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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
AKT2 N/A
Sequence Length (AA) Molecular Weight (Da)
481 55769
Protein Name
RAC-beta serine/threonine-protein kinase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNEVSVIKEG WLHKRGEYIK TWRPRYFLLK SDGSFIGYKE RPEAPDQTLP
60 70 80 90 100
PLNNFSVAEC QLMKTERPRP NTFVIRCLQW TTVIERTFHV DSPDEREEWM
110 120 130 140 150
RAIQMVANSL KQRAPGEDPM DYKCGSPSDS STTEEMEVAV SKARAKVTMN
160 170 180 190 200
DFDYLKLLGK GTFGKVILVR EKATGRYYAM KILRKEVIIA KDEVAHTVTE
210 220 230 240 250
SRVLQNTRHP FLTALKYAFQ THDRLCFVME YANGGELFFH LSRERVFTEE
260 270 280 290 300
RARFYGAEIV SALEYLHSRD VVYRDIKLEN LMLDKDGHIK ITDFGLCKEG
310 320 330 340 350
ISDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF
360 370 380 390 400
YNQDHERLFE LILMEEIRFP RTLSPEAKSL LAGLLKKDPK QRLGGGPSDA
410 420 430 440 450
KEVMEHRFFL SINWQDVVQK KLLPPFKPQV TSEVDTRYFD DEFTAQSITI
460 470 480 481
TPPDRYDSLG LLELDQRTHF PQFSYSASIR E

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5757 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
LLPPFKPQVTSEVDTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
422
Peptide End
437
CPTAC ID
CPTAC-5757
Peptide Molecular Mass
1,825.9938
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 74.6 36.1 22.7 96.1 55 27.2 121.7 65.8 35.4 14 15 15
y6 (1+) 70.7 26 39.5 77.8 37.8 69.9 105.1 45.9 80.3 14 15 15
sum 65.7 18.5 26.8 71.1 32.3 38.3 96.8 37.2 46.7 14 15 15


Additional Resources and Comments


Assay Details for CPTAC-5801 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
THFPQFSYSASIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
468
Peptide End
480
CPTAC ID
CPTAC-5801
Peptide Molecular Mass
1,539.7470
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 15 5.1 4 14.3 14.1 6.2 20.7 15 7.4 16 15 17
y6 (1+) 16.1 5 4.6 13.7 13.7 6 21.1 14.6 7.6 16 15 17
sum 15.3 4.6 4.1 13.7 13.9 6.1 20.5 14.6 7.3 16 15 17


Additional Resources and Comments


Assay Details for CPTAC-5802 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Modified Sequence
THFPQFS[+79.966331]YSASIR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
468
Peptide End
480
CPTAC ID
CPTAC-5802
Peptide Molecular Mass
1,619.7134
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 27.4 20.1 16.8 26.9 23 22 38.4 30.5 27.7 16 15 16
y5 (1+) 30.3 23.8 20.5 52.5 22.5 28.5 60.6 32.8 35.1 16 15 16
y4 (1+) 24.4 13.7 17.2 21.7 18.6 24.1 32.7 23.1 29.6 16 15 16
sum 15.9 19.1 16.4 21.9 19.8 24.8 27.1 27.5 29.7 16 15 16


Additional Resources and Comments


Assay Details for CPTAC-5803 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
THFPQFSYSASIRE
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
468
Peptide End
481
CPTAC ID
CPTAC-5803
Peptide Molecular Mass
1,668.7896
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 18 6 13.7 22.2 20.3 23 28.6 21.2 26.8 16 15 17
y6 (1+) 13.9 5.8 14.7 15 22.3 23.8 20.5 23 28 16 15 17
sum 14.9 5.4 14.3 16.9 21.6 23.5 22.5 22.3 27.5 16 15 17


Additional Resources and Comments


Assay Details for CPTAC-5804 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Modified Sequence
THFPQFS[+79.966331]YSASIRE
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
468
Peptide End
481
CPTAC ID
CPTAC-5804
Peptide Molecular Mass
1,748.7560
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center, H. Lee Moffitt Cancer Center & Research Institute, Broad Institute
Submitting Lab PI
Amanda Paulovich, John Koomen, Steven A. Carr

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
SCIEX 5500 / Thermo Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N
LC
Dionex Ultimate 3000
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 15.1 12 9.2 17.7 15 9.6 23.3 19.2 13.3 16 15 16
y4 (1+) 13.5 13 11.2 16.9 15.6 14.5 21.6 20.3 18.3 16 15 16
b5 (1+) 14.6 10.6 9.6 13.4 13.7 10.1 19.8 17.3 13.9 16 15 16
sum 13.8 11.9 9.4 15.9 14.8 11 21.1 19 14.5 16 15 16


