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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

EPHA7

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
EPHA7 EHK3, HEK11
Sequence Length (AA) Molecular Weight (Da)
998 112097
Protein Name
Ephrin type-A receptor 7
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MVFQTRYPSW IILCYIWLLR FAHTGEAQAA KEVLLLDSKA QQTELEWISS
60 70 80 90 100
PPNGWEEISG LDENYTPIRT YQVCQVMEPN QNNWLRTNWI SKGNAQRIFV
110 120 130 140 150
ELKFTLRDCN SLPGVLGTCK ETFNLYYYET DYDTGRNIRE NLYVKIDTIA
160 170 180 190 200
ADESFTQGDL GERKMKLNTE VREIGPLSKK GFYLAFQDVG ACIALVSVKV
210 220 230 240 250
YYKKCWSIIE NLAIFPDTVT GSEFSSLVEV RGTCVSSAEE EAENAPRMHC
260 270 280 290 300
SAEGEWLVPI GKCICKAGYQ QKGDTCEPCG RGFYKSSSQD LQCSRCPTHS
310 320 330 340 350
FSDKEGSSRC ECEDGYYRAP SDPPYVACTR PPSAPQNLIF NINQTTVSLE
360 370 380 390 400
WSPPADNGGR NDVTYRILCK RCSWEQGECV PCGSNIGYMP QQTGLEDNYV
410 420 430 440 450
TVMDLLAHAN YTFEVEAVNG VSDLSRSQRL FAAVSITTGQ AAPSQVSGVM
460 470 480 490 500
KERVLQRSVE LSWQEPEHPN GVITEYEIKY YEKDQRERTY STVKTKSTSA
510 520 530 540 550
SINNLKPGTV YVFQIRAFTA AGYGNYSPRL DVATLEEATG KMFEATAVSS
560 570 580 590 600
EQNPVIIIAV VAVAGTIILV FMVFGFIIGR RHCGYSKADQ EGDEELYFHF
610 620 630 640 650
KFPGTKTYID PETYEDPNRA VHQFAKELDA SCIKIERVIG AGEFGEVCSG
660 670 680 690 700
RLKLPGKRDV AVAIKTLKVG YTEKQRRDFL CEASIMGQFD HPNVVHLEGV
710 720 730 740 750
VTRGKPVMIV IEFMENGALD AFLRKHDGQF TVIQLVGMLR GIAAGMRYLA
760 770 780 790 800
DMGYVHRDLA ARNILVNSNL VCKVSDFGLS RVIEDDPEAV YTTTGGKIPV
810 820 830 840 850
RWTAPEAIQY RKFTSASDVW SYGIVMWEVM SYGERPYWDM SNQDVIKAIE
860 870 880 890 900
EGYRLPAPMD CPAGLHQLML DCWQKERAER PKFEQIVGIL DKMIRNPNSL
910 920 930 940 950
KTPLGTCSRP ISPLLDQNTP DFTTFCSVGE WLQAIKMERY KDNFTAAGYN
960 970 980 990 998
SLESVARMTI EDVMSLGITL VGHQKKIMSS IQTMRAQMLH LHGTGIQV

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2787 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPHA7
Peptide Modified Sequence
TYIDPETY[+80.0]EDPNR
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
8
Peptide Start
607
Peptide End
619
CPTAC ID
CPTAC-2787
Peptide Molecular Mass
1,691.6716
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Mammalian Cell Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
250nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 4.6 4.1 5 6.7 4.5 4.1 8.1 6.1 6.5 15 15 15
y9 (1+) 5.2 3 4.5 7.2 4.7 3.9 8.9 5.6 6 15 15 15
y7 (1+) 7.1 4.7 2.3 8.5 5.3 2.1 11.1 7.1 3.1 15 15 15
y3 (1+) 4.1 2.8 3.5 6.6 4.6 3.5 7.8 5.4 4.9 15 15 15
b2 (1+) 6.1 3.1 3.8 7.8 4.4 3.1 9.9 5.4 4.9 15 15 15
sum 5 2.7 3.8 7 4.2 3.3 8.6 5 5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
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Comments

Assay Details for CPTAC-1783 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPHA7
Peptide Modified Sequence
WTAPEAIQY[+80.0]R
Modification Type
Phospho (Y)
Protein - Site of Modification
810
Peptide - Site of Modification
9
Peptide Start
802
Peptide End
811
CPTAC ID
CPTAC-1783
Peptide Molecular Mass
1,313.5806
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y4 (1+) 141.4 18.2 17.1 141.4 17.8 17.2 200 25.5 24.3 7 13 14
y5 (1+) 147.8 7.6 5.4 123.3 12.8 7.1 192.5 14.9 8.9 11 15 15
y7 (1+) 29.8 7.8 5.9 24.3 8.1 6.3 38.5 11.2 8.6 15 15 15
y8 (1+) 103.3 12.4 8 146.6 16.6 13.8 179.3 20.7 16 12 15 15
sum 47.8 5.4 3.5 43.9 6.6 6.5 64.9 8.5 7.4 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-2830 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPHA7
Peptide Sequence
FEQIVGILDK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
883
Peptide End
892
CPTAC ID
CPTAC-2830
Peptide Molecular Mass
1,160.6441
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive™
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
EASY-nLC™ 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

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view larger image
view larger image

Response Curves

Data source: Panorama

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view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y6 (1+) 11.8 9.3 6.2 10.6 10.5 7.7 15.9 14 9.9 15 15 15
y5 (1+) 12.3 10.5 7.2 11.9 11.1 7.5 17.1 15.3 10.4 15 15 15
y3 (1+) 11.7 10.5 8.8 15.4 10.6 9.2 19.3 14.9 12.7 15 15 15
sum 10.8 9.9 7.1 11.4 10.5 7.8 15.7 14.4 10.5 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments