Krt5, CPTAC-3797 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Krt5	Krt2-5

Sequence Length (AA)	Molecular Weight (Da)
580	61767

Protein Name
Keratin, type II cytoskeletal 5

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MSRQSSVSFR	SGGSRSFSAA	SAITPSVSRT	SFSSVSRSGG	GGGGRISLGG
60	70	80	90	100
ACGAGGYGSR	SLYNVGGSKR	ISYSSGGGSF	RNQFGAGGFG	FGGGAGSGFG
110	120	130	140	150
FGGGAGSGFG	FGGGAGFGGG	YGGAGFPVCP	PGGIQEVTVN	QNLLTPLNLQ
160	170	180	190	200
IDPTIQRVRT	EEREQIKTLN	NKFASFIDKV	RFLEQQNKVL	DTKWALLQEQ
210	220	230	240	250
GTKTIKQNLD	PLFEQYINNL	RRQLDGVLGE	RGRLDSELRN	MQDLVEDYKN
260	270	280	290	300
KYEDEINKRT	TAENEFVMLK	KDVDAAYMNK	VELEARVDAL	MDEINFMKMF
310	320	330	340	350
FDAELSQMQT	HVSDTSVVLS	MDNNRSLDLD	SIIAEVKAQY	EDIANRSRTE
360	370	380	390	400
AESWYQTKYE	ELQQTAGRHG	DDLRNTKHEI	SEMNRMIQRL	RSEIDNVKKQ
410	420	430	440	450
CANLQNAIAE	AEQRGELALK	DARNKLTELE	EALQKAKQDM	ARLLREYQEL
460	470	480	490	500
MNTKLALDVE	IATYRKLLEG	EECRLSGEGV	GPVNISVVTN	SVSSGYGGGS
510	520	530	540	550
SIGVGSGFGG	GLGSGFAGGL	GPRFTRGGGG	LGLGSGLSVG	GSGFSAGSSQ
560	570	580
GGMSFGSGGG	SGSSVKFVST	TSSSRRSFKS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below

All other points link out to UniProt

Assay Details for CPTAC-5861 Collapse assay details

Data source: Panorama

Official Gene Symbol: KRT5
Peptide Sequence: AQYEEIANR
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 344
Peptide End: 352

CPTAC ID: CPTAC-5861
Peptide Molecular Mass: 1,092.5200
Species: Homo sapiens (Human)
Assay Type: Enrichment MRM
Enrichment Method: IMAC
Matrix: Frozen breast cancer tumor tissue lysate pool
Submitting Laboratory: Fred Hutchinson Cancer Research Center
Submitting Lab PI: Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: QTRAP 6500
Internal Standard: peptide
Peptide Standard Purity: Crude
Peptide Standard Label Type: 13C and 15N at C-terminus R

LC: Eksigent nanoLC 425
Column Packing: 3 µm ChromXP C18EP, 120 Å
Column Dimensions: 150 x 0.3 mm
Flow Rate: 10.0 µL/min

Chromatograms

Data source: Panorama

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y4 (1+)	12.6	9.6	8	17.2	7.8	9.2	21.3	12.4	12.2	15	17	15
y6 (1+)	17.9	5.9	8.6	18.5	6.8	8.9	25.7	9	12.4	15	17	15
y5 (1+)	15.6	7.1	7	16.1	7.6	7.1	22.4	10.4	10	15	17	15
y3 (1+)	9.7	7.7	9.2	13	6.4	10.2	16.2	10	13.7	15	17	15
y7 (1+)	15.3	3.9	4.6	12.1	9.7	6	19.5	10.5	7.6	15	17	15
sum	13.7	3.3	4	10.6	6.7	5.2	17.3	7.5	6.6	15	17	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y6 (1+)	21.2	21.4	14.1	41.8	61.2	58.9	46.9	64.8	60.6	15	17	15
y9 (1+)	19.2	15.9	14.8	42.7	65	66.4	46.8	66.9	68	15	17	15
y8 (1+)	21.6	22.8	13.4	48.9	80.2	63.4	53.5	83.4	64.8	15	17	15
y7 (1+)	15.5	22.7	14.7	41.8	64.9	65.8	44.6	68.8	67.4	15	17	15
y5 (1+)	20.7	21.6	12.3	33.8	62.6	53.3	39.6	66.2	54.7	15	17	15
sum	17.3	19	11.4	40.6	64.9	61.4	44.1	67.6	62.4	15	17	15

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y7 (1+)	13.8	11.9	11.7	23.1	20.9	22.1	26.9	24.1	25	12	12	12
y6 (1+)	15.2	10.1	12.7	23.3	19	21.5	27.8	21.5	25	12	12	12
y5 (1+)	16.1	10.3	13.4	23.3	25.3	21.1	28.3	27.3	25	12	12	12
y8 (1+)	13.2	13.9	13.6	24.6	31.7	25.6	27.9	34.6	29	12	12	12
y9 (1+)	14.5	12.3	15.3	18.9	26.7	20.4	23.8	29.4	25.5	12	12	12
sum	9.7	8.6	8.2	20.3	22.9	19.9	22.5	24.5	21.5	12	12	12

Search form

Krt5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for CPTAC-5861 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

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Assay Details for CPTAC-5886 Collapse assay details

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Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

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Assay Details for CPTAC-5842 Collapse assay details

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Assay Details for CPTAC-5867 Collapse assay details

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Assay Details for non-CPTAC-3797 Collapse assay details

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Assay Parameters Collapse assay parameters

Chromatograms

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Repeatability

Additional Resources and Comments

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