ARID4B, CPTAC-1754 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ARID4B	BRCAA1, RBBP1L1, RBP1L1, SAP180

Sequence Length (AA)	Molecular Weight (Da)
1312	147809

Protein Name
AT-rich interactive domain-containing protein 4B

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKALDEPPYL	TVGTDVSAKY	RGAFCEAKIK	TAKRLVKVKV	TFRHDSSTVE
60	70	80	90	100
VQDDHIKGPL	KVGAIVEVKN	LDGAYQEAVI	NKLTDASWYT	VVFDDGDEKT
110	120	130	140	150
LRRSSLCLKG	ERHFAESETL	DQLPLTNPEH	FGTPVIGKKT	NRGRRSNHIP
160	170	180	190	200
EEESSSSSSD	EDEDDRKQID	ELLGKVVCVD	YISLDKKKAL	WFPALVVCPD
210	220	230	240	250
CSDEIAVKKD	NILVRSFKDG	KFTSVPRKDV	HEITSDTAPK	PDAVLKQAFE
260	270	280	290	300
QALEFHKSRT	IPANWKTELK	EDSSSSEAEE	EEEEEDDEKE	KEDNSSEEEE
310	320	330	340	350
EIEPFPEERE	NFLQQLYKFM	EDRGTPINKR	PVLGYRNLNL	FKLFRLVHKL
360	370	380	390	400
GGFDNIESGA	VWKQVYQDLG	IPVLNSAAGY	NVKCAYKKYL	YGFEEYCRSA
410	420	430	440	450
NIEFQMALPE	KVVNKQCKEC	ENVKEIKVKE	ENETEIKEIK	MEEERNIIPR
460	470	480	490	500
EEKPIEDEIE	RKENIKPSLG	SKKNLLESIP	THSDQEKEVN	IKKPEDNENL
510	520	530	540	550
DDKDDDTTRV	DESLNIKVEA	EEEKAKSGDE	TNKEEDEDDE	EAEEEEEEEE
560	570	580	590	600
EEEDEDDDDN	NEEEEFECYP	PGMKVQVRYG	RGKNQKMYEA	SIKDSDVEGG
610	620	630	640	650
EVLYLVHYCG	WNVRYDEWIK	ADKIVRPADK	NVPKIKHRKK	IKNKLDKEKD
660	670	680	690	700
KDEKYSPKNC	KLRRLSKPPF	QTNPSPEMVS	KLDLTDAKNS	DTAHIKSIEI
710	720	730	740	750
TSILNGLQAS	ESSAEDSEQE	DERGAQDMDN	NGKEESKIDH	LTNNRNDLIS
760	770	780	790	800
KEEQNSSSLL	EENKVHADLV	ISKPVSKSPE	RLRKDIEVLS	EDTDYEEDEV
810	820	830	840	850
TKKRKDVKKD	TTDKSSKPQI	KRGKRRYCNT	EECLKTGSPG	KKEEKAKNKE
860	870	880	890	900
SLCMENSSNS	SSDEDEEETK	AKMTPTKKYN	GLEEKRKSLR	TTGFYSGFSE
910	920	930	940	950
VAEKRIKLLN	NSDERLQNSR	AKDRKDVWSS	IQGQWPKKTL	KELFSDSDTE
960	970	980	990	1000
AAASPPHPAP	EEGVAEESLQ	TVAEEESCSP	SVELEKPPPV	NVDSKPIEEK
1010	1020	1030	1040	1050
TVEVNDRKAE	FPSSGSNSVL	NTPPTTPESP	SSVTVTEGSR	QQSSVTVSEP
1060	1070	1080	1090	1100
LAPNQEEVRS	IKSETDSTIE	VDSVAGELQD	LQSEGNSSPA	GFDASVSSSS
1110	1120	1130	1140	1150
SNQPEPEHPE	KACTGQKRVK	DAQGGGSSSK	KQKRSHKATV	VNNKKKGKGT
1160	1170	1180	1190	1200
NSSDSEELSA	GESITKSQPV	KSVSTGMKSH	STKSPARTQS	PGKCGKNGDK
1210	1220	1230	1240	1250
DPDLKEPSNR	LPKVYKWSFQ	MSDLENMTSA	ERITILQEKL	QEIRKHYLSL
1260	1270	1280	1290	1300
KSEVASIDRR	RKRLKKKERE	SAATSSSSSS	PSSSSITAAV	MLTLAEPSMS
1310	1312
SASQNGMSVE	CR

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-1754 Collapse assay details

Data source: Panorama

Official Gene Symbol: ARID4B
Peptide Sequence: LGGFDNIESGAVWK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 350
Peptide End: 363

CPTAC ID: CPTAC-1754
Peptide Molecular Mass: 1,491.7358
Species: Homo sapiens (Human)
Assay Type: Fractionation PRISM-SRM
Fractionation Approach: high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.4 (uL/min)

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-PRISMSRM

CPTAC-1752: AMPD1. TDNLPENLGYHLK	CPTAC-1753: AMPD1. YNPVGASELR	CPTAC-1754: ARID4B. LGGFDNIESGAVWK
CPTAC-1755: ATF3. NLFIQQIK	CPTAC-1756: CTNNA2. IASSEFADDPC[+57.0]SSVK	CPTAC-1757: CTNNA2. WDDSGNDIIVLAK
CPTAC-1758: DUSP1. DGTLALAAGALC[+57.0]R	CPTAC-1759: ELMO1. LLDLENIQIPDAPPPIPK	CPTAC-1760: FOSB. IPYEEGPGPGPLAEVR
CPTAC-1761: LAX1. TDDPGTHVQC[+57.0]VK	CPTAC-1762: RAF1. LVADC[+57.0]VK	CPTAC-1763: SLAMF7. VDFPDGGYSLK
CPTAC-1764: SRF. ALIQTC[+57.0]LNSPDSPPR	CPTAC-1765: ELMO2. LLDLENIQIPEAPPPIPK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	21.9	6	9	25.4	9.6	8.6	33.5	11.3	12.4	15	15	15
y6 (1+)	7.2	5.1	4.8	8.5	8	4.9	11.1	9.5	6.9	15	15	15
y7 (1+)	3.6	2.8	3.3	5	6.4	4.4	6.2	7	5.5	15	15	15
sum	3.6	2.5	1.8	3.9	6	2.5	5.3	6.5	3.1	15	15	15

ARID4B

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1754 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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ARID4B

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1754 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments