CDK18, CPTAC-2857 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CDK18	PCTAIRE3, PCTK3

Sequence Length (AA)	Molecular Weight (Da)
472	54180

Protein Name
Cyclin-dependent kinase 18

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MNKMKNFKRR	FSLSVPRTET	IEESLAEFTE	QFNQLHNRRN	ENLQLGPLGR
60	70	80	90	100
DPPQECSTFS	PTDSGEEPGQ	LSPGVQFQRR	QNQRRFSMED	VSKRLSLPMD
110	120	130	140	150
IRLPQEFLQK	LQMESPDLPK	PLSRMSRRAS	LSDIGFGKLE	TYVKLDKLGE
160	170	180	190	200
GTYATVFKGR	SKLTENLVAL	KEIRLEHEEG	APCTAIREVS	LLKNLKHANI
210	220	230	240	250
VTLHDLIHTD	RSLTLVFEYL	DSDLKQYLDH	CGNLMSMHNV	KIFMFQLLRG
260	270	280	290	300
LAYCHHRKIL	HRDLKPQNLL	INERGELKLA	DFGLARAKSV	PTKTYSNEVV
310	320	330	340	350
TLWYRPPDVL	LGSTEYSTPI	DMWGVGCIHY	EMATGRPLFP	GSTVKEELHL
360	370	380	390	400
IFRLLGTPTE	ETWPGVTAFS	EFRTYSFPCY	LPQPLINHAP	RLDTDGIHLL
410	420	430	440	450
SSLLLYESKS	RMSAEAALSH	SYFRSLGERV	HQLEDTASIF	SLKEIQLQKD
460	470	472
PGYRGLAFQQ	PGRGKNRRQS	IF

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-5665 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK18
Peptide Sequence: ASLSDIGFGK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: 3
Peptide Start: 131
Peptide End: 140

CPTAC ID: non-CPTAC-5665
Peptide Molecular Mass: 993.5131
Species: Homo sapiens (Human)
Assay Type: Direct PRM
Matrix: SUM159 tryptic digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Q-Exactive
Internal Standard: 25fmol
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: EASY-nLC 1000
Column Packing: C18
Column Dimensions: 75 µm x 50 cm
Flow Rate: 300nL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	2.1	2.6	6	2.9	2.8	5.4	3.6	3.8	8.1	15	15	15
y4 (1+)	3.1	2.3	5.7	4.3	2.9	6.2	5.3	3.7	8.4	15	15	15
y5 (1+)	2.4	2.4	4.7	3.6	3.3	3.6	4.3	4.1	5.9	15	15	15
sum	1.8	2.3	3.5	3.1	2.5	3.6	3.6	3.4	5	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

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Comments

Assay Details for non-CPTAC-2857 Collapse assay details

Data source: Panorama

Official Gene Symbol: CDK18
Peptide Sequence: LDTDGIHLLSSLLLYESK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 392
Peptide End: 409

CPTAC ID: non-CPTAC-2857
Peptide Molecular Mass: 2,016.0779
Species: Homo Sapiens
Assay Type: Direct PRM
Matrix: Tumor Digest
Submitting Laboratory: Washington University in St. Louis
Submitting Lab PI: Reid Townsend