KRT5, CPTAC-5886 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
KRT5	N/A

Sequence Length (AA)	Molecular Weight (Da)
590	62378

Protein Name
Keratin, type II cytoskeletal 5

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSRQSSVSFR	SGGSRSFSTA	SAITPSVSRT	SFTSVSRSGG	GGGGGFGRVS
60	70	80	90	100
LAGACGVGGY	GSRSLYNLGG	SKRISISTSG	GSFRNRFGAG	AGGGYGFGGG
110	120	130	140	150
AGSGFGFGGG	AGGGFGLGGG	AGFGGGFGGP	GFPVCPPGGI	QEVTVNQSLL
160	170	180	190	200
TPLNLQIDPS	IQRVRTEERE	QIKTLNNKFA	SFIDKVRFLE	QQNKVLDTKW
210	220	230	240	250
TLLQEQGTKT	VRQNLEPLFE	QYINNLRRQL	DSIVGERGRL	DSELRNMQDL
260	270	280	290	300
VEDFKNKYED	EINKRTTAEN	EFVMLKKDVD	AAYMNKVELE	AKVDALMDEI
310	320	330	340	350
NFMKMFFDAE	LSQMQTHVSD	TSVVLSMDNN	RNLDLDSIIA	EVKAQYEEIA
360	370	380	390	400
NRSRTEAESW	YQTKYEELQQ	TAGRHGDDLR	NTKHEISEMN	RMIQRLRAEI
410	420	430	440	450
DNVKKQCANL	QNAIADAEQR	GELALKDARN	KLAELEEALQ	KAKQDMARLL
460	470	480	490	500
REYQELMNTK	LALDVEIATY	RKLLEGEECR	LSGEGVGPVN	ISVVTSSVSS
510	520	530	540	550
GYGSGSGYGG	GLGGGLGGGL	GGGLAGGSSG	SYYSSSSGGV	GLGGGLSVGG
560	570	580	590
SGFSASSGRG	LGVGFGSGGG	SSSSVKFVST	TSSSRKSFKS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-5861 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRT5
Peptide Sequence: AQYEEIANR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 344
Peptide End: 352

CPTAC ID: CPTAC-5861
Peptide Molecular Mass: 1,092.5200
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: peptide immunoaffinity
Matrix: Frozen breast cancer tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 6500
Internal Standard: peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 10.0 µL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	12.6	9.6	8	17.2	7.8	9.2	21.3	12.4	12.2	15	17	15
y6 (1+)	17.9	5.9	8.6	18.5	6.8	8.9	25.7	9	12.4	15	17	15
y5 (1+)	15.6	7.1	7	16.1	7.6	7.1	22.4	10.4	10	15	17	15
y3 (1+)	9.7	7.7	9.2	13	6.4	10.2	16.2	10	13.7	15	17	15
y7 (1+)	15.3	3.9	4.6	12.1	9.7	6	19.5	10.5	7.6	15	17	15
sum	13.7	3.3	4	10.6	6.7	5.2	17.3	7.5	6.6	15	17	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	21.2	21.4	14.1	41.8	61.2	58.9	46.9	64.8	60.6	15	17	15
y9 (1+)	19.2	15.9	14.8	42.7	65	66.4	46.8	66.9	68	15	17	15
y8 (1+)	21.6	22.8	13.4	48.9	80.2	63.4	53.5	83.4	64.8	15	17	15
y7 (1+)	15.5	22.7	14.7	41.8	64.9	65.8	44.6	68.8	67.4	15	17	15
y5 (1+)	20.7	21.6	12.3	33.8	62.6	53.3	39.6	66.2	54.7	15	17	15
sum	17.3	19	11.4	40.6	64.9	61.4	44.1	67.6	62.4	15	17	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	13.8	11.9	11.7	23.1	20.9	22.1	26.9	24.1	25	12	12	12
y6 (1+)	15.2	10.1	12.7	23.3	19	21.5	27.8	21.5	25	12	12	12
y5 (1+)	16.1	10.3	13.4	23.3	25.3	21.1	28.3	27.3	25	12	12	12
y8 (1+)	13.2	13.9	13.6	24.6	31.7	25.6	27.9	34.6	29	12	12	12
y9 (1+)	14.5	12.3	15.3	18.9	26.7	20.4	23.8	29.4	25.5	12	12	12
sum	9.7	8.6	8.2	20.3	22.9	19.9	22.5	24.5	21.5	12	12	12

Search form

KRT5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5861 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

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Assay Details for CPTAC-5886 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

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Assay Details for CPTAC-5842 Collapse assay details

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Assay Parameters Collapse assay parameters

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Repeatability

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Assay Details for CPTAC-5867 Collapse assay details

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Repeatability

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Assay Details for non-CPTAC-3797 Collapse assay details

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Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

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