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Overview Data source: UniProt

Official Gene Symbol Other Aliases
ARID4B BRCAA1, RBBP1L1, RBP1L1, SAP180
Sequence Length (AA) Molecular Weight (Da)
1312 147809
Protein Name
AT-rich interactive domain-containing protein 4B
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MKALDEPPYL TVGTDVSAKY RGAFCEAKIK TAKRLVKVKV TFRHDSSTVE
60 70 80 90 100
VQDDHIKGPL KVGAIVEVKN LDGAYQEAVI NKLTDASWYT VVFDDGDEKT
110 120 130 140 150
LRRSSLCLKG ERHFAESETL DQLPLTNPEH FGTPVIGKKT NRGRRSNHIP
160 170 180 190 200
EEESSSSSSD EDEDDRKQID ELLGKVVCVD YISLDKKKAL WFPALVVCPD
210 220 230 240 250
CSDEIAVKKD NILVRSFKDG KFTSVPRKDV HEITSDTAPK PDAVLKQAFE
260 270 280 290 300
QALEFHKSRT IPANWKTELK EDSSSSEAEE EEEEEDDEKE KEDNSSEEEE
310 320 330 340 350
EIEPFPEERE NFLQQLYKFM EDRGTPINKR PVLGYRNLNL FKLFRLVHKL
360 370 380 390 400
GGFDNIESGA VWKQVYQDLG IPVLNSAAGY NVKCAYKKYL YGFEEYCRSA
410 420 430 440 450
NIEFQMALPE KVVNKQCKEC ENVKEIKVKE ENETEIKEIK MEEERNIIPR
460 470 480 490 500
EEKPIEDEIE RKENIKPSLG SKKNLLESIP THSDQEKEVN IKKPEDNENL
510 520 530 540 550
DDKDDDTTRV DESLNIKVEA EEEKAKSGDE TNKEEDEDDE EAEEEEEEEE
560 570 580 590 600
EEEDEDDDDN NEEEEFECYP PGMKVQVRYG RGKNQKMYEA SIKDSDVEGG
610 620 630 640 650
EVLYLVHYCG WNVRYDEWIK ADKIVRPADK NVPKIKHRKK IKNKLDKEKD
660 670 680 690 700
KDEKYSPKNC KLRRLSKPPF QTNPSPEMVS KLDLTDAKNS DTAHIKSIEI
710 720 730 740 750
TSILNGLQAS ESSAEDSEQE DERGAQDMDN NGKEESKIDH LTNNRNDLIS
760 770 780 790 800
KEEQNSSSLL EENKVHADLV ISKPVSKSPE RLRKDIEVLS EDTDYEEDEV
810 820 830 840 850
TKKRKDVKKD TTDKSSKPQI KRGKRRYCNT EECLKTGSPG KKEEKAKNKE
860 870 880 890 900
SLCMENSSNS SSDEDEEETK AKMTPTKKYN GLEEKRKSLR TTGFYSGFSE
910 920 930 940 950
VAEKRIKLLN NSDERLQNSR AKDRKDVWSS IQGQWPKKTL KELFSDSDTE
960 970 980 990 1000
AAASPPHPAP EEGVAEESLQ TVAEEESCSP SVELEKPPPV NVDSKPIEEK
1010 1020 1030 1040 1050
TVEVNDRKAE FPSSGSNSVL NTPPTTPESP SSVTVTEGSR QQSSVTVSEP
1060 1070 1080 1090 1100
LAPNQEEVRS IKSETDSTIE VDSVAGELQD LQSEGNSSPA GFDASVSSSS
1110 1120 1130 1140 1150
SNQPEPEHPE KACTGQKRVK DAQGGGSSSK KQKRSHKATV VNNKKKGKGT
1160 1170 1180 1190 1200
NSSDSEELSA GESITKSQPV KSVSTGMKSH STKSPARTQS PGKCGKNGDK
1210 1220 1230 1240 1250
DPDLKEPSNR LPKVYKWSFQ MSDLENMTSA ERITILQEKL QEIRKHYLSL
1260 1270 1280 1290 1300
KSEVASIDRR RKRLKKKERE SAATSSSSSS PSSSSITAAV MLTLAEPSMS
1310 1312
SASQNGMSVE CR

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1754 Collapse assay details

Data source: Panorama

Official Gene Symbol
ARID4B
Peptide Sequence
LGGFDNIESGAVWK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
350
Peptide End
363
CPTAC ID
CPTAC-1754
Peptide Molecular Mass
1,491.7358
Species
Homo sapiens (Human)
Assay Type
Fractionation PRISM-SRM
Fractionation Approach
high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix
Ovarian cancer tumor tissue lysate
Submitting Laboratory
Pacific Northwest National Laboratory
Submitting Lab PI
Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Waters nanoACQUITY (Part Number 176016000)
Column Packing
Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions
100 um x 100 mm
Flow Rate
0.4 (uL/min)

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-PRISMSRM


Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 21.9 6 9 25.4 9.6 8.6 33.5 11.3 12.4 15 15 15
y6 (1+) 7.2 5.1 4.8 8.5 8 4.9 11.1 9.5 6.9 15 15 15
y7 (1+) 3.6 2.8 3.3 5 6.4 4.4 6.2 7 5.5 15 15 15
sum 3.6 2.5 1.8 3.9 6 2.5 5.3 6.5 3.1 15 15 15

Additional Resources and Comments