Show Nav →

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Krt10 Krt1-10
Sequence Length (AA) Molecular Weight (Da)
570 57770
Protein Name
Keratin, type I cytoskeletal 10
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MSVLYSSSSK QFSSSRSGGG GGGGSVRVSS TRGSLGGGYS SGGFSGGSFS
60 70 80 90 100
RGSSGGGCFG GSSGGYGGFG GGGSFGGGYG GSSFGGGYGG SSFGGGYGGS
110 120 130 140 150
SFGGAGFGGG GSFGGGSFGG GSYGGGFGGG GFGGDGGSLL SGNGRVTMQN
160 170 180 190 200
LNDRLASYMD KVRALEESNY ELEGKIKEWY EKHGNSSQRE PRDYSKYYKT
210 220 230 240 250
IEDLKGQILT LTTDNANVLL QIDNARLAAD DFRLKYENEV TLRQSVEADI
260 270 280 290 300
NGLRRVLDEL TLSKSDLEMQ IESLNEELAY LKKNHEEEMR DLQNVSTGDV
310 320 330 340 350
NVEMNAAPGV DLTQLLNNMR NQYEQLAEKN RKDAEEWFNQ KSKELTTEID
360 370 380 390 400
SNIEQMSSHK SEITELRRTV QGLEIELQSQ LALKQSLEAS LAETEGRYCV
410 420 430 440 450
QLSQIQSQIS ALEEQLQQIR AETECQNAEY QQLLDIKTRL ENEIQTYRSL
460 470 480 490 500
LEGEGSSSGG GGGRRGGSGG GSYGGSSGGG SYGGSSGGGG SYGGSSGGGG
510 520 530 540 550
SYGGGSSGGG SHGGSSGGGY GGGSSSGGAG GHGGSSGGGY GGGSSSGGQG
560 570
GSGGFKSSGG GDQSSKGPRY

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-3987 Collapse assay details

Data source: Panorama

Official Gene Symbol
Krt10
Peptide Sequence
ALEESNYELEGK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
164
Peptide End
175
CPTAC ID
non-CPTAC-3987
Peptide Molecular Mass
1,380.6409
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 15.5 6.6 8.1 15.9 7.9 7.2 22.2 10.3 10.8 15 15 15
y2 (1+) 11.8 7.7 5.1 16.1 10.4 6 20 12.9 7.9 15 15 15
y3 (1+) 16 4 5.8 18.6 8.1 7.4 24.5 9 9.4 15 15 15
y6 (1+) 19.8 7 8.6 19.4 10.9 8 27.7 13 11.7 15 15 15
y8 (1+) 10.7 4.6 5 10.8 7.9 7.6 15.2 9.1 9.1 15 15 15
sum 7.4 3.3 3.3 11.8 6.5 4.6 13.9 7.3 5.7 15 15 15

Additional Resources and Comments


Assay Details for non-CPTAC-2680 Collapse assay details

Data source: Panorama

Official Gene Symbol
KRT10
Peptide Sequence
SLLEGEGSSGGGGR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
451
Peptide End
464
CPTAC ID
non-CPTAC-2680
Peptide Molecular Mass
1,261.5899
Species
Homo sapiens (Human)
Assay Type
Direct MRM
Matrix
plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
6490 Triple Quad (Agilent)
Internal Standard
synthetic peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b2 (1+) 10.5 4.5 5 15.6 8 7.7 18.8 9.2 9.2 15 15 15
y12 (2+) 17.8 6.1 6.8 22.1 8.8 8.7 28.4 10.7 11 15 15 15
y8 (1+) 10.1 6.3 5.8 14.8 10 8.3 17.9 11.8 10.1 15 15 15
y11 (1+) 12.9 6.8 4.6 19.9 10.4 7.2 23.7 12.4 8.5 15 15 15
y10 (1+) 7.8 3.7 5.8 14.2 8.5 9 16.2 9.3 10.7 15 15 15
sum 5.6 3.3 4 13.6 7.9 7.5 14.7 8.6 8.5 15 15 15

Additional Resources and Comments