ALB, CPTAC-6079 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
ALB	N/A

Sequence Length (AA)	Molecular Weight (Da)
609	69367

Protein Name
Albumin

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MKWVTFISLL	FLFSSAYSRG	VFRRDAHKSE	VAHRFKDLGE	ENFKALVLIA
60	70	80	90	100
FAQYLQQCPF	EDHVKLVNEV	TEFAKTCVAD	ESAENCDKSL	HTLFGDKLCT
110	120	130	140	150
VATLRETYGE	MADCCAKQEP	ERNECFLQHK	DDNPNLPRLV	RPEVDVMCTA
160	170	180	190	200
FHDNEETFLK	KYLYEIARRH	PYFYAPELLF	FAKRYKAAFT	ECCQAADKAA
210	220	230	240	250
CLLPKLDELR	DEGKASSAKQ	RLKCASLQKF	GERAFKAWAV	ARLSQRFPKA
260	270	280	290	300
EFAEVSKLVT	DLTKVHTECC	HGDLLECADD	RADLAKYICE	NQDSISSKLK
310	320	330	340	350
ECCEKPLLEK	SHCIAEVEND	EMPADLPSLA	ADFVESKDVC	KNYAEAKDVF
360	370	380	390	400
LGMFLYEYAR	RHPDYSVVLL	LRLAKTYETT	LEKCCAAADP	HECYAKVFDE
410	420	430	440	450
FKPLVEEPQN	LIKQNCELFE	QLGEYKFQNA	LLVRYTKKVP	QVSTPTLVEV
460	470	480	490	500
SRNLGKVGSK	CCKHPEAKRM	PCAEDYLSVV	LNQLCVLHEK	TPVSDRVTKC
510	520	530	540	550
CTESLVNRRP	CFSALEVDET	YVPKEFNAET	FTFHADICTL	SEKERQIKKQ
560	570	580	590	600
TALVELVKHK	PKATKEQLKA	VMDDFAAFVE	KCCKADDKET	CFAEEGKKLV
609
AASQAALGL

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for non-CPTAC-6079 Collapse assay details

Data source: Panorama

Official Gene Symbol: ALB
Peptide Sequence: LVNEVTEFAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 66
Peptide End: 75

CPTAC ID: non-CPTAC-6079
Peptide Molecular Mass: 1,148.6077
Species: Homo sapiens (Human)
Assay Type: Direct MRM-MS
Matrix: Bovine Serum Albumin
Submitting Laboratory: McGill University
Submitting Lab PI: Christoph H. Borchers, Gerald Batist

Publication

View Details (opens in a new window)

mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6495B QQQ-MS
Internal Standard: Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8)
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Infinity 1290
Column Packing: commercial (Agilent ZORBAX Eclipse Plus C18)
Column Dimensions: 4.6 mm × 150 mm, (5 µm)
Flow Rate: 0.4 mL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	9.5	8	8.3	9.4	7.9	5.7	13.4	11.2	10.1	16	16	17
y5 (1+)	16.1	9.6	11.9	15.7	9.3	6.6	22.5	13.4	13.6	16	16	17
y6 (1+)	9.4	6.4	7.7	9.4	8.3	5.9	13.3	10.5	9.7	16	16	17
y7 (1+)	11.2	7.2	9.2	10.7	8.6	7.8	15.5	11.2	12.1	16	16	17
y8 (1+)	12.9	7.6	7.7	12.6	6.9	7.1	18	10.3	10.5	16	16	17
sum	5.2	5.2	3.9	5.9	4.6	3.9	7.9	6.9	5.5	16	16	17

Stability

Data source: Panorama

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Fragment ion / Transition	control_intra_CV	actual_temp_intra_CV	frozen_intra_CV	FTx1_intra_CV	FTx2_intra_CV
b2 (1+)	2.2	7.8	2.2	5.3	5.1
y5 (1+)	8	4.6	5.7	5	6.5
y6 (1+)	8.4	7.7	11.4	14.7	4.7
y7 (1+)	8.3	5	9.5	13.1	6.4
y8 (1+)	8	4.1	6.4	8.3	3.8
sum	4.6	3.4	3	4.1	0.4

Fragment ion / Transition	all_intra_CV	all_inter_CV
b2 (1+)	5.1	4.7
y5 (1+)	5.7	6.4
y6 (1+)	9.1	8.8
y7 (1+)	7.9	7.6
y8 (1+)	5.8	6.4
sum	3.2	3.5

Endogenous

Data source: Panorama

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Fragment ion / Transition	intra_CV	inter_CV	total_CV
b2 (1+)	18.1	26.6	32.2
y5 (1+)	42.6	12.2	44.3
y6 (1+)	68.4	41.9	80.2
y7 (1+)	5.2	81	81.2
y8 (1+)	19.6	31.7	37.3
sum	13.9	17	22

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-1160 Collapse assay details

Data source: Panorama

Official Gene Symbol: ALB
Peptide Sequence: LVNEVTEFAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 66
Peptide End: 75

CPTAC ID: non-CPTAC-1160
Peptide Molecular Mass: 1,148.6077
Species: Homo sapiens (Human)
Assay Type: Direct MRM-MS
Matrix: plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: 6490 Triple Quad (Agilent)
Internal Standard: synthetic peptide
Peptide Standard Purity: >95%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	7	3.4	3.7	15.6	10.4	3.6	17.1	10.9	5.2	15	14	13
y2 (1+)	5.3	4.7	4.4	9.2	8	7.5	10.6	9.3	8.7	15	15	15
y3 (1+)	6.4	5.5	2.7	11.7	7.5	3.3	13.3	9.3	4.3	15	15	14
y6 (1+)	3.6	3.2	3	8.8	5.2	5.3	9.5	6.1	6.1	15	15	14
y8 (1+)	4.5	3.7	3.5	12.3	7.6	4	13.1	8.5	5.3	15	15	14
sum	2.5	1.8	2.7	11.9	7.2	3.8	12.2	7.4	4.7	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for non-CPTAC-3890 Collapse assay details

Data source: Panorama

Official Gene Symbol: Alb
Peptide Sequence: LVQEVTDFAK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 66
Peptide End: 75

CPTAC ID: non-CPTAC-3890
Peptide Molecular Mass: 1,148.6077
Species: Mus musculus (Mouse)
Assay Type: Direct MRM-MS
Matrix: Plasma
Submitting Laboratory: UVic-Genome BC Proteomics Centre
Submitting Lab PI: Christoph Borchers

Publication

View Details (opens in a new window)

Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: Agilent 6490/6495 QQQ
Internal Standard: synthetic peptide
Peptide Standard Purity: >80%
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: 1290 LC (Agilent)
Column Packing: Zorbax Eclipse Plus C18, 1.8 µm
Column Dimensions: 2.1 x 150 mm
Flow Rate: 400 µL/min

Chromatograms

Data source: Panorama

view larger image

Response Curves

Data source: Panorama

view larger image

Retrieving Data

Repeatability

Data source: Panorama

view larger image

	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
b2 (1+)	4.4	4.2	2.7	10.2	11.8	11.4	11.1	12.5	11.7	15	15	15
y8 (2+)	4.2	2.9	2.1	10.5	13.9	9	11.3	14.2	9.2	15	15	15
y3 (1+)	5.6	2.8	3.9	12.3	15.7	17.2	13.5	15.9	17.6	15	15	15
y7 (1+)	3.9	4.4	4.9	12	13.7	14.6	12.6	14.4	15.4	15	15	15
y8 (1+)	3.4	3.7	1.7	10.5	13	11.9	11	13.5	12	15	15	15
sum	2.8	1.3	1.9	9.8	12.8	10.9	10.2	12.9	11.1	15	15	15

Search form

ALB

Overview Data source: UniProt

Protein Sequence hover to view complete sequence

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Assay Details for non-CPTAC-6079 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Stability

Endogenous

Additional Resources and Comments

Comments

Assay Details for non-CPTAC-1160 Collapse assay details

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments

Assay Details for non-CPTAC-3890 Collapse assay details

Publication

Assay Parameters Collapse assay parameters

Chromatograms

Response Curves

Repeatability

Additional Resources and Comments

Comments