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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

AXL

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
AXL UFO
Sequence Length (AA) Molecular Weight (Da)
894 98336
Protein Name
Tyrosine-protein kinase receptor UFO
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MAWRCPRMGR VPLAWCLALC GWACMAPRGT QAEESPFVGN PGNITGARGL
60 70 80 90 100
TGTLRCQLQV QGEPPEVHWL RDGQILELAD STQTQVPLGE DEQDDWIVVS
110 120 130 140 150
QLRITSLQLS DTGQYQCLVF LGHQTFVSQP GYVGLEGLPY FLEEPEDRTV
160 170 180 190 200
AANTPFNLSC QAQGPPEPVD LLWLQDAVPL ATAPGHGPQR SLHVPGLNKT
210 220 230 240 250
SSFSCEAHNA KGVTTSRTAT ITVLPQQPRN LHLVSRQPTE LEVAWTPGLS
260 270 280 290 300
GIYPLTHCTL QAVLSNDGMG IQAGEPDPPE EPLTSQASVP PHQLRLGSLH
310 320 330 340 350
PHTPYHIRVA CTSSQGPSSW THWLPVETPE GVPLGPPENI SATRNGSQAF
360 370 380 390 400
VHWQEPRAPL QGTLLGYRLA YQGQDTPEVL MDIGLRQEVT LELQGDGSVS
410 420 430 440 450
NLTVCVAAYT AAGDGPWSLP VPLEAWRPGQ AQPVHQLVKE PSTPAFSWPW
460 470 480 490 500
WYVLLGAVVA AACVLILALF LVHRRKKETR YGEVFEPTVE RGELVVRYRV
510 520 530 540 550
RKSYSRRTTE ATLNSLGISE ELKEKLRDVM VDRHKVALGK TLGEGEFGAV
560 570 580 590 600
MEGQLNQDDS ILKVAVKTMK IAICTRSELE DFLSEAVCMK EFDHPNVMRL
610 620 630 640 650
IGVCFQGSER ESFPAPVVIL PFMKHGDLHS FLLYSRLGDQ PVYLPTQMLV
660 670 680 690 700
KFMADIASGM EYLSTKRFIH RDLAARNCML NENMSVCVAD FGLSKKIYNG
710 720 730 740 750
DYYRQGRIAK MPVKWIAIES LADRVYTSKS DVWSFGVTMW EIATRGQTPY
760 770 780 790 800
PGVENSEIYD YLRQGNRLKQ PADCLDGLYA LMSRCWELNP QDRPSFTELR
810 820 830 840 850
EDLENTLKAL PPAQEPDEIL YVNMDEGGGY PEPPGAAGGA DPPTQPDPKD
860 870 880 890 894
SCSCLTAAEV HPAGRYVLCP STTPSPAQPA DRGSPAAPGQ EDGA

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1766 Collapse assay details

Data source: Panorama

Official Gene Symbol
AXL
Peptide Modified Sequence
IYNGDYY[+80.0]R
Modification Type
Phospho (Y)
Protein - Site of Modification
703
Peptide - Site of Modification
7
Peptide Start
697
Peptide End
704
CPTAC ID
CPTAC-1766
Peptide Molecular Mass
1,142.4434
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 5.7 5.3 7.2 7.4 8.8 12 9.3 10.3 14 15 15 15
y4 (1+) 6.5 5.3 8.6 10.9 8.9 11.5 12.7 10.4 14.4 15 15 15
y5 (1+) 4.8 6.5 7.5 8.3 8.3 10.9 9.6 10.5 13.2 15 15 15
y6 (1+) 4.2 6.6 7.7 8.2 8.1 11.2 9.2 10.4 13.6 15 15 15
y7 (1+) 4.5 7.2 9 9.1 8.4 11.9 10.2 11.1 14.9 15 15 15
sum 4.3 6.4 7.5 8.4 8.2 10.8 9.4 10.4 13.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
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Comments

Assay Details for CPTAC-2791 Collapse assay details

Data source: Panorama

Official Gene Symbol
AXL
Peptide Modified Sequence
IYNGDY[+80.0]YR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
697
Peptide End
704
CPTAC ID
CPTAC-2791
Peptide Molecular Mass
1,142.4434
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Mammalian Cell Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
250nl/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 5.5 3.1 2.4 7.2 3.9 3.1 9.1 5 3.9 15 15 15
y2 (1+) 23.8 5.2 5.6 27.9 6.6 5.4 36.7 8.4 7.8 15 15 15
y6 (2+) 27.7 11.4 9.3 37 16.1 9.2 46.2 19.7 13.1 15 15 15
b3 (1+) 35.4 7.7 8.5 32.2 8.6 9 47.9 11.5 12.4 15 15 15
sum 6.5 2.7 2.6 8.5 3.7 3.3 10.7 4.6 4.2 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments