Show Nav →
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Hrnr

Previous Assay Next Assay
Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
Hrnr N/A
Sequence Length (AA) Molecular Weight (Da)
2496 247587
Protein Name
Hornerin
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPKLLESIVT VIDVFYQYAT EYGNCDMLSK EEMKELLVTE FHQILKNPDD
60 70 80 90 100
PDTVDIIMQN LDRDHNHKVD FTEYLLMILK LTKACNKIIG KDYCQASGSK
110 120 130 140 150
QKNHSHQHQE EQSKKETENK EQKGSISSSA GENDSYSRGS RGSNKSKSKK
160 170 180 190 200
LRKGKEQSSK QTTKSNSSDH ENSEDYEQGQ HESGFSNSSG NGRPSSRKAS
210 220 230 240 250
GFPQPGSEQG QSSSSSTKGS GECYSSGNGK HGSSSGGSAV SGSGHSNTYG
260 270 280 290 300
KQGTGSRHSS SNRRSRSTSR ESSGSQEYSS GSSEEPGFTH GSGRKNSSTC
310 320 330 340 350
GKNGSYSGQS TGRHQQGFGS SHELESGQSI TSANHGSHSN QSSCSGTREC
360 370 380 390 400
GSSESSMKKT HVSGSGHSSS TGKYTSTSGQ NYNSTRQGCG QGKSSGSEQY
410 420 430 440 450
GASSGQSSGC SSGQSTRYGE QGSGSRNSST QSRGRSTSRE SSTSQQFGSG
460 470 480 490 500
SGRSSGFSQG GSGQGRSSRG GQQGSFSGQT EGSQQHGSCC GQSSGYGQNE
510 520 530 540 550
YGSGHSASSG QQGSHYSQSS SYGTHNSGGS PSSSQRGHGS RSGRSSGLGQ
560 570 580 590 600
YGSPSGQTSS STRQGSGQGQ ASGSGRYGAS SGQTSGCGSG QSTRYGEQGS
610 620 630 640 650
GSRNSSTQSR GRSTSRESST SQRYGSGSGE SSGFSQGGSG QGRSSRGGQQ
660 670 680 690 700
GSFSGQTSGR SQHQSGSRHG SGSGQFPISG QQGSHHGHSS SSGTHNSGSS
710 720 730 740 750
QSSSTQWSHG SGSEQSSGLG HYGSTSGQTA SSTRQGSGQG QASGSGRCGA
760 770 780 790 800
SSGQTSGCGS GQSTRYGEQG SGSRNSSTQS RGRSTSRESS TSQRFGSGSG
810 820 830 840 850
GSSGFSQGRS GQGRSSRGGQ QGSFSGQTEG SQQHGSCCGQ SSGYGQNEYG
860 870 880 890 900
SGHSASSGQQ GSHYSQSSSY GTHNSGGSPS SRPAGGHGSR SGRSSGLGQY
910 920 930 940 950
GSPSGQTSSS TRQGSGQGQA SGSGRYGASS GQTSGCGSGQ STRYGEQGSG
960 970 980 990 1000
SRNSSTQSRG RSTSRESSTS QRYGSGSGES SGFSQGGSGQ GRSSRGGQQG
1010 1020 1030 1040 1050
SFSGQTSGRS QHQSGSRHGS GSGQFPISGQ QGSHHGHSSS SGTHNSGSSQ
1060 1070 1080 1090 1100
SSSTQWSHGS GSEQSSGLGH YGSTSGQTAS STRQGSGQGQ ASGSGRCGAS
1110 1120 1130 1140 1150
SGQTSGCGSG QSTRYGEQGS GSRNSSTQSR GRSTSRESST SQRFGSGSGG
1160 1170 1180 1190 1200
SSGFSQGRSG QGRSSRGGQQ GSFSGQTEGS QQHGSCCGQS SGYGQNEYGS
1210 1220 1230 1240 1250
GHSASSGQQG SHYSQSSSYG THNSGGSPSS SQRGHGSRSG RSSGLGQYGS
1260 1270 1280 1290 1300
PSGQTSSSTR QGSGQGQASG SGRYGASSGQ TSGCRSGQST RYGGQGSGSR
1310 1320 1330 1340 1350
NSSTQSRGRS TSRESSTSQR YGSGSGESSG FSQGGSGQGR SSRGGQQGSF
1360 1370 1380 1390 1400
SGQTSGRNQH QSGSRHGSGS GQFPISGQQG SHHGHSSSSG THNSGSSQSS
1410 1420 1430 1440 1450
STQWSHGSGS EQSSGLGHYG STSGQTASST RQGSGQGQAS GSGRCGASSG
1460 1470 1480 1490 1500
QTSGCGSGQS TRYGEQGSGS RNSSTQSRGR STSRESSTSQ RFGSGSGGSS
1510 1520 1530 1540 1550
GFSQGRSGQG RSSRGGQQGS FSGQTEGSQQ HGSCCGQSSG YGQNEYGSGH
1560 1570 1580 1590 1600
SASSGQQGSH YSQSSSYGTH NSGGSPSSSQ RGHGSRSGRS SGLGQYGSPS
1610 1620 1630 1640 1650
GQTSSSTRQG SGQGQASGSG RYGASSGQTS GCGSGQPTRY GEQGSGSRNS
1660 1670 1680 1690 1700
STQSRGRSTS RESSTSQRCG SGSGESSGFS QGGSGQGRSS RGGQQGSFSG
1710 1720 1730 1740 1750
QTSGRSQHQS GSRHGSGSGQ FPISGQQGSH HGHSSSSGTH NSGSSQSSST
1760 1770 1780 1790 1800
QWSHGSGSEQ SSGLGHYGST SGQTASSTRQ GSGQGQASGS GRCGASSGQT
1810 1820 1830 1840 1850
SGCGSDQSTR YGEQGSGSRN SSTQSRGRST SRESSTSQRF GSGSGGSSGF
1860 1870 1880 1890 1900
SQGRSGQGRS SRGGQQGSFS GQTEGSQQHG SCCGQSSGYG QNEYGSGHSA
1910 1920 1930 1940 1950
SSGQQGSHYS QSSSYGTHNS GGSPSSSQRG HGSRSGRSSG LGQYGSPSGQ
1960 1970 1980 1990 2000
TSSSTRQGSG QGQASGSGRY GASSGQTSGC GSGQSTRYGE QGSGSRNSST
2010 2020 2030 2040 2050
QSRGRSTSRE SSTSQRYGSG SGESSGFSQG GSGQGRSSRG GQQGSFSGQT
2060 2070 2080 2090 2100
SGRSQHQSGS RHGSGSGQFP ISGQQGSHHG HSSSSGTHNS GSSQSSSTQW
2110 2120 2130 2140 2150
SHGSGSEQSS GLGHYGSTSG QTASSTRQGS GQGQASGSGR CGASSGQTSG
2160 2170 2180 2190 2200
CGSGQSTRYG EQGSGSRNSS TQSRGRSTSR ESSTSQRYGS GSGESSGFSQ
2210 2220 2230 2240 2250
GGSGQGRSSR GGQQGSFSGQ TSGRSQHQSG SRHGSGSGQF PISGQQGSHH
2260 2270 2280 2290 2300
GHSSSSGTHN SGSSQSSSTQ WSHGSGSEQS SGLGQYGSPS GQTSSSTRQG
2310 2320 2330 2340 2350
SGQGQASGSG RYGASSGQTS GCGSGQSTRY GEQGSGSRNS STQSRGRSTS
2360 2370 2380 2390 2400
RESSTSQRYG SGSGESSGFS QGGSGQGRSS RGGQQGSFSG QTSGRSQHQS
2410 2420 2430 2440 2450
GSRHGSGSGQ FPISGQQGSH HGHSSSSGTH NSGSSQSSST QWSHGSGSEQ
2460 2470 2480 2490 2496
SSGLGHYGST SGQTASSTRQ GSGQGQASGS GRCGASSGQT SGCGSG

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

Click a point on a node
to view detailed assay information below
All other points link out to UniProt


Phosphorylation Acetylation Ubiquitylation Other

loading

Loader

Assay Details for non-CPTAC-4039 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hrnr
Peptide Sequence
ELLVTEFHQILK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
35
Peptide End
46
CPTAC ID
non-CPTAC-4039
Peptide Molecular Mass
1,468.8290
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (2+) 13.2 3 1.8 15.8 5.1 2.8 20.6 5.9 3.3 15 15 15
y9 (2+) 10.5 2.6 1.5 12.5 4.7 2.4 16.3 5.4 2.8 15 15 15
y8 (2+) 4.7 1.6 1.2 7.4 4.7 3.6 8.8 5 3.8 15 15 15
y10 (3+) 10.7 2.7 1.5 12.8 5.6 2.9 16.7 6.2 3.3 15 15 15
b8 (2+) 59.7 61.1 53.9 60 63 61 84.6 87.8 81.4 15 15 15
sum 2.5 0.7 0.6 6 4.5 3 6.5 4.6 3.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments