Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ATF3 N/A
Sequence Length (AA) Molecular Weight (Da)
181 20576
Protein Name
Cyclic AMP-dependent transcription factor ATF-3
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MMLQHPGQVS ASEVSASAIV PCLSPPGSLV FEDFANLTPF VKEELRFAIQ
60 70 80 90 100
NKHLCHRMSS ALESVTVSDR PLGVSITKAE VAPEEDERKK RRRERNKIAA
110 120 130 140 150
AKCRNKKKEK TECLQKESEK LESVNAELKA QIEELKNEKQ HLIYMLNLHR
160 170 180 181
PTCIVRAQNG RTPEDERNLF IQQIKEGTLQ S

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for CPTAC-1755 Collapse assay details

Data source: Panorama

Official Gene Symbol
ATF3
Peptide Sequence
NLFIQQIK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
168
Peptide End
175
CPTAC ID
CPTAC-1755
Peptide Molecular Mass
1,002.5862
Species
Homo sapiens (Human)
Assay Type
Fractionation PRISM-SRM
Fractionation Approach
high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 (uL/min)

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-PRISMSRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b3 (1+) 2.9 4.1 1.3 4.7 4.4 3.1 5.5 6 3.4 15 15 15
y5 (1+) 3.2 4.1 1.7 4.9 5.1 2.3 5.9 6.5 2.9 15 15 15
y6 (1+) 2.6 4.2 1.5 3 4.9 1.8 4 6.5 2.3 15 15 15
sum 2.1 3.4 1.1 3.3 4 1.9 3.9 5.2 2.2 15 15 15


Additional Resources and Comments