Additional Resources and Comments


Assay Details for non-CPTAC-5335 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
EGISDGATMK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
299
Peptide End
308
CPTAC ID
non-CPTAC-5335
Peptide Molecular Mass
1,007.4594
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 9.7 3.1 2.8 10.2 4.7 4.2 14.1 5.6 5 15 15 15
y5 (1+) 4.9 2.7 3.2 7.6 2.9 4.3 9 4 5.4 15 15 15
y6 (1+) 7.6 3.5 3.8 9.8 4.8 4.9 12.4 5.9 6.2 15 15 15
y7 (1+) 9.8 2.9 2.4 11.7 4.4 4.5 15.3 5.3 5.1 15 15 15
sum 5.9 1.5 1.6 6.8 3.1 3.7 9 3.4 4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5334 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
FYGAEIVSALEYLHSR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
254
Peptide End
269
CPTAC ID
non-CPTAC-5334
Peptide Molecular Mass
1,853.9312
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y14 (2+) 8.9 7.9 1.8 17.4 17 4 19.5 18.7 4.4 15 15 15
y5 (1+) 10.6 5.1 3.3 20.1 13.1 4.3 22.7 14.1 5.4 15 15 15
y6 (1+) 16.4 10.6 3.8 25.6 21.5 5.7 30.4 24 6.9 15 15 15
y9 (1+) 9.2 10.4 2.3 19.3 22.6 3.1 21.4 24.9 3.9 15 15 15
y10 (1+) 13.7 15.5 3.4 19.6 25.1 5.6 23.9 29.5 6.6 15 15 15
sum 9 7.5 1.3 18.2 17.6 3.6 20.3 19.1 3.8 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5529 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
LLPPFKPQVTSEVDTR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
6
Peptide Start
422
Peptide End
437
CPTAC ID
non-CPTAC-5529
Peptide Molecular Mass
1,825.9938
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Line
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
SIS Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y5 (1+) 85.9 21.6 27.3 100.6 37.7 23.9 132.3 43.4 36.3 14 14 14
y6 (1+) 70.7 26.1 12.5 77.8 38.8 23.2 105.1 46.8 26.4 14 14 14
y7 (1+) 106.2 21.7 26.6 88.7 26.8 21.3 138.4 34.5 34.1 14 14 14
y8 (1+) 120.8 42.6 32.5 140.6 46.4 24.1 185.4 63 40.5 14 14 14
y10 (1+) 74.6 20.2 18.8 96.1 31.4 21.2 121.7 37.3 28.3 14 14 14
sum 43.2 15.3 11.1 50.4 24.8 15.9 66.4 29.1 19.4 14 14 14


Additional Resources and Comments


Assay Details for non-CPTAC-5336 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
SLLAGLLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
379
Peptide End
386
CPTAC ID
non-CPTAC-5336
Peptide Molecular Mass
813.5324
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 8.2 3.4 4.8 10.9 3.4 5.9 13.6 4.8 7.6 15 15 15
y5 (1+) 4.4 4.6 2.8 4.9 4.9 3.3 6.6 6.7 4.3 15 15 15
y6 (1+) 4.1 3.1 1.9 5.4 4.1 2.8 6.8 5.1 3.4 15 15 15
sum 2.8 2.7 1.6 4.3 3.3 2.9 5.1 4.3 3.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5339 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
THFPQFSYSASIR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
468
Peptide End
480
CPTAC ID
non-CPTAC-5339
Peptide Molecular Mass
1,539.7470
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 9.5 3.9 2.6 12.4 3.2 3 15.6 5 4 15 15 15
y5 (1+) 6.8 1.7 2.3 9.3 4 3 11.5 4.3 3.8 15 15 15
y6 (1+) 9 3.7 3.4 8.1 4.5 4.3 12.1 5.8 5.5 15 15 15
sum 5 1.8 2.2 6.9 2.8 2.9 8.5 3.3 3.6 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5717 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Modified Sequence
THFPQFS[+79.966331]YSASIR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
468
Peptide End
480
CPTAC ID
non-CPTAC-5717
Peptide Molecular Mass
1,619.7134
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y11-98 (1+) 10 6.9 5.4 12.1 10 5.7 15.7 12.1 7.9 14 14 14
y10 (1+) 16.8 7.2 3.1 14.9 8.5 4.8 22.5 11.1 5.7 14 14 14
y10-98 (1+) 6.8 3.9 2.5 7.2 3.9 2.9 9.9 5.5 3.8 14 14 14
b3 (1+) 5.5 2.8 2.3 5.8 2.8 2 8 4 3 14 14 14
sum 3.5 1.8 1.7 4.5 2.1 1.6 5.7 2.8 2.3 14 14 14


Additional Resources and Comments


Assay Details for non-CPTAC-5338 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
THFPQFSYSASIRE
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
468
Peptide End
481
CPTAC ID
non-CPTAC-5338
Peptide Molecular Mass
1,668.7896
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 14.2 3.6 3 13.6 3.8 3.7 19.7 5.2 4.8 15 15 15
y5 (1+) 10 5.1 5.6 10.2 4.8 5.8 14.3 7 8.1 15 15 15
y6 (1+) 6 4.3 4.2 7.9 6.4 4.5 9.9 7.7 6.2 15 15 15
y7 (1+) 15.8 6 3.5 19 7.5 4.4 24.7 9.6 5.6 15 15 15
sum 6.4 3 2.8 5.8 4.2 3.4 8.6 5.2 4.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5718 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Modified Sequence
THFPQFS[+79.966331]YSASIRE
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
468
Peptide End
481
CPTAC ID
non-CPTAC-5718
Peptide Molecular Mass
1,748.7560
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y12-98 (1+) 18.2 7.4 5.6 22.6 10.3 7.1 29 12.7 9 13 12 12
y11 (1+) 28.1 12.6 13.6 29.1 12.6 13.2 40.5 17.8 19 13 12 12
y11-98 (1+) 8.4 3.5 6.8 6.8 3.2 7.7 10.8 4.7 10.3 13 12 12
y9-98 (1+) 13.6 25.4 15.1 27.2 26.5 14.7 30.4 36.7 21.1 13 12 12
b3 (1+) 3.3 2.4 2.9 5.4 2.6 3.6 6.3 3.5 4.6 13 12 12
sum 2.5 2.5 3.1 4.3 2.8 3.8 5 3.8 4.9 13 12 12


Additional Resources and Comments


Assay Details for CPTAC-788 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
APGEDPMDYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
114
Peptide End
123
CPTAC ID
CPTAC-788
Peptide Molecular Mass
1,121.4699
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 15.1 10.5 9.5 19 12.6 16.8 24.3 16.4 19.3 15 15 15
y4 (1+) 27 10.4 15.7 28.9 15.4 16.1 39.6 18.6 22.5 15 15 15
y5 (1+) 9.3 6.3 3.1 9.5 7.3 5.6 13.3 9.6 6.4 15 15 15
y6 (1+) 17.8 19.5 9.1 16.6 19.9 9.6 24.3 27.9 13.2 15 15 15
y7 (1+) 39.7 14.8 16.7 39.3 13.2 14.1 55.9 19.8 21.9 15 15 15
y8 (1+) 18.8 9.1 7.7 18.8 10.4 8.5 26.6 13.8 11.5 15 15 15
sum 8.3 4.2 3.2 8.7 5.1 3.1 12 6.6 4.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-789 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
YDSLGLLELDQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
456
Peptide End
467
CPTAC ID
CPTAC-789
Peptide Molecular Mass
1,420.7198
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 12.6 16.4 10.4 14.9 18.4 10.9 19.5 24.6 15.1 15 15 15
y5 (1+) 14.1 8.9 7.7 14.7 11.4 8 20.4 14.5 11.1 15 15 15
y6 (1+) 8.6 8 7.1 6 8.6 7.2 10.5 11.7 10.1 15 15 15
y7 (1+) 19.3 6.6 11.7 19.3 10.6 11.7 27.3 12.5 16.5 15 15 15
y8 (1+) 7.2 4.9 4.1 7.2 4.5 5.5 10.2 6.7 6.9 15 15 15
y9 (1+) 20.8 15.6 15.1 20.1 17.7 16.9 28.9 23.6 22.7 15 15 15
y10 (1+) 13.4 10.1 8.5 15 13.9 8.7 20.1 17.2 12.2 15 15 15
b3 (1+) 23.7 10.2 8.5 20.3 11.7 11.9 31.2 15.5 14.6 15 15 15
sum 3 2.7 2.2 5.1 3 2.9 5.9 4 3.6 15 15 15


Additional Resources and Comments