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Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK14 CSBP,CSBP1,CSBP2,CSPB1,MXI2,SAPK2A
Sequence Length (AA) Molecular Weight (Da)
360 41293
Protein Name
Mitogen-activated protein kinase 14
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSQERPTFYR QELNKTIWEV PERYQNLSPV GSGAYGSVCA AFDTKTGLRV
60 70 80 90 100
AVKKLSRPFQ SIIHAKRTYR ELRLLKHMKH ENVIGLLDVF TPARSLEEFN
110 120 130 140 150
DVYLVTHLMG ADLNNIVKCQ KLTDDHVQFL IYQILRGLKY IHSADIIHRD
160 170 180 190 200
LKPSNLAVNE DCELKILDFG LARHTDDEMT GYVATRWYRA PEIMLNWMHY
210 220 230 240 250
NQTVDIWSVG CIMAELLTGR TLFPGTDHID QLKLILRLVG TPGAELLKKI
260 270 280 290 300
SSESARNYIQ SLTQMPKMNF ANVFIGANPL AVDLLEKMLV LDSDKRITAA
310 320 330 340 350
QALAHAYFAQ YHDPDDEPVA DPYDQSFESR DLLIDEWKSL TYDEVISFVP
360
PPLDQEEMES

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1325 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Modified Sequence
HTDDEMT[+80.0]GYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
180
Peptide - Site of Modification
7
Peptide Start
174
Peptide End
186
CPTAC ID
CPTAC-1325
Peptide Molecular Mass
1,574.6072
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7-98 (1+) 104.9 74 61.2 98.3 138.9 50 143.8 157.4 79 15 15 14
y4 (1+) 47.2 31.5 6.4 57 31.4 23.4 74 44.5 24.3 15 15 15
b5 (1+) 44.1 63.5 34.1 51 79.3 39.2 67.4 101.6 52 15 15 15
sum 39 26.1 8 50 28.4 22.7 63.4 38.6 24.1 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1355 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Modified Sequence
HTDDEMT[+80.0]GYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
180
Peptide - Site of Modification
7
Peptide Start
174
Peptide End
186
CPTAC ID
CPTAC-1355
Peptide Molecular Mass
1,574.6072
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 7.2 6 4.6 7.3 12.4 13.6 10.3 13.8 14.4 15 15 15
y7-98 (2+) 7.8 5.3 6.5 9.1 11.8 11.7 12 12.9 13.4 15 15 15
y4 (1+) 3.8 2.9 1.9 3.7 10.3 8.7 5.3 10.7 8.9 15 15 15
sum 3.7 3 1.7 3.7 10.3 9.6 5.2 10.7 9.7 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-1356 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Modified Sequence
HTDDEMTGY[+80.0]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
182
Peptide - Site of Modification
9
Peptide Start
174
Peptide End
186
CPTAC ID
CPTAC-1356
Peptide Molecular Mass
1,574.6072
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 7.6 7.1 7 7 8.7 13 10.3 11.2 14.8 15 15 15
y5 (1+) 6 7 6.9 12.2 10 13.4 13.6 12.2 15.1 15 15 15
y6 (1+) 5.6 4.3 3 7.9 8.5 9 9.7 9.5 9.5 15 15 15
sum 5.8 3.2 3.8 8 7.5 10 9.9 8.2 10.7 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-878 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Sequence
IVTAKQGAVNSFYTVSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1
Peptide End
17
CPTAC ID
CPTAC-878
Peptide Molecular Mass
1,811.9781
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 30 18.7 15.6 31.3 14.9 18.1 43.4 23.9 23.9 15 15 15
y4 (1+) 32 21.3 6.4 28.5 19.4 6.5 42.9 28.8 9.1 15 15 15
y5 (1+) 31.4 12.6 16.5 25.5 14.9 19.5 40.5 19.5 25.5 15 15 15
y7 (1+) 34 15.8 16.1 26.1 18.4 23.2 42.9 24.3 28.2 15 15 15
y11 (1+) 35.9 16 18.5 28.4 20.4 15.8 45.8 25.9 24.3 15 15 15
sum 14.9 6 7.6 15.5 6.2 7.2 21.5 8.6 10.5 15 15 15

Additional Resources and Comments


Assay Details for CPTAC-879 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Sequence
NYIQSLTQMPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
257
Peptide End
267
CPTAC ID
CPTAC-879
Peptide Molecular Mass
1,321.6700
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 30.6 12.5 16.8 31.2 12.9 16.3 43.7 18 23.4 15 15 15
y4 (1+) 30.6 13.7 24.5 45.4 12.4 23.7 54.7 18.5 34.1 15 15 15
y5 (1+) 22.3 11.1 8.7 21.7 13.5 9.5 31.1 17.5 12.9 15 15 15
y6 (1+) 24.8 29.1 39.8 29.8 26.3 41.2 38.8 39.2 57.3 15 15 15
y7 (1+) 15.9 11.4 9.4 14.5 10.5 9 21.5 15.5 13 15 15 15
y8 (1+) 17 8.5 7 16.2 10.7 10.6 23.5 13.7 12.7 15 15 15
y9 (1+) 14.8 13 12 17.8 12.5 12.2 23.1 18 17.1 15 15 15
b3 (1+) 20.2 8.9 17.7 18.5 9.9 18.1 27.4 13.3 25.3 15 15 15
sum 8.7 5.4 4.3 8 4 4.8 11.8 6.7 6.4 15 15 15

Additional Resources and Comments