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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
LAX1 LAX
Sequence Length (AA) Molecular Weight (Da)
398 44085
Protein Name
Lymphocyte transmembrane adapter 1
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MDGVTPTLST IRGRTLESST LHVTPRSLDR NKDQITNIFS GFAGLLAILL
60 70 80 90 100
VVAVFCILWN WNKRKKRQVP YLRVTVMPLL TLPQTRQRAK NIYDILPWRQ
110 120 130 140 150
EDLGRHESRS MRIFSTESLL SRNSESPEHV PSQAGNAFQE HTAHIHATEY
160 170 180 190 200
AVGIYDNAMV PQMCGNLTPS AHCINVRASR DCASISSEDS HDYVNVPTAE
210 220 230 240 250
EIAETLASTK SPSRNLFVLP STQKLEFTEE RDEGCGDAGD CTSLYSPGAE
260 270 280 290 300
DSDSLSNGEG SSQISNDYVN MTGLDLSAIQ ERQLWVAFQC CRDYENVPAA
310 320 330 340 350
DPSGSQQQAE KDVPSSNIGH VEDKTDDPGT HVQCVKRTFL ASGDYADFQP
360 370 380 390 398
FTQSEDSQMK HREEMSNEDS SDYENVLTAK LGGRDSEQGP GTQLLPDE

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1761 Collapse assay details

Data source: Panorama

Official Gene Symbol
LAX1
Peptide Modified Sequence
TDDPGTHVQC[+57.0]VK
Modification Type
Carbamidomethyl (C)
Protein - Site of Modification
334
Peptide - Site of Modification
10
Peptide Start
325
Peptide End
336
CPTAC ID
CPTAC-1761
Peptide Molecular Mass
1,355.6140
Species
Homo sapiens (Human)
Assay Type
Fractionation PRISM-SRM
Fractionation Approach
high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 (uL/min)

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-PRISMSRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (2+) 5.7 4.1 4.7 7.8 6.9 5.2 9.7 8 7 15 15 15
y4 (1+) 20.1 8.5 7.1 23.4 8.3 7 30.8 11.9 10 15 15 15
y5 (1+) 8.5 7.1 10.6 9.7 9.7 13.1 12.9 12 16.9 15 15 15
sum 4.2 3.4 4.2 6.4 5.8 3.7 7.7 6.7 5.6 15 15 15


Additional Resources and Comments