL AVNQQAGPYV MMIGKMVANM
1010 1020 1030 1040 1050
FYIVVIMALV LLSFGVPRKA ILYPHEEPSW SLAKDIVFHP YWMIFGEVYA
1060 1070 1080 1090 1100
YEIDVCANDS TLPTICGPGT WLTPFLQAVY LFVQYIIMVN LLIAFFNNVY
1110 1120 1130 1140 1150
LQVKAISNIV WKYQRYHFIM AYHEKPVLPP PLIILSHIVS LFCCVCKRRK
1160 1170 1180 1190 1200
KDKTSDGPKL FLTEEDQKKL HDFEEQCVEM YFDEKDDKFN SGSEERIRVT
1210 1220 1230 1240 1250
FERVEQMSIQ IKEVGDRVNY IKRSLQSLDS QIGHLQDLSA LTVDTLKTLT
1260 1270 1280 1290 1300
AQKASEASKV HNEITRELSI SKHLAQNLID DVPVRPLWKK PSAVNTLSSS
1310 1320 1330 1340 1350
LPQGDRESNN PFLCNIFMKD EKDPQYNLFG QDLPVIPQRK EFNIPEAGSS
1360 1370 1380 1390 1400
CGALFPSAVS PPELRQRRHG VEMLKIFNKN QKLGSSPNSS PHMSSPPTKF
1410 1420 1430 1440 1450
SVSTPSQPSC KSHLESTTKD QEPIFYKAAE GDNIEFGAFV GHRDSMDLQR
1460 1470 1480 1490 1500
FKETSNKIRE LLSNDTPENT LKHVGAAGYS ECCKTSTSLH SVQAESCSRR
1510 1520 1530 1540 1550
ASTEDSPEVD SKAALLPDWL RDRPSNREMP SEGGTLNGLA SPFKPVLDTN
1560 1570 1580 1590 1600
YYYSAVERNN LMRLSQSIPF VPVPPRGEPV TVYRLEESSP SILNNSMSSW
1610 1620 1630 1640 1650
SQLGLCAKIE FLSKEEMGGG LRRAVKVLCT WSEHDILKSG HLYIIKSFLP
1660 1670 1680 1690 1700
EVINTWSSIY KEDTVLHLCL REIQQQRAAQ KLTFAFNQMK PKSIPYSPRF
1710 1720 1730 1740 1750
LEVFLLYCHS AGQWFAVEEC MTGEFRKYNN NNGDEIIPTN TLEEIMLAFS
1760 1770 1780 1790 1800
HWTYEYTRGE LLVLDLQGVG ENLTDPSVIK AEEKRSCDMV FGPANLGEDA
1810 1820 1830 1840 1850
IKNFRAKHHC NSCCRKLKLP DLKRNDYTPD KIIFPQDESS DLNLQSGNST
1860 1863
KESEATNSVR LML

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4068 Collapse assay details

Data source: Panorama

Official Gene Symbol
Trpm7
Peptide Sequence
DQEPIFYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1420
Peptide End
1427
CPTAC ID
non-CPTAC-4068
Peptide Molecular Mass
1,038.5022
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.4 4.1 2.4 7.2 4.8 3.4 9 6.3 4.2 15 15 15
y5 (1+) 6.7 2.6 2.4 6.7 4.6 3.6 9.5 5.3 4.3 15 15 15
y2 (1+) 14.2 5.2 5.5 14.6 6.4 5.1 20.4 8.2 7.5 15 15 15
b2 (1+) 5.1 1.7 3.6 7.7 4 5.8 9.2 4.3 6.8 15 15 15
b3 (1+) 7.3 3.7 2.2 11 4.3 3 13.2 5.7 3.7 15 15 15
sum 3.5 1.2 1.3 6.6 3.7 3.2 7.5 3.9 3.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments