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Overview Data source: UniProt

Official Gene Symbol Other Aliases
AKT2 N/A
Sequence Length (AA) Molecular Weight (Da)
481 55769
Protein Name
RAC-beta serine/threonine-protein kinase
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MNEVSVIKEG WLHKRGEYIK TWRPRYFLLK SDGSFIGYKE RPEAPDQTLP
60 70 80 90 100
PLNNFSVAEC QLMKTERPRP NTFVIRCLQW TTVIERTFHV DSPDEREEWM
110 120 130 140 150
RAIQMVANSL KQRAPGEDPM DYKCGSPSDS STTEEMEVAV SKARAKVTMN
160 170 180 190 200
DFDYLKLLGK GTFGKVILVR EKATGRYYAM KILRKEVIIA KDEVAHTVTE
210 220 230 240 250
SRVLQNTRHP FLTALKYAFQ THDRLCFVME YANGGELFFH LSRERVFTEE
260 270 280 290 300
RARFYGAEIV SALEYLHSRD VVYRDIKLEN LMLDKDGHIK ITDFGLCKEG
310 320 330 340 350
ISDGATMKTF CGTPEYLAPE VLEDNDYGRA VDWWGLGVVM YEMMCGRLPF
360 370 380 390 400
YNQDHERLFE LILMEEIRFP RTLSPEAKSL LAGLLKKDPK QRLGGGPSDA
410 420 430 440 450
KEVMEHRFFL SINWQDVVQK KLLPPFKPQV TSEVDTRYFD DEFTAQSITI
460 470 480 481
TPPDRYDSLG LLELDQRTHF PQFSYSASIR E

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-788 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
APGEDPMDYK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
114
Peptide End
123
CPTAC ID
CPTAC-788
Peptide Molecular Mass
1,121.4699
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 15.1 10.5 9.5 19 12.6 16.8 24.3 16.4 19.3 15 15 15
y4 (1+) 27 10.4 15.7 28.9 15.4 16.1 39.6 18.6 22.5 15 15 15
y5 (1+) 9.3 6.3 3.1 9.5 7.3 5.6 13.3 9.6 6.4 15 15 15
y6 (1+) 17.8 19.5 9.1 16.6 19.9 9.6 24.3 27.9 13.2 15 15 15
y7 (1+) 39.7 14.8 16.7 39.3 13.2 14.1 55.9 19.8 21.9 15 15 15
y8 (1+) 18.8 9.1 7.7 18.8 10.4 8.5 26.6 13.8 11.5 15 15 15
sum 8.3 4.2 3.2 8.7 5.1 3.1 12 6.6 4.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-789 Collapse assay details

Data source: Panorama

Official Gene Symbol
AKT2
Peptide Sequence
YDSLGLLELDQR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
456
Peptide End
467
CPTAC ID
CPTAC-789
Peptide Molecular Mass
1,420.7198
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters Test button response curves

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 12.6 16.4 10.4 14.9 18.4 10.9 19.5 24.6 15.1 15 15 15
y5 (1+) 14.1 8.9 7.7 14.7 11.4 8 20.4 14.5 11.1 15 15 15
y6 (1+) 8.6 8 7.1 6 8.6 7.2 10.5 11.7 10.1 15 15 15
y7 (1+) 19.3 6.6 11.7 19.3 10.6 11.7 27.3 12.5 16.5 15 15 15
y8 (1+) 7.2 4.9 4.1 7.2 4.5 5.5 10.2 6.7 6.9 15 15 15
y9 (1+) 20.8 15.6 15.1 20.1 17.7 16.9 28.9 23.6 22.7 15 15 15
y10 (1+) 13.4 10.1 8.5 15 13.9 8.7 20.1 17.2 12.2 15 15 15
b3 (1+) 23.7 10.2 8.5 20.3 11.7 11.9 31.2 15.5 14.6 15 15 15
sum 3 2.7 2.2 5.1 3 2.9 5.9 4 3.6 15 15 15

Additional Resources and Comments