CDH1, CPTAC-5370 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDH1	CDHE, UVO

Sequence Length (AA)	Molecular Weight (Da)
882	97456

Protein Name
Cadherin-1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MGPWSRSLSA	LLLLLQVSSW	LCQEPEPCHP	GFDAESYTFT	VPRRHLERGR
60	70	80	90	100
VLGRVNFEDC	TGRQRTAYFS	LDTRFKVGTD	GVITVKRPLR	FHNPQIHFLV
110	120	130	140	150
YAWDSTYRKF	STKVTLNTVG	HHHRPPPHQA	SVSGIQAELL	TFPNSSPGLR
160	170	180	190	200
RQKRDWVIPP	ISCPENEKGP	FPKNLVQIKS	NKDKEGKVFY	SITGQGADTP
210	220	230	240	250
PVGVFIIERE	TGWLKVTEPL	DRERIATYTL	FSHAVSSNGN	AVEDPMEILI
260	270	280	290	300
TVTDQNDNKP	EFTQEVFKGS	VMEGALPGTS	VMEVTATDAD	DDVNTYNAAI
310	320	330	340	350
AYTILSQDPE	LPDKNMFTIN	RNTGVISVVT	TGLDRESFPT	YTLVVQAADL
360	370	380	390	400
QGEGLSTTAT	AVITVTDTND	NPPIFNPTTY	KGQVPENEAN	VVITTLKVTD
410	420	430	440	450
ADAPNTPAWE	AVYTILNDDG	GQFVVTTNPV	NNDGILKTAK	GLDFEAKQQY
460	470	480	490	500
ILHVAVTNVV	PFEVSLTTST	ATVTVDVLDV	NEAPIFVPPE	KRVEVSEDFG
510	520	530	540	550
VGQEITSYTA	QEPDTFMEQK	ITYRIWRDTA	NWLEINPDTG	AISTRAELDR
560	570	580	590	600
EDFEHVKNST	YTALIIATDN	GSPVATGTGT	LLLILSDVND	NAPIPEPRTI
610	620	630	640	650
FFCERNPKPQ	VINIIDADLP	PNTSPFTAEL	THGASANWTI	QYNDPTQESI
660	670	680	690	700
ILKPKMALEV	GDYKINLKLM	DNQNKDQVTT	LEVSVCDCEG	AAGVCRKAQP
710	720	730	740	750
VEAGLQIPAI	LGILGGILAL	LILILLLLLF	LRRRAVVKEP	LLPPEDDTRD
760	770	780	790	800
NVYYYDEEGG	GEEDQDFDLS	QLHRGLDARP	EVTRNDVAPT	LMSVPRYLPR
810	820	830	840	850
PANPDEIGNF	IDENLKAADT	DPTAPPYDSL	LVFDYEGSGS	EAASLSSLNS
860	870	880	882
SESDKDQDYD	YLNEWGNRFK	KLADMYGGGE	DD

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-5772 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDH1
Peptide Sequence: GLDARPEVTR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 775
Peptide End: 784

CPTAC ID: CPTAC-5772
Peptide Molecular Mass: 1,112.5938
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: N/A
Matrix: Cell Lysate
Submitting Laboratory: Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI: Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: SCIEX 5500 / Thermo Quantiva
Internal Standard: Stable Isotope Labeled Standard
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Dionex Ultimate 3000
Column Packing: C18 PepMap 100
Column Dimensions: 75 micron ID x 25 cm length
Flow Rate: 300 nL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y5 (1+)	18.2	6.7	2.7	26.6	17.7	8.9	32.2	18.9	9.3	15	15	15
y3 (1+)	18.4	8	2.9	24.1	18.4	9.3	30.3	20.1	9.7	15	15	15
sum	17.5	6.9	2.7	25.6	17.8	9	31	19.1	9.4	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	52.2	11.5	6.7	51	35.1	17.1	73	36.9	18.4	14	14	14
y13 (1+)	35.2	9.3	7.7	53.7	37.9	16.4	64.2	39	18.1	14	14	14
y9 (1+)	44.9	19.1	9.8	60	31	14.2	74.9	36.4	17.3	14	14	14
y5 (1+)	41.9	17.4	8.5	43.6	34.6	21.6	60.5	38.7	23.2	14	14	14
y4 (1+)	32.9	17.8	7	38.3	33.5	18.4	50.5	37.9	19.7	14	14	14
sum	28.9	12.7	6.5	43.9	34.6	17.1	52.6	36.9	18.3	14	14	14

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	32.9	17.8	7	38.3	33.5	18.4	50.5	37.9	19.7	14	14	14
y5 (1+)	41.9	17.4	8.5	43.6	34.6	21.6	60.5	38.7	23.2	14	14	14
y6 (1+)	52.2	11.5	6.7	51	35.1	17.1	73	36.9	18.4	14	14	14
y9 (1+)	44.9	19.1	9.8	60	31	14.2	74.9	36.4	17.3	14	14	14
y13 (1+)	35.2	9.3	7.7	53.7	37.9	16.4	64.2	39	18.1	14	14	14
sum	28.9	12.7	6.5	43.9	34.6	17.1	52.6	36.9	18.3	14	14	14

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b3 (1+)	3.6	1.9	2.2	4.8	2.7	3.4	6	3.3	4	15	15	15
y8 (1+)	5	2.2	1.9	3.6	3.2	2.8	6.2	3.9	3.4	15	15	15
y9 (1+)	2.8	1.6	2.5	4.7	2.4	3.4	5.5	2.9	4.2	15	15	15
y10 (1+)	9.3	4.9	4.8	11.2	5	5.7	14.6	7	7.5	15	15	15
sum	2.9	1.7	1.1	3.4	2.2	2.6	4.5	2.8	2.8	15	15	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y8 (1+)	7.4	4.1	3.6	12.8	5.8	4.3	14.8	7.1	5.6	15	15	15
y9 (1+)	5.2	4.8	3.4	7	4.8	3.9	8.7	6.8	5.2	15	15	15
y10 (1+)	6.7	3.4	5	6.7	3.6	4.4	9.5	5	6.7	15	15	15
y6 (1+)	9.2	5.1	3.9	10.5	6.2	5.2	14	8	6.5	15	15	15
y7 (1+)	3.6	1.7	2.9	4.7	2.9	3.2	5.9	3.4	4.3	15	15	15
sum	2.8	2.3	2.6	3.3	2.8	3	4.3	3.6	4	15	15	15

Search form

CDH1

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5772 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

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Assay Details for CPTAC-5773 Collapse assay details

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Assay Parameters Collapse assay parameters

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Assay Details for non-CPTAC-5542 Collapse assay details

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Assay Parameters Collapse assay parameters

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Assay Details for non-CPTAC-5541 Collapse assay details

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Assay Parameters Collapse assay parameters

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Assay Details for non-CPTAC-5370 Collapse assay details

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Assay Parameters Collapse assay parameters

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Assay Details for non-CPTAC-5367 Collapse assay details

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Assay Parameters Collapse assay parameters

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Assay Details for non-CPTAC-5366 Collapse assay details

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Assay Details for CPTAC-1599 Collapse assay details

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Assay Details for non-CPTAC-3565 Collapse assay details

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