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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
MAPK14 CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A
Sequence Length (AA) Molecular Weight (Da)
360 41293
Protein Name
Mitogen-activated protein kinase 14
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSQERPTFYR QELNKTIWEV PERYQNLSPV GSGAYGSVCA AFDTKTGLRV
60 70 80 90 100
AVKKLSRPFQ SIIHAKRTYR ELRLLKHMKH ENVIGLLDVF TPARSLEEFN
110 120 130 140 150
DVYLVTHLMG ADLNNIVKCQ KLTDDHVQFL IYQILRGLKY IHSADIIHRD
160 170 180 190 200
LKPSNLAVNE DCELKILDFG LARHTDDEMT GYVATRWYRA PEIMLNWMHY
210 220 230 240 250
NQTVDIWSVG CIMAELLTGR TLFPGTDHID QLKLILRLVG TPGAELLKKI
260 270 280 290 300
SSESARNYIQ SLTQMPKMNF ANVFIGANPL AVDLLEKMLV LDSDKRITAA
310 320 330 340 350
QALAHAYFAQ YHDPDDEPVA DPYDQSFESR DLLIDEWKSL TYDEVISFVP
360
PPLDQEEMES

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-1325 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Modified Sequence
HTDDEMT[+80.0]GYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
180
Peptide - Site of Modification
7
Peptide Start
174
Peptide End
186
CPTAC ID
CPTAC-1325
Peptide Molecular Mass
1,574.6072
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Digested Cell Lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Abelin, et al. Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. PMID:26912667


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva TSQ
Internal Standard
medium stable isotope peptide (delta 6 amu)
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 3 um, 200A
Column Dimensions
0.075 x 100 mm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7-98 (1+) 104.9 74 61.2 98.3 138.9 50 143.8 157.4 79 15 15 14
y4 (1+) 47.2 31.5 6.4 57 31.4 23.4 74 44.5 24.3 15 15 15
b5 (1+) 44.1 63.5 34.1 51 79.3 39.2 67.4 101.6 52 15 15 15
sum 39 26.1 8 50 28.4 22.7 63.4 38.6 24.1 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1355 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Modified Sequence
HTDDEMT[+80.0]GYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
180
Peptide - Site of Modification
7
Peptide Start
174
Peptide End
186
CPTAC ID
CPTAC-1355
Peptide Molecular Mass
1,574.6072
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 7.2 6 4.6 7.3 12.4 13.6 10.3 13.8 14.4 15 15 15
y7-98 (2+) 7.8 5.3 6.5 9.1 11.8 11.7 12 12.9 13.4 15 15 15
y4 (1+) 3.8 2.9 1.9 3.7 10.3 8.7 5.3 10.7 8.9 15 15 15
sum 3.7 3 1.7 3.7 10.3 9.6 5.2 10.7 9.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-1356 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Modified Sequence
HTDDEMTGY[+80.0]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
182
Peptide - Site of Modification
9
Peptide Start
174
Peptide End
186
CPTAC ID
CPTAC-1356
Peptide Molecular Mass
1,574.6072
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
digested cell lysate
Submitting Laboratory
Broad Institute
Submitting Lab PI
Steven A. Carr

Publication

View Details (opens in a new window)

Mol Cell Proteomics. 2016 May;15(5):1622-41. doi: 10.1074/mcp.M116.058354. Epub 2016 Feb 24. Reduced-representation Phosphosignatures Measured by Quantitative Targeted MS Capture Cellular States and Enable Large-scale Comparison of Drug-induced Phenotypes. Abelin JG1, Patel J1, Lu X1, Feeney CM1, Fagbami L1, Creech AL1, Hu R1, Lam D1, Davison D1, Pino L1, Qiao JW1, Kuhn E1, Officer A1, Li J2, Abbatiello S1, Subramanian A1, Sidman R2, Snyder E3, Carr SA1, Jaffe JD4.

View Details (opens in a new window)

Mol Cell Proteomics. 2014 Jul;13(7):1690-704. doi: 10.1074/mcp.M113.036392. Epub 2014 Apr 9. Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mertins P1, Yang F2, Liu T2, Mani DR3, Petyuk VA2, Gillette MA3, Clauser KR3, Qiao JW3, Gritsenko MA2, Moore RJ2, Levine DA4, Townsend R5, Erdmann-Gilmore P5, Snider JE5, Davies SR5, Ruggles KV6, Fenyo D6, Kitchens RT5, Li S5, Olvera N4, Dao F4, Rodriguez H7, Chan DW8, Liebler D9, White F10, Rodland KD2, Mills GB11, Smith RD2, Paulovich AG12, Ellis M5, Carr SA1


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Quantiva
Internal Standard
light stable isotope peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy NanoLC1000
Column Packing
Reprosil C18, 1.9um, 200A
Column Dimensions
0.075 x 150 mm
Flow Rate
200 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 7.6 7.1 7 7 8.7 13 10.3 11.2 14.8 15 15 15
y5 (1+) 6 7 6.9 12.2 10 13.4 13.6 12.2 15.1 15 15 15
y6 (1+) 5.6 4.3 3 7.9 8.5 9 9.7 9.5 9.5 15 15 15
sum 5.8 3.2 3.8 8 7.5 10 9.9 8.2 10.7 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5408 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Sequence
HTDDEMTGYVATR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
174
Peptide End
186
CPTAC ID
non-CPTAC-5408
Peptide Molecular Mass
1,494.6409
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
RSLCnano and Quantiva
Internal Standard
Stable Isotope Labeled Standard
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
RSLCnano
Column Packing
C18 PepMap 100
Column Dimensions
75 micron ID x 25 cm length
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 16 6.9 5.1 18.6 7.4 7 24.5 10.1 8.7 15 15 15
b5 (1+) 13.9 7.5 3 17.9 10 3.7 22.7 12.5 4.8 15 15 15
y4 (1+) 13.4 5.4 3.7 13.9 5.5 3.3 19.3 7.7 5 15 15 15
y5 (1+) 12.6 5.3 5.2 14.3 5.7 4.9 19.1 7.8 7.1 15 15 15
y6 (1+) 13.5 4.3 3.1 16.5 5.3 3.9 21.3 6.8 5 15 15 15
sum 8.6 3 2.3 8.3 3.8 2.6 12 4.8 3.5 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5699 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Modified Sequence
HTDDEMT[+79.966331]GY[+79.966331]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
174
Peptide End
186
CPTAC ID
non-CPTAC-5699
Peptide Molecular Mass
1,654.5736
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 49.5 19.2 21.7 50.5 24.3 23.6 70.7 31 32.1 15 15 15
b7-98 (1+) 16.2 24.7 13.8 18.5 21.9 23 24.6 33 26.8 15 15 15
y7-98 (1+) 15.1 13.2 8.1 21.6 12.6 12.5 26.4 18.2 14.9 15 15 15
y6 (1+) 14.3 18.6 7.1 14.5 26.1 8.3 20.4 32 10.9 15 15 15
y4 (1+) 28.3 19.4 7.1 26.5 22.2 12.6 38.8 29.5 14.5 15 15 15
sum 14.3 8.5 7.5 16.6 9.6 11.8 21.9 12.8 14 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5700 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Modified Sequence
HTDDEMTGY[+79.966331]VATR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
174
Peptide End
186
CPTAC ID
non-CPTAC-5700
Peptide Molecular Mass
1,574.6072
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 16.5 5.3 6.3 14.2 9.7 6.8 21.8 11.1 9.3 15 15 15
y11 (1+) 15.3 10.1 4 13.9 10.1 4.4 20.7 14.3 5.9 15 15 15
y6 (1+) 12.8 9.2 8.7 14.5 13.5 7.5 19.3 16.3 11.5 15 15 15
y7 (1+) 14.8 11.1 7.4 16.3 15.1 10.9 22 18.7 13.2 15 15 15
sum 6.1 7.1 5.7 6.9 9.7 6.1 9.2 12 8.3 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-5701 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Modified Sequence
HTDDEMT[+79.966331]GYVATR
Modification Type
Phospho (ST)
Protein - Site of Modification
N/A
Peptide - Site of Modification
3
Peptide Start
174
Peptide End
186
CPTAC ID
non-CPTAC-5701
Peptide Molecular Mass
1,574.6072
Species
Homo sapiens (Human)
Assay Type
Enrichment PRM
Enrichment Method
N/A
Matrix
Cell Lysate
Submitting Laboratory
Moffitt Cancer Center
Submitting Lab PI
John Koomen

Publication

View Details (opens in a new window)

Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Thermo Quantiva
Internal Standard
peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Dionex Ultimate 3000 RSLCnano
Column Packing
Pepmap 100
Column Dimensions
75um x 25cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 12.9 5.7 2.7 13 5.6 2.8 18.3 8 3.9 15 15 15
y8-98 (1+) 12 9.7 7.2 17.2 13.8 12.1 21 16.9 14.1 15 15 15
y9-98 (1+) 12.6 9.8 6.5 13.2 10.3 6.3 18.2 14.2 9.1 15 15 15
y11-98 (1+) 12.7 6.7 3.7 15.7 6.6 3.1 20.2 9.4 4.8 15 15 15
y4 (1+) 25.6 11.6 4.5 22.4 12.6 6.2 34 17.1 7.7 15 15 15
sum 5.3 3.8 1.9 8.2 5.3 2.8 9.8 6.5 3.4 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-3479 Collapse assay details

Data source: Panorama

Official Gene Symbol
Mapk14
Peptide Sequence
YIHSADIIHR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
140
Peptide End
149
CPTAC ID
non-CPTAC-3479
Peptide Molecular Mass
1,223.6411
Species
Mus musculus (Mouse)
Assay Type
Direct PRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b6 (1+) 17.3 11.9 9.6 30.2 13.6 12.7 34.8 18.1 15.9 15 15 15
y8 (3+) 15 9.4 8.3 25.9 8.8 9.8 29.9 12.9 12.8 15 15 15
y8 (2+) 10 9.5 7.3 12 10 9 15.6 13.8 11.6 15 15 15
y2 (1+) 14 10.7 5.7 16.9 14.2 11.7 21.9 17.8 13 15 15 15
y3 (1+) 9.2 7.2 5.6 14.1 9.5 8.1 16.8 11.9 9.8 15 15 15
sum 8.8 8.3 2.9 14.3 8.7 6 16.8 12 6.7 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-878 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Sequence
IVTAKQGAVNSFYTVSK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
1
Peptide End
17
CPTAC ID
CPTAC-878
Peptide Molecular Mass
1,811.9781
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 30 18.7 15.6 31.3 14.9 18.1 43.4 23.9 23.9 15 15 15
y4 (1+) 32 21.3 6.4 28.5 19.4 6.5 42.9 28.8 9.1 15 15 15
y5 (1+) 31.4 12.6 16.5 25.5 14.9 19.5 40.5 19.5 25.5 15 15 15
y7 (1+) 34 15.8 16.1 26.1 18.4 23.2 42.9 24.3 28.2 15 15 15
y11 (1+) 35.9 16 18.5 28.4 20.4 15.8 45.8 25.9 24.3 15 15 15
sum 14.9 6 7.6 15.5 6.2 7.2 21.5 8.6 10.5 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3006 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Sequence
LSRPFQSIIHAK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
55
Peptide End
66
CPTAC ID
CPTAC-3006
Peptide Molecular Mass
1,395.7987
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 8.1 4.9 5.5 9.9 5.6 5.5 12.8 7.4 7.8 15 15 15
b5 (1+) 8.5 3.3 5 8.5 5.2 4.6 12 6.2 6.8 15 15 15
y4 (1+) 6.9 5.4 5.5 7.2 5.5 6 10 7.7 8.1 15 15 15
sum 6.2 4.3 5 6.5 5.3 4.8 9 6.8 6.9 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3005 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Sequence
LVGTPGAELLK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
238
Peptide End
248
CPTAC ID
CPTAC-3005
Peptide Molecular Mass
1,096.6492
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y7 (1+) 11.1 9.7 10 14.5 8.1 10.3 18.3 12.6 14.4 15 15 15
b5 (1+) 13.3 11.3 12.7 15.4 11.4 12.2 20.3 16.1 17.6 15 15 15
y9 (1+) 11.7 9.3 10.7 13.5 7.9 10.2 17.9 12.2 14.8 15 15 15
sum 11.3 9.5 10.1 14 8 10.2 18 12.4 14.4 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-879 Collapse assay details

Data source: Panorama

Official Gene Symbol
MAPK14
Peptide Sequence
NYIQSLTQMPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
257
Peptide End
267
CPTAC ID
CPTAC-879
Peptide Molecular Mass
1,321.6700
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Pooled patient derived xenograft breast tumor digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TripleTOF 5600+ (SCIEX)
Internal Standard
Peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
2DPlus nano-LC (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
200 µm x 15 cm
Flow Rate
800 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y3 (1+) 30.6 12.5 16.8 31.2 12.9 16.3 43.7 18 23.4 15 15 15
y4 (1+) 30.6 13.7 24.5 45.4 12.4 23.7 54.7 18.5 34.1 15 15 15
y5 (1+) 22.3 11.1 8.7 21.7 13.5 9.5 31.1 17.5 12.9 15 15 15
y6 (1+) 24.8 29.1 39.8 29.8 26.3 41.2 38.8 39.2 57.3 15 15 15
y7 (1+) 15.9 11.4 9.4 14.5 10.5 9 21.5 15.5 13 15 15 15
y8 (1+) 17 8.5 7 16.2 10.7 10.6 23.5 13.7 12.7 15 15 15
y9 (1+) 14.8 13 12 17.8 12.5 12.2 23.1 18 17.1 15 15 15
b3 (1+) 20.2 8.9 17.7 18.5 9.9 18.1 27.4 13.3 25.3 15 15 15
sum 8.7 5.4 4.3 8 4 4.8 11.8 6.7 6.4 15 15 15


Additional Resources and Comments