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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

EPHA2

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Available Assays

Overview Data source: UniProt

Official Gene Symbol Other Aliases
EPHA2 ECK
Sequence Length (AA) Molecular Weight (Da)
976 108266
Protein Name
Ephrin type-A receptor 2
Sources
UniProt
PhosphoSitePlus ®
GeneCards 		Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MELQAARACF ALLWGCALAA AAAAQGKEVV LLDFAAAGGE LGWLTHPYGK
60 70 80 90 100
GWDLMQNIMN DMPIYMYSVC NVMSGDQDNW LRTNWVYRGE AERIFIELKF
110 120 130 140 150
TVRDCNSFPG GASSCKETFN LYYAESDLDY GTNFQKRLFT KIDTIAPDEI
160 170 180 190 200
TVSSDFEARH VKLNVEERSV GPLTRKGFYL AFQDIGACVA LLSVRVYYKK
210 220 230 240 250
CPELLQGLAH FPETIAGSDA PSLATVAGTC VDHAVVPPGG EEPRMHCAVD
260 270 280 290 300
GEWLVPIGQC LCQAGYEKVE DACQACSPGF FKFEASESPC LECPEHTLPS
310 320 330 340 350
PEGATSCECE EGFFRAPQDP ASMPCTRPPS APHYLTAVGM GAKVELRWTP
360 370 380 390 400
PQDSGGREDI VYSVTCEQCW PESGECGPCE ASVRYSEPPH GLTRTSVTVS
410 420 430 440 450
DLEPHMNYTF TVEARNGVSG LVTSRSFRTA SVSINQTEPP KVRLEGRSTT
460 470 480 490 500
SLSVSWSIPP PQQSRVWKYE VTYRKKGDSN SYNVRRTEGF SVTLDDLAPD
510 520 530 540 550
TTYLVQVQAL TQEGQGAGSK VHEFQTLSPE GSGNLAVIGG VAVGVVLLLV
560 570 580 590 600
LAGVGFFIHR RRKNQRARQS PEDVYFSKSE QLKPLKTYVD PHTYEDPNQA
610 620 630 640 650
VLKFTTEIHP SCVTRQKVIG AGEFGEVYKG MLKTSSGKKE VPVAIKTLKA
660 670 680 690 700
GYTEKQRVDF LGEAGIMGQF SHHNIIRLEG VISKYKPMMI ITEYMENGAL
710 720 730 740 750
DKFLREKDGE FSVLQLVGML RGIAAGMKYL ANMNYVHRDL AARNILVNSN
760 770 780 790 800
LVCKVSDFGL SRVLEDDPEA TYTTSGGKIP IRWTAPEAIS YRKFTSASDV
810 820 830 840 850
WSFGIVMWEV MTYGERPYWE LSNHEVMKAI NDGFRLPTPM DCPSAIYQLM
860 870 880 890 900
MQCWQQERAR RPKFADIVSI LDKLIRAPDS LKTLADFDPR VSIRLPSTSG
910 920 930 940 950
SEGVPFRTVS EWLESIKMQQ YTEHFMAAGY TAIEKVVQMT NDDIKRIGVR
960 970 976
LPGHQKRIAY SLLGLKDQVN TVGIPI

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-2783 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPHA2
Peptide Modified Sequence
QSPEDVY[+80.0]FSK
Modification Type
Phospho (Y)
Protein - Site of Modification
N/A
Peptide - Site of Modification
7
Peptide Start
569
Peptide End
578
CPTAC ID
CPTAC-2783
Peptide Molecular Mass
1,278.5170
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
Mammalian Cell Lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
250nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

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  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y8 (1+) 9.3 8.1 8.7 15.1 8.2 7.3 17.7 11.5 11.4 15 15 15
y6 (1+) 7.7 7.6 8.6 14.8 7.7 8.1 16.7 10.8 11.8 15 15 15
y5 (1+) 9 8.3 9.9 15.2 8.4 8.3 17.7 11.8 12.9 15 15 15
y4 (1+) 9.3 8.1 8.1 15.3 8.3 6.7 17.9 11.6 10.5 15 15 15
y3 (1+) 9.4 8.8 8.7 15.6 8.7 7.4 18.2 12.4 11.4 15 15 15
sum 8.8 8 8.6 15.1 8.1 7.3 17.5 11.4 11.3 15 15 15

Additional Resources and Comments

Resources
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Comments

Assay Details for CPTAC-1784 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPHA2
Peptide Modified Sequence
VIGAGEFGEVY[+80.0]K
Modification Type
Phospho (Y)
Protein - Site of Modification
628
Peptide - Site of Modification
11
Peptide Start
618
Peptide End
629
CPTAC ID
CPTAC-1784
Peptide Molecular Mass
1,347.6112
Species
Homo sapiens (Human)
Assay Type
Direct PRM
Matrix
E.coli lysate
Submitting Laboratory
Johns Hopkins University / Pandey
Submitting Lab PI
Akhilesh Pandey

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
Orbitrap Fusion Lumos
Internal Standard
100fmol
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Easy nLC 1200 System
Column Packing
C18 2um 100A
Column Dimensions
75um i.d. x 50cm
Flow Rate
300 nl/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

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Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y10 (1+) 13.9 4.2 4.3 18.8 7.6 5 23.4 8.7 6.6 15 15 15
y8 (1+) 6.6 6.5 4.2 10 8 6.9 12 10.3 8.1 15 15 15
y6 (1+) 25.8 3.5 5.5 28.9 8.9 6.4 38.7 9.6 8.4 15 15 15
y2 (1+) 25.6 9.1 6.2 32.3 11 6.7 41.2 14.3 9.1 15 15 15
sum 11.8 4.5 3.6 17 7.6 4.9 20.7 8.8 6.1 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments

Assay Details for CPTAC-3201 Collapse assay details

Data source: Panorama

Official Gene Symbol
EPHA2
Peptide Sequence
IDTIAPDEITVSSDFEAR
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
142
Peptide End
159
CPTAC ID
CPTAC-3201
Peptide Molecular Mass
1,977.9531
Species
Homo Sapiens
Assay Type
Direct PRM
Matrix
Tumor Digest
Submitting Laboratory
Washington University in St. Louis
Submitting Lab PI
Reid Townsend

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
ThermoFisher, Q-Exactive
Internal Standard
25 fmol on column
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus R
LC
Easy-nLC 1000 Thermo Scientific
Column Packing
C18
Column Dimensions
75 µm x 50 cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama

view larger image
view larger image
view larger image

Response Curves

Data source: Panorama

view larger image
view larger image
view larger image

Retrieving Data

Loader

Repeatability

Data source: Panorama

view larger image
  Average intra-assay CV
(within day CV)		 Average inter-assay CV
(between day CV)		 Total CV
equation		 n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 9.8 3.3 3.7 9.3 5.8 3.2 13.5 6.7 4.9 15 15 15
b3 (1+) 5.9 2.4 2.4 8.3 4.2 2.4 10.2 4.8 3.4 15 15 15
y13 (1+) 7.4 3 2.7 9 5.8 4.2 11.7 6.5 5 15 15 15
sum 6.6 2.6 2.3 8.2 5.2 3.2 10.5 5.8 3.9 15 15 15

Additional Resources and Comments

Resources
Download SOPