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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
KRT5 N/A
Sequence Length (AA) Molecular Weight (Da)
590 62378
Protein Name
Keratin, type II cytoskeletal 5
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSRQSSVSFR SGGSRSFSTA SAITPSVSRT SFTSVSRSGG GGGGGFGRVS
60 70 80 90 100
LAGACGVGGY GSRSLYNLGG SKRISISTSG GSFRNRFGAG AGGGYGFGGG
110 120 130 140 150
AGSGFGFGGG AGGGFGLGGG AGFGGGFGGP GFPVCPPGGI QEVTVNQSLL
160 170 180 190 200
TPLNLQIDPS IQRVRTEERE QIKTLNNKFA SFIDKVRFLE QQNKVLDTKW
210 220 230 240 250
TLLQEQGTKT VRQNLEPLFE QYINNLRRQL DSIVGERGRL DSELRNMQDL
260 270 280 290 300
VEDFKNKYED EINKRTTAEN EFVMLKKDVD AAYMNKVELE AKVDALMDEI
310 320 330 340 350
NFMKMFFDAE LSQMQTHVSD TSVVLSMDNN RNLDLDSIIA EVKAQYEEIA
360 370 380 390 400
NRSRTEAESW YQTKYEELQQ TAGRHGDDLR NTKHEISEMN RMIQRLRAEI
410 420 430 440 450
DNVKKQCANL QNAIADAEQR GELALKDARN KLAELEEALQ KAKQDMARLL
460 470 480 490 500
REYQELMNTK LALDVEIATY RKLLEGEECR LSGEGVGPVN ISVVTSSVSS
510 520 530 540 550
GYGSGSGYGG GLGGGLGGGL GGGLAGGSSG SYYSSSSGGV GLGGGLSVGG
560 570 580 590
SGFSASSGRG LGVGFGSGGG SSSSVKFVST TSSSRKSFKS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5861 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT5
Peptide Sequence
AQYEEIANR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
344
Peptide End
352
CPTAC ID
CPTAC-5861
Peptide Molecular Mass
1,092.5200
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Frozen breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 12.6 9.6 8 17.2 7.8 9.2 21.3 12.4 12.2 15 17 15
y6 (1+) 17.9 5.9 8.6 18.5 6.8 8.9 25.7 9 12.4 15 17 15
y5 (1+) 15.6 7.1 7 16.1 7.6 7.1 22.4 10.4 10 15 17 15
y3 (1+) 9.7 7.7 9.2 13 6.4 10.2 16.2 10 13.7 15 17 15
y7 (1+) 15.3 3.9 4.6 12.1 9.7 6 19.5 10.5 7.6 15 17 15
sum 13.7 3.3 4 10.6 6.7 5.2 17.3 7.5 6.6 15 17 15


Additional Resources and Comments


Assay Details for CPTAC-5886 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT5
Peptide Sequence
AQYEEIANR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
344
Peptide End
352
CPTAC ID
CPTAC-5886
Peptide Molecular Mass
1,092.5200
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 13 4.3 6.7 13.4 4 5.8 18.7 5.9 8.9 12 12 12
y6 (1+) 19.1 8.8 7.7 16.9 12 8.7 25.5 14.9 11.6 12 12 12
y5 (1+) 11 5.2 11.2 13.3 9.6 11.7 17.3 10.9 16.2 12 12 12
y4 (1+) 18.5 8.2 9.8 14.2 9.3 10.4 23.3 12.4 14.3 12 12 12
y3 (1+) 21.6 8.5 8.9 21.4 13.5 12.3 30.4 16 15.2 12 12 12
sum 12.8 3.3 4.6 11.6 4.4 4.2 17.3 5.5 6.2 12 12 12


Additional Resources and Comments


Assay Details for CPTAC-5842 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT5
Peptide Sequence
SFSTASAITPSVSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
16
Peptide End
29
CPTAC ID
CPTAC-5842
Peptide Molecular Mass
1,409.7151
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
Frozen breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 21.2 21.4 14.1 41.8 61.2 58.9 46.9 64.8 60.6 15 17 15
y9 (1+) 19.2 15.9 14.8 42.7 65 66.4 46.8 66.9 68 15 17 15
y8 (1+) 21.6 22.8 13.4 48.9 80.2 63.4 53.5 83.4 64.8 15 17 15
y7 (1+) 15.5 22.7 14.7 41.8 64.9 65.8 44.6 68.8 67.4 15 17 15
y5 (1+) 20.7 21.6 12.3 33.8 62.6 53.3 39.6 66.2 54.7 15 17 15
sum 17.3 19 11.4 40.6 64.9 61.4 44.1 67.6 62.4 15 17 15


Additional Resources and Comments


Assay Details for CPTAC-5867 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT5
Peptide Sequence
SFSTASAITPSVSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
16
Peptide End
29
CPTAC ID
CPTAC-5867
Peptide Molecular Mass
1,409.7151
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
peptide immunoaffinity
Matrix
FFPE breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 13.8 11.9 11.7 23.1 20.9 22.1 26.9 24.1 25 12 12 12
y6 (1+) 15.2 10.1 12.7 23.3 19 21.5 27.8 21.5 25 12 12 12
y5 (1+) 16.1 10.3 13.4 23.3 25.3 21.1 28.3 27.3 25 12 12 12
y8 (1+) 13.2 13.9 13.6 24.6 31.7 25.6 27.9 34.6 29 12 12 12
y9 (1+) 14.5 12.3 15.3 18.9 26.7 20.4 23.8 29.4 25.5 12 12 12
sum 9.7 8.6 8.2 20.3 22.9 19.9 22.5 24.5 21.5 12 12 12


Additional Resources and Comments


Assay Details for non-CPTAC-3797 Collapse assay details

Data source: Panorama

Official Gene Symbol
Krt5
Peptide Sequence
YEELQQTAGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
359
Peptide End
368
CPTAC ID
non-CPTAC-3797
Peptide Molecular Mass
1,193.5677
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 11.2 11.2 7.6 12.5 11 8.8 16.8 15.7 11.6 15 15 15
y7 (1+) 11.7 6.8 8 11.7 9.4 9 16.5 11.6 12 15 15 15
y5 (1+) 14.9 12.5 7.1 13.5 12.8 11.1 20.1 17.9 13.2 15 15 15
b2 (1+) 16.7 8.1 10.3 17.9 10.9 10.3 24.5 13.6 14.6 15 15 15
b3 (1+) 12.9 10.5 8.7 13.9 14.2 9.2 19 17.7 12.7 15 15 15
sum 5.8 4.6 4.8 5.6 7.3 5.5 8.1 8.6 7.3 15 15 15


Additional Resources and Comments