Hmgn5, CPTAC-4040 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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Overview Data source: UniProt

Official Gene Symbol	Other Aliases
Hmgn5	Garp45, Nsbp1

Sequence Length (AA)	Molecular Weight (Da)
406	45344

Protein Name
High mobility group nucleosome-binding domain-containing protein 5

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MPKRKAAGDV	SQEPKRRSAR	LSAMPVPFTP	ELKPKRASTS	RKTKTTNVVE
60	70	80	90	100
ENKDASTIPI	PETKPEDVKD	ECNMENAENG	EAKIMEAPIP	KMEAEEVKEQ
110	120	130	140	150
INEDTEEDGG	EKKEAVAAEA	KDDELKANIQ	DVEKDEDGKE	HKDTGEEVED
160	170	180	190	200
GKIEEEGLNE	KPGTAKSEDA	EVSKDEEEKG	DNEKGEDGKE	EGDEKEEEKD
210	220	230	240	250
DKEGDTGTEK	EVKEQNKEAE	EDDGKCKEEE	NKEVGKEGQP	EEDGKEDLHE
260	270	280	290	300
EVGKEDLHEE	DGKEGQPEED	GKEIHHEEDG	KEGQPEEDGK	EYLHEEDGEE
310	320	330	340	350
GQPKEDQKEG	QPEEDGKEDQ	PEEDGKEGQC	KEDGKEGHHE	EGGKEDLHEE
360	370	380	390	400
DGKEKDGGKE	DRKEEGEQEV	AVDEGSDENK	VEAEEEGAEN	KDFKQDGEKE
406
EPLSIV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-4040 Collapse assay details

Data source: Panorama

Official Gene Symbol: Hmgn5
Peptide Sequence: AAGDVSQEPK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 6
Peptide End: 15

CPTAC ID: non-CPTAC-4040
Peptide Molecular Mass: 1,000.4825
Species: Mus musculus (Mouse)
Assay Type: Direct MRM
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b7 (2+)	6.2	5.2	4.1	8.2	7.8	9	10.3	9.4	9.9	15	15	15
y9 (2+)	13.3	9.6	11.8	14.5	15.3	13.8	19.7	18.1	18.2	15	15	15
y5 (1+)	6.9	2.5	4.2	7.1	8.1	10.3	9.9	8.5	11.1	15	15	15
y6 (1+)	4	2.6	3.5	5.4	6.9	10	6.7	7.4	10.6	15	15	15
y8 (1+)	4.2	2.6	2.5	6.9	8	10.5	8.1	8.4	10.8	15	15	15
sum	3.9	2.4	2.4	5.2	6.9	9.6	6.5	7.3	9.9	15	15	15

Hmgn5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-4040 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

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Hmgn5

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-4040 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments