BTK, CPTAC-2856 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
BTK	AGMX1, ATK, BPK

Sequence Length (AA)	Molecular Weight (Da)
659	76281

Protein Name
Tyrosine-protein kinase BTK

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MAAVILESIF	LKRSQQKKKT	SPLNFKKRLF	LLTVHKLSYY	EYDFERGRRG
60	70	80	90	100
SKKGSIDVEK	ITCVETVVPE	KNPPPERQIP	RRGEESSEME	QISIIERFPY
110	120	130	140	150
PFQVVYDEGP	LYVFSPTEEL	RKRWIHQLKN	VIRYNSDLVQ	KYHPCFWIDG
160	170	180	190	200
QYLCCSQTAK	NAMGCQILEN	RNGSLKPGSS	HRKTKKPLPP	TPEEDQILKK
210	220	230	240	250
PLPPEPAAAP	VSTSELKKVV	ALYDYMPMNA	NDLQLRKGDE	YFILEESNLP
260	270	280	290	300
WWRARDKNGQ	EGYIPSNYVT	EAEDSIEMYE	WYSKHMTRSQ	AEQLLKQEGK
310	320	330	340	350
EGGFIVRDSS	KAGKYTVSVF	AKSTGDPQGV	IRHYVVCSTP	QSQYYLAEKH
360	370	380	390	400
LFSTIPELIN	YHQHNSAGLI	SRLKYPVSQQ	NKNAPSTAGL	GYGSWEIDPK
410	420	430	440	450
DLTFLKELGT	GQFGVVKYGK	WRGQYDVAIK	MIKEGSMSED	EFIEEAKVMM
460	470	480	490	500
NLSHEKLVQL	YGVCTKQRPI	FIITEYMANG	CLLNYLREMR	HRFQTQQLLE
510	520	530	540	550
MCKDVCEAME	YLESKQFLHR	DLAARNCLVN	DQGVVKVSDF	GLSRYVLDDE
560	570	580	590	600
YTSSVGSKFP	VRWSPPEVLM	YSKFSSKSDI	WAFGVLMWEI	YSLGKMPYER
610	620	630	640	650
FTNSETAEHI	AQGLRLYRPH	LASEKVYTIM	YSCWHEKADE	RPTFKILLSN
659
ILDVMDEES

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-6062 Collapse assay details

Data source: Panorama

Official Gene Symbol: BTK
Peptide Sequence: ELGTGQFGVVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 407
Peptide End: 417

CPTAC ID: CPTAC-6062
Peptide Molecular Mass: 1,133.6081
Species: Human
Assay Type: Enrichment PRM
Enrichment Method: N/A
Matrix: FFPE lung tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 5500
Internal Standard: peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.075 mm
Flow Rate: 0.3 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y3 (1+)	44.7	31.9	20	40.2	42.5	21.2	60.1	53.1	29.1	15	15	15
y4 (1+)	24.9	7.8	14.3	34.1	8.6	13.8	42.2	11.6	19.9	15	15	15
y5 (1+)	24	8.4	11.5	25.6	8.3	11.8	35.1	11.8	16.5	15	15	15
y6 (1+)	23.5	12.9	8	24.3	13.9	10.1	33.8	19	12.9	15	15	15
y7 (1+)	18.3	7	6.9	20.6	6.6	5.7	27.6	9.6	8.9	15	15	15
y8 (1+)	63.5	15.4	43.1	46.6	18.6	44	78.8	24.1	61.6	15	15	15
sum	19.9	5.8	13.9	21.2	6	11.5	29.1	8.3	18	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-2856 Collapse assay details

Data source: Panorama

Official Gene Symbol: BTK
Peptide Sequence: ELGTGQFGVVK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 407
Peptide End: 417

CPTAC ID: CPTAC-2856
Peptide Molecular Mass: 1,133.6081
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend