AMPD1, CPTAC-1753 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
AMPD1	N/A

Sequence Length (AA)	Molecular Weight (Da)
780	90219

Protein Name
AMP deaminase 1

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MNVRIFYSVS	QSPHSLLSLL	FYCAILESRI	SATMPLFKLP	AEEKQIDDAM
60	70	80	90	100
RNFAEKVFAS	EVKDEGGRQE	ISPFDVDEIC	PISHHEMQAH	IFHLETLSTS
110	120	130	140	150
TEARRKKRFQ	GRKTVNLSIP	LSETSSTKLS	HIDEYISSSP	TYQTVPDFQR
160	170	180	190	200
VQITGDYASG	VTVEDFEIVC	KGLYRALCIR	EKYMQKSFQR	FPKTPSKYLR
210	220	230	240	250
NIDGEAWVAN	ESFYPVFTPP	VKKGEDPFRT	DNLPENLGYH	LKMKDGVVYV
260	270	280	290	300
YPNEAAVSKD	EPKPLPYPNL	DTFLDDMNFL	LALIAQGPVK	TYTHRRLKFL
310	320	330	340	350
SSKFQVHQML	NEMDELKELK	NNPHRDFYNC	RKVDTHIHAA	ACMNQKHLLR
360	370	380	390	400
FIKKSYQIDA	DRVVYSTKEK	NLTLKELFAK	LKMHPYDLTV	DSLDVHAGRQ
410	420	430	440	450
TFQRFDKFND	KYNPVGASEL	RDLYLKTDNY	INGEYFATII	KEVGADLVEA
460	470	480	490	500
KYQHAEPRLS	IYGRSPDEWS	KLSSWFVCNR	IHCPNMTWMI	QVPRIYDVFR
510	520	530	540	550
SKNFLPHFGK	MLENIFMPVF	EATINPQADP	ELSVFLKHIT	GFDSVDDESK
560	570	580	590	600
HSGHMFSSKS	PKPQEWTLEK	NPSYTYYAYY	MYANIMVLNS	LRKERGMNTF
610	620	630	640	650
LFRPHCGEAG	ALTHLMTAFM	IADDISHGLN	LKKSPVLQYL	FFLAQIPIAM
660	670	680	690	700
SPLSNNSLFL	EYAKNPFLDF	LQKGLMISLS	TDDPMQFHFT	KEPLMEEYAI
710	720	730	740	750
AAQVFKLSTC	DMCEVARNSV	LQCGISHEEK	VKFLGDNYLE	EGPAGNDIRR
760	770	780
TNVAQIRMAY	RYETWCYELN	LIAEGLKSTE

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1752 Collapse assay details

Data source: Panorama

Official Gene Symbol: AMPD1
Peptide Sequence: TDNLPENLGYHLK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 230
Peptide End: 242

CPTAC ID: CPTAC-1752
Peptide Molecular Mass: 1,512.7573
Species: Homo sapiens (Human)
Assay Type: Fractionation PRISM-SRM
Fractionation Approach: high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.4 (uL/min)

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-PRISMSRM

CPTAC-1752: AMPD1. TDNLPENLGYHLK	CPTAC-1753: AMPD1. YNPVGASELR	CPTAC-1754: ARID4B. LGGFDNIESGAVWK
CPTAC-1755: ATF3. NLFIQQIK	CPTAC-1756: CTNNA2. IASSEFADDPC[+57.0]SSVK	CPTAC-1757: CTNNA2. WDDSGNDIIVLAK
CPTAC-1758: DUSP1. DGTLALAAGALC[+57.0]R	CPTAC-1759: ELMO1. LLDLENIQIPDAPPPIPK	CPTAC-1760: FOSB. IPYEEGPGPGPLAEVR
CPTAC-1761: LAX1. TDDPGTHVQC[+57.0]VK	CPTAC-1762: RAF1. LVADC[+57.0]VK	CPTAC-1763: SLAMF7. VDFPDGGYSLK
CPTAC-1764: SRF. ALIQTC[+57.0]LNSPDSPPR	CPTAC-1765: ELMO2. LLDLENIQIPEAPPPIPK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (2+)	8.5	5.8	11.1	10.2	7.6	14.7	13.3	9.6	18.4	15	15	15
y3 (1+)	12.2	7.3	10.4	11.1	9.4	14.6	16.5	11.9	17.9	15	15	15
y5 (1+)	5.8	2.3	3.6	5.7	5.3	11.7	8.1	5.8	12.2	15	15	15
sum	4.8	2.7	3.9	6.5	5	9.7	8.1	5.7	10.5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-1753 Collapse assay details

Data source: Panorama

Official Gene Symbol: AMPD1
Peptide Sequence: YNPVGASELR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 412
Peptide End: 421

CPTAC ID: CPTAC-1753
Peptide Molecular Mass: 1,104.5564
Species: Homo sapiens (Human)
Assay Type: Fractionation PRISM-SRM
Fractionation Approach: high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu