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Overview Data source: UniProt

Official Gene Symbol Other Aliases
Hmgn5 Garp45, Nsbp1
Sequence Length (AA) Molecular Weight (Da)
406 45344
Protein Name
High mobility group nucleosome-binding domain-containing protein 5
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MPKRKAAGDV SQEPKRRSAR LSAMPVPFTP ELKPKRASTS RKTKTTNVVE
60 70 80 90 100
ENKDASTIPI PETKPEDVKD ECNMENAENG EAKIMEAPIP KMEAEEVKEQ
110 120 130 140 150
INEDTEEDGG EKKEAVAAEA KDDELKANIQ DVEKDEDGKE HKDTGEEVED
160 170 180 190 200
GKIEEEGLNE KPGTAKSEDA EVSKDEEEKG DNEKGEDGKE EGDEKEEEKD
210 220 230 240 250
DKEGDTGTEK EVKEQNKEAE EDDGKCKEEE NKEVGKEGQP EEDGKEDLHE
260 270 280 290 300
EVGKEDLHEE DGKEGQPEED GKEIHHEEDG KEGQPEEDGK EYLHEEDGEE
310 320 330 340 350
GQPKEDQKEG QPEEDGKEDQ PEEDGKEGQC KEDGKEGHHE EGGKEDLHEE
360 370 380 390 400
DGKEKDGGKE DRKEEGEQEV AVDEGSDENK VEAEEEGAEN KDFKQDGEKE
406
EPLSIV

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for non-CPTAC-4040 Collapse assay details

Data source: Panorama

Official Gene Symbol
Hmgn5
Peptide Sequence
AAGDVSQEPK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
6
Peptide End
15
CPTAC ID
non-CPTAC-4040
Peptide Molecular Mass
1,000.4825
Species
Mus musculus (Mouse)
Assay Type
Direct MRM
Matrix
Plasma
Submitting Laboratory
UVic-Genome BC Proteomics Centre
Submitting Lab PI
Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Agilent 6490/6495 QQQ
Internal Standard
synthetic peptide
Peptide Standard Purity
>80%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
1290 LC (Agilent)
Column Packing
Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions
2.1 x 150 mm
Flow Rate
400 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b7 (2+) 6.2 5.2 4.1 8.2 7.8 9 10.3 9.4 9.9 15 15 15
y9 (2+) 13.3 9.6 11.8 14.5 15.3 13.8 19.7 18.1 18.2 15 15 15
y5 (1+) 6.9 2.5 4.2 7.1 8.1 10.3 9.9 8.5 11.1 15 15 15
y6 (1+) 4 2.6 3.5 5.4 6.9 10 6.7 7.4 10.6 15 15 15
y8 (1+) 4.2 2.6 2.5 6.9 8 10.5 8.1 8.4 10.8 15 15 15
sum 3.9 2.4 2.4 5.2 6.9 9.6 6.5 7.3 9.9 15 15 15


Additional Resources and Comments