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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CDK18 PCTAIRE3, PCTK3
Sequence Length (AA) Molecular Weight (Da)
474 54424
Protein Name
Cyclin-dependent kinase 18
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MIMNKMKNFK RRFSLSVPRT ETIEESLAEF TEQFNQLHNR RNENLQLGPL
60 70 80 90 100
GRDPPQECST FSPTDSGEEP GQLSPGVQFQ RRQNQRRFSM EDVSKRLSLP
110 120 130 140 150
MDIRLPQEFL QKLQMESPDL PKPLSRMSRR ASLSDIGFGK LETYVKLDKL
160 170 180 190 200
GEGTYATVFK GRSKLTENLV ALKEIRLEHE EGAPCTAIRE VSLLKNLKHA
210 220 230 240 250
NIVTLHDLIH TDRSLTLVFE YLDSDLKQYL DHCGNLMSMH NVKIFMFQLL
260 270 280 290 300
RGLAYCHHRK ILHRDLKPQN LLINERGELK LADFGLARAK SVPTKTYSNE
310 320 330 340 350
VVTLWYRPPD VLLGSTEYST PIDMWGVGCI HYEMATGRPL FPGSTVKEEL
360 370 380 390 400
HLIFRLLGTP TEETWPGVTA FSEFRTYSFP CYLPQPLINH APRLDTDGIH
410 420 430 440 450
LLSSLLLYES KSRMSAEAAL SHSYFRSLGE RVHQLEDTAS IFSLKEIQLQ
460 470 474
KDPGYRGLAF QQPGRGKNRR QSIF

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-5665 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK18
Peptide Sequence
ASLSDIGFGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
131
Peptide End
140
CPTAC ID
non-CPTAC-5665
Peptide Molecular Mass
993.5131
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
SUM159 tryptic digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Q-Exactive
Internal Standard
25fmol
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC 1000
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 2.1 2.6 6 2.9 2.8 5.4 3.6 3.8 8.1 15 15 15
y4 (1+) 3.1 2.3 5.7 4.3 2.9 6.2 5.3 3.7 8.4 15 15 15
y5 (1+) 2.4 2.4 4.7 3.6 3.3 3.6 4.3 4.1 5.9 15 15 15
sum 1.8 2.3 3.5 3.1 2.5 3.6 3.6 3.4 5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2857 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK18
Peptide Sequence
LDTDGIHLLSSLLLYESK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
392
Peptide End
409
CPTAC ID
non-CPTAC-2857
Peptide Molecular Mass
2,016.0779
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 9.3 4.4 9.9 12.6 4.1 12.1 15.7 6 15.6 15 15 15
y10 (1+) 9.3 4.8 11 13.3 4.7 12.7 16.2 6.7 16.8 15 15 15
y4 (1+) 8.9 3.9 9.5 12.1 3.8 11.3 15 5.4 14.8 15 15 15
sum 9.1 4.2 10 12.6 4 11.9 15.5 5.8 15.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2858 Collapse assay details

Data source: Panorama

Official Gene Symbol
CDK18
Peptide Sequence
LPQEFLQK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
103
Peptide End
110
CPTAC ID
non-CPTAC-2858
Peptide Molecular Mass
1,001.5546
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 8.3 2.3 5.3 9.5 3.2 5.3 12.6 3.9 7.5 15 15 15
b5 (1+) 5.5 1.9 3 6 4.2 3.3 8.1 4.6 4.5 15 15 15
y6 (1+) 5.8 2 4.6 7.6 3.1 4.7 9.6 3.7 6.6 15 15 15
sum 6.1 1.3 4.1 7.2 3 4 9.4 3.3 5.7 15 15 15


Additional Resources and Comments