Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ELMO2 CED12A, KIAA1834
Sequence Length (AA) Molecular Weight (Da)
720 82615
Protein Name
Engulfment and cell motility protein 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPPPSDIVKV AIEWPGANAQ LLEIDQKRPL ASIIKEVCDG WSLPNPEYYT
60 70 80 90 100
LRYADGPQLY ITEQTRSDIK NGTILQLAIS PSRAARQLME RTQSSNMETR
110 120 130 140 150
LDAMKELAKL SADVTFATEF INMDGIIVLT RLVESGTKLL SHYSEMLAFT
160 170 180 190 200
LTAFLELMDH GIVSWDMVSI TFIKQIAGYV SQPMVDVSIL QRSLAILESM
210 220 230 240 250
VLNSQSLYQK IAEEITVGQL ISHLQVSNQE IQTYAIALIN ALFLKAPEDK
260 270 280 290 300
RQDMANAFAQ KHLRSIILNH VIRGNRPIKT EMAHQLYVLQ VLTFNLLEER
310 320 330 340 350
MMTKMDPNDQ AQRDIIFELR RIAFDAESDP SNAPGSGTEK RKAMYTKDYK
360 370 380 390 400
MLGFTNHINP AMDFTQTPPG MLALDNMLYL AKVHQDTYIR IVLENSSRED
410 420 430 440 450
KHECPFGRSA IELTKMLCEI LQVGELPNEG RNDYHPMFFT HDRAFEELFG
460 470 480 490 500
ICIQLLNKTW KEMRATAEDF NKVMQVVREQ ITRALPSKPN SLDQFKSKLR
510 520 530 540 550
SLSYSEILRL RQSERMSQDD FQSPPIVELR EKIQPEILEL IKQQRLNRLC
560 570 580 590 600
EGSSFRKIGN RRRQERFWYC RLALNHKVLH YGDLDDNPQG EVTFESLQEK
610 620 630 640 650
IPVADIKAIV TGKDCPHMKE KSALKQNKEV LELAFSILYD PDETLNFIAP
660 670 680 690 700
NKYEYCIWID GLSALLGKDM SSELTKSDLD TLLSMEMKLR LLDLENIQIP
710 720
EAPPPIPKEP SSYDFVYHYG

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1765 Collapse assay details

Data source: Panorama

Official Gene Symbol
ELMO2
Peptide Sequence
LLDLENIQIPEAPPPIPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
691
Peptide End
708
CPTAC ID
CPTAC-1765
Peptide Molecular Mass
1,996.1245
Species
Homo sapiens (Human)
Assay Type
Fractionation PRISM-SRM
Fractionation Approach
high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 (uL/min)

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-PRISMSRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 5.2 2.9 2 4 2.3 4.2 6.6 3.7 4.7 15 15 15
y9 (1+) 3.2 3.1 2.3 3 3 2.3 4.4 4.3 3.3 15 15 15
y10 (1+) 7 3 4 8.4 3.6 4.2 10.9 4.7 5.8 15 15 15
sum 2.5 2.2 1.3 2.8 1.8 1.8 3.8 2.8 2.2 15 15 15


Additional Resources and Comments