CTNNA2, CPTAC-1756 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

Please include the following statement when referencing the CPTAC Assay Portal

We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CTNNA2	CAPR

Sequence Length (AA)	Molecular Weight (Da)
953	105313

Protein Name
Catenin alpha-2

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MTSATSPIIL	KWDPKSLEIR	TLTVERLLEP	LVTQVTTLVN	TSNKGPSGKK
60	70	80	90	100
KGRSKKAHVL	AASVEQATQN	FLEKGEQIAK	ESQDLKEELV	AAVEDVRKQG
110	120	130	140	150
ETMRIASSEF	ADDPCSSVKR	GTMVRAARAL	LSAVTRLLIL	ADMADVMRLL
160	170	180	190	200
SHLKIVEEAL	EAVKNATNEQ	DLANRFKEFG	KEMVKLNYVA	ARRQQELKDP
210	220	230	240	250
HCRDEMAAAR	GALKKNATML	YTASQAFLRH	PDVAATRANR	DYVFKQVQEA
260	270	280	290	300
IAGISNAAQA	TSPTDEAKGH	TGIGELAAAL	NEFDNKIILD	PMTFSEARFR
310	320	330	340	350
PSLEERLESI	ISGAALMADS	SCTRDDRRER	IVAECNAVRQ	ALQDLLSEYM
360	370	380	390	400
NNTGRKEKGD	PLNIAIDKMT	KKTRDLRRQL	RKAVMDHISD	SFLETNVPLL
410	420	430	440	450
VLIEAAKSGN	EKEVKEYAQV	FREHANKLVE	VANLACSISN	NEEGVKLVRM
460	470	480	490	500
AATQIDSLCP	QVINAALTLA	ARPQSKVAQD	NMDVFKDQWE	KQVRVLTEAV
510	520	530	540	550
DDITSVDDFL	SVSENHILED	VNKCVIALQE	GDVDTLDRTA	GAIRGRAARV
560	570	580	590	600
IHIINAEMEN	YEAGVYTEKV	LEATKLLSET	VMPRFAEQVE	VAIEALSANV
610	620	630	640	650
PQPFEENEFI	DASRLVYDGV	RDIRKAVLMI	RTPEELEDDS	DFEQEDYDVR
660	670	680	690	700
SRTSVQTEDD	QLIAGQSARA	IMAQLPQEEK	AKIAEQVEIF	HQEKSKLDAE
710	720	730	740	750
VAKWDDSGND	IIVLAKQMCM	IMMEMTDFTR	GKGPLKNTSD	VINAAKKIAE
760	770	780	790	800
AGSRMDKLAR	AVADQCPDSA	CKQDLLAYLQ	RIALYCHQLN	ICSKVKAEVQ
810	820	830	840	850
NLGGELIVSG	TGVQSTFTTF	YEVDCDVIDG	GRASQLSTHL	PTCAEGAPIG
860	870	880	890	900
SGSSDSSMLD	SATSLIQAAK	NLMNAVVLTV	KASYVASTKY	QKVYGTAAVN
910	920	930	940	950
SPVVSWKMKA	PEKKPLVKRE	KPEEFQTRVR	RGSQKKHISP	VQALSEFKAM
953
DSF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt

Assay Details for CPTAC-1756 Collapse assay details

Data source: Panorama

Official Gene Symbol: CTNNA2
Peptide Modified Sequence: IASSEFADDPC[+57.0]SSVK
Modification Type: Carbamidomethyl (C)
Protein - Site of Modification: 115
Peptide - Site of Modification: 11
Peptide Start: 105
Peptide End: 119

CPTAC ID: CPTAC-1756
Peptide Molecular Mass: 1,611.7087
Species: Homo sapiens (Human)
Assay Type: Fractionation PRISM-SRM
Fractionation Approach: high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM)
Matrix: Ovarian cancer tumor tissue lysate
Submitting Laboratory: Pacific Northwest National Laboratory
Submitting Lab PI: Tao Liu

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Waters nanoACQUITY (Part Number 176016000)
Column Packing: Waters BEH C18, 1.7 um 130 Å (Part Number 186007485)
Column Dimensions: 100 um x 100 mm
Flow Rate: 0.4 (uL/min)

Assay Multiplexing Expand assay panel

Pacific Northwest National Laboratory-PRISMSRM

CPTAC-1752: AMPD1. TDNLPENLGYHLK	CPTAC-1753: AMPD1. YNPVGASELR	CPTAC-1754: ARID4B. LGGFDNIESGAVWK
CPTAC-1755: ATF3. NLFIQQIK	CPTAC-1756: CTNNA2. IASSEFADDPC[+57.0]SSVK	CPTAC-1757: CTNNA2. WDDSGNDIIVLAK
CPTAC-1758: DUSP1. DGTLALAAGALC[+57.0]R	CPTAC-1759: ELMO1. LLDLENIQIPDAPPPIPK	CPTAC-1760: FOSB. IPYEEGPGPGPLAEVR
CPTAC-1761: LAX1. TDDPGTHVQC[+57.0]VK	CPTAC-1762: RAF1. LVADC[+57.0]VK	CPTAC-1763: SLAMF7. VDFPDGGYSLK
CPTAC-1764: SRF. ALIQTC[+57.0]LNSPDSPPR	CPTAC-1765: ELMO2. LLDLENIQIPEAPPPIPK

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	4.3	4	2.3	4.9	8.3	3.3	6.5	9.2	4	15	15	15
y7 (1+)	5.7	5.9	2.1	5.1	6.3	2.7	7.6	8.6	3.4	15	15	15
y8 (1+)	5.6	2.5	2.4	5.1	4.1	2.6	7.6	4.8	3.5	15	15	15
sum	3.9	3.5	1.3	3.2	6.6	1.8	5	7.5	2.2	15	15	15

CTNNA2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1756 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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CTNNA2

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-1756 Collapse assay details

Assay Parameters Collapse assay parameters

Assay Multiplexing Expand assay panel

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments