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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

ELMO1

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
ELMO1 KIAA0281
Sequence Length (AA) Molecular Weight (Da)
727 83829
Protein Name
Engulfment and cell motility protein 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPPPADIVKV AIEWPGAYPK LMEIDQKKPL SAIIKEVCDG WSLANHEYFA
60 70 80 90 100
LQHADSSNFY ITEKNRNEIK NGTILRLTTS PAQNAQQLHE RIQSSSMDAK
110 120 130 140 150
LEALKDLASL SRDVTFAQEF INLDGISLLT QMVESGTERY QKLQKIMKPC
160 170 180 190 200
FGDMLSFTLT AFVELMDHGI VSWDTFSVAF IKKIASFVNK SAIDISILQR
210 220 230 240 250
SLAILESMVL NSHDLYQKVA QEITIGQLIP HLQGSDQEIQ TYTIAVINAL
260 270 280 290 300
FLKAPDERRQ EMANILAQKQ LRSIILTHVI RAQRAINNEM AHQLYVLQVL
310 320 330 340 350
TFNLLEDRMM TKMDPQDQAQ RDIIFELRRI AFDAESEPNN SSGSMEKRKS
360 370 380 390 400
MYTRDYKKLG FINHVNPAMD FTQTPPGMLA LDNMLYFAKH HQDAYIRIVL
410 420 430 440 450
ENSSREDKHE CPFGRSSIEL TKMLCEILKV GELPSETCND FHPMFFTHDR
460 470 480 490 500
SFEEFFCICI QLLNKTWKEM RATSEDFNKV MQVVKEQVMR ALTTKPSSLD
510 520 530 540 550
QFKSKLQNLS YTEILKIRQS ERMNQEDFQS RPILELKEKI QPEILELIKQ
560 570 580 590 600
QRLNRLVEGT CFRKLNARRR QDKFWYCRLS PNHKVLHYGD LEESPQGEVP
610 620 630 640 650
HDSLQDKLPV ADIKAVVTGK DCPHMKEKGA LKQNKEVLEL AFSILYDSNC
660 670 680 690 700
QLNFIAPDKH EYCIWTDGLN ALLGKDMMSD LTRNDLDTLL SMEIKLRLLD
710 720 727
LENIQIPDAP PPIPKEPSNY DFVYDCN

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1759 Collapse assay details

Data source: Panorama

Official Gene Symbol
ELMO1
Peptide Sequence
LLDLENIQIPDAPPPIPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
698
Peptide End
715
CPTAC ID
CPTAC-1759
Peptide Molecular Mass
1,982.1088
Species
Homo sapiens (Human)
Assay Type
Fractionation PRISM-SRM
Fractionation Approach
high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 (uL/min)

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-PRISMSRM

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 4 2.7 3.4 4 4 9.8 5.7 4.8 10.4 15 15 15
y7 (1+) 3.5 2.9 4.8 4.8 2.2 4.2 5.9 3.6 6.4 15 15 15
y9 (1+) 3.1 3 2.8 2.7 3 3.2 4.1 4.2 4.3 15 15 15
sum 2.2 2 1.9 2 2.1 4.3 3 2.9 4.7 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments