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Overview Data source: UniProt

Official Gene Symbol Other Aliases
KRT10 KPP
Sequence Length (AA) Molecular Weight (Da)
584 58827
Protein Name
Keratin, type I cytoskeletal 10
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSVRYSSSKH YSSSRSGGGG GGGGCGGGGG VSSLRISSSK GSLGGGFSSG
60 70 80 90 100
GFSGGSFSRG SSGGGCFGGS SGGYGGLGGF GGGSFRGSYG SSSFGGSYGG
110 120 130 140 150
IFGGGSFGGG SFGGGSFGGG GFGGGGFGGG FGGGFGGDGG LLSGNEKVTM
160 170 180 190 200
QNLNDRLASY LDKVRALEES NYELEGKIKE WYEKHGNSHQ GEPRDYSKYY
210 220 230 240 250
KTIDDLKNQI LNLTTDNANI LLQIDNARLA ADDFRLKYEN EVALRQSVEA
260 270 280 290 300
DINGLRRVLD ELTLTKADLE MQIESLTEEL AYLKKNHEEE MKDLRNVSTG
310 320 330 340 350
DVNVEMNAAP GVDLTQLLNN MRSQYEQLAE QNRKDAEAWF NEKSKELTTE
360 370 380 390 400
IDNNIEQISS YKSEITELRR NVQALEIELQ SQLALKQSLE ASLAETEGRY
410 420 430 440 450
CVQLSQIQAQ ISALEEQLQQ IRAETECQNT EYQQLLDIKI RLENEIQTYR
460 470 480 490 500
SLLEGEGSSG GGGRGGGSFG GGYGGGSSGG GSSGGGHGGG HGGSSGGGYG
510 520 530 540 550
GGSSGGGSSG GGYGGGSSSG GHGGSSSGGY GGGSSGGGGG GYGGGSSGGG
560 570 580 584
SSSGGGYGGG SSSGGHKSSS SGSVGESSSK GPRY

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-5854 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT10
Peptide Sequence
LAADDFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
229
Peptide End
235
CPTAC ID
CPTAC-5854
Peptide Molecular Mass
806.3923
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
Frozen breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y2 (1+) 29.1 12.8 8.8 32.7 19 11.5 43.8 22.9 14.5 15 17 15
b5 (1+) 50.3 34.6 14.7 64.8 36.5 22.8 82 50.3 27.1 15 17 15
y3 (1+) 47.5 20 12.4 52.5 17.9 14.9 70.8 26.8 19.4 15 17 15
y4 (1+) 47.6 16.5 11.5 50.8 15.9 15 69.6 22.9 18.9 15 17 15
y6 (1+) 36 15.4 7.6 33.9 14.8 13.9 49.4 21.4 15.8 15 17 15
sum 22.5 12.4 7.9 26.7 14.4 12.9 34.9 19 15.1 15 17 15


Additional Resources and Comments


Assay Details for CPTAC-5879 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT10
Peptide Sequence
LAADDFR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
229
Peptide End
235
CPTAC ID
CPTAC-5879
Peptide Molecular Mass
806.3923
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
N/A
Matrix
FFPE breast cancer tumor tissue lysate pool
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Quantification of Human Epidermal Growth Factor Receptor 2 by Immunopeptide Enrichment and Targeted Mass Spectrometry in Formalin-Fixed Paraffin-Embedded and Frozen Breast Cancer Tissues. Kennedy JJ, Whiteaker JR, Kennedy LC, Bosch DE, Lerch ML, Schoenherr RM, Zhao L, Lin C, Chowdhury S, Kilgore MR, Allison KH, Wang P, Hoofnagle AN, Baird GS, Paulovich AG. Clin Chem. 2021 Jun 17:hvab047. doi: 10.1093/clinchem/hvab047. Online ahead of print. PMID: 34136904


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTRAP 6500
Internal Standard
peptide
Peptide Standard Purity
Crude
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Eksigent nanoLC 425
Column Packing
3 µm ChromXP C18EP, 120 Å
Column Dimensions
150 x 0.3 mm
Flow Rate
10.0 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b5 (1+) 52.2 18.8 15.3 50.8 18 8.7 72.8 26 17.6 12 12 12
y2 (1+) 29.8 7.9 2.8 29.1 6.5 3.2 41.7 10.2 4.3 12 12 12
y3 (1+) 74.9 21.1 7.3 72.6 21.4 6.3 104.3 30.1 9.6 12 12 12
y4 (1+) 34.1 18.4 5.5 32.7 23 12.4 47.2 29.5 13.6 12 12 12
y6 (1+) 26 10.7 6.4 27.8 9.2 7.6 38.1 14.1 9.9 12 12 12
sum 21.3 7 3.4 19.9 4.8 4.5 29.1 8.5 5.6 12 12 12


Additional Resources and Comments


Assay Details for non-CPTAC-3987 Collapse assay details

Data source: Panorama

Official Gene Symbol
Krt10
Peptide Sequence
ALEESNYELEGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
164
Peptide End
175
CPTAC ID
non-CPTAC-3987
Peptide Molecular Mass
1,380.6409
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 15.5 6.6 8.1 15.9 7.9 7.2 22.2 10.3 10.8 15 15 15
y2 (1+) 11.8 7.7 5.1 16.1 10.4 6 20 12.9 7.9 15 15 15
y3 (1+) 16 4 5.8 18.6 8.1 7.4 24.5 9 9.4 15 15 15
y6 (1+) 19.8 7 8.6 19.4 10.9 8 27.7 13 11.7 15 15 15
y8 (1+) 10.7 4.6 5 10.8 7.9 7.6 15.2 9.1 9.1 15 15 15
sum 7.4 3.3 3.3 11.8 6.5 4.6 13.9 7.3 5.7 15 15 15


Additional Resources and Comments


Assay Details for non-CPTAC-2680 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT10
Peptide Sequence
SLLEGEGSSGGGGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
451
Peptide End
464
CPTAC ID
non-CPTAC-2680
Peptide Molecular Mass
1,261.5899
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 10.5 4.5 5 15.6 8 7.7 18.8 9.2 9.2 15 15 15
y12 (2+) 17.8 6.1 6.8 22.1 8.8 8.7 28.4 10.7 11 15 15 15
y8 (1+) 10.1 6.3 5.8 14.8 10 8.3 17.9 11.8 10.1 15 15 15
y11 (1+) 12.9 6.8 4.6 19.9 10.4 7.2 23.7 12.4 8.5 15 15 15
y10 (1+) 7.8 3.7 5.8 14.2 8.5 9 16.2 9.3 10.7 15 15 15
sum 5.6 3.3 4 13.6 7.9 7.5 14.7 8.6 8.5 15 15 15


Additional Resources and Comments