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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Unc45b

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Unc45b Cmya4
Sequence Length (AA) Molecular Weight (Da)
931 103638
Protein Name
Protein unc-45 homolog B
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAEAEAAQLK EEGNRHFQLQ DYKAATKSYS QALKLTKDKA LLATLYRNRA
60 70 80 90 100
ACGLKMESYA QAASDASRAI DINSADIKAL YRRCQALEHL GKLDQAFKDV
110 120 130 140 150
QRCATLEPRN QNFQETLRRL NTSIQEQLRV QFSTDSRVQT MFEILLNENS
160 170 180 190 200
EADKREKAAN NLIVLGREEA GAERIFQSNG VALLLQLMNT QRPELLLAAV
210 220 230 240 250
RTLSGMCSGH RARATAILHA VRIDRICSLM ALENEEMSLA VCNLLQAIID
260 270 280 290 300
SLSGEDKREH RGKEEALVLD TKKDLKQITS HLLDMLVSKK VSGQGRDQAL
310 320 330 340 350
NLLNKNVPRK DLSIHDNSRT IYVVDNGLRK ILKVVGQVPD LPSCLPLTDN
360 370 380 390 400
TRMLASILIN KLYDDLRCDP ERDHFRKICE EYITSKFDPQ DMDKNVNAIQ
410 420 430 440 450
TVSGILQGPF DLGNQLLGMK GVMEMMVALC GSEREADQLV AVEALIHAST
460 470 480 490 500
KLSRATFIIT NGVTLLKQIY KTTKNEKIKI RTLVGLCKLG SAGGSDYGLR
510 520 530 540 550
QFAEGSTEKL AKQCRKWLCN TAIDTRTRRW AVEGLAYLTL DADVKDDFVQ
560 570 580 590 600
DIPALQAMFE LAKARTSDKT ILYSVANTLV NCTNSYDVKE VVPELVQLAK
610 620 630 640 650
FSKQHVPEEH PKDKKDFVDL RVKRLLKAGV ISALACMVKA DSAILTDQTK
660 670 680 690 700
ELLARVFLAL CDNPKDRGTI VAQGGGKALI PLALEGTDVG KVKAAHGLAK
710 720 730 740 750
IAAVSNPDIA FPGERVYEVV RPLVSLLDTQ RDGLQNYEAL LGLTNLSGRS
760 770 780 790 800
DKLRQKIFKE KALPDIENYM FENHDQLRQA ATECMCNMVL NKEVQERFLA
810 820 830 840 850
DGNDRLKLVV LLCGEDDHKL QNAAAGALAM LTAAHKKLCL KMTEVTTQWL
860 870 880 890 900
EILQRLCLHD QLSVQHRGLV IAHNLLSADA ELARKLVESE LLEILTVVGK
910 920 930 931
QEPDEKRAAV VQTARECLIK CMDYGFIKPV S

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4069 Collapse assay details

Data source: Panorama

Official Gene Symbol
Unc45b
Peptide Sequence
HFQLQDYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
16
Peptide End
23
CPTAC ID
non-CPTAC-4069
Peptide Molecular Mass
1,077.5243
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 15.9 6.6 8.1 21.7 6.9 7.9 26.9 9.5 11.3 15 15 15
y7 (1+) 15.8 5.6 4.1 20.6 9.9 4.8 26 11.4 6.3 15 15 15
y2 (1+) 8.8 3.5 5.4 9.3 5.9 6.8 12.8 6.9 8.7 15 15 15
b2 (1+) 7.8 6.4 4.2 9.5 6.6 5.3 12.3 9.2 6.8 15 15 15
b6 (1+) 7.4 4.8 2.7 9.4 6.9 5.2 12 8.4 5.9 15 15 15
sum 3.8 1.6 2.6 8 4.1 4.5 8.9 4.4 5.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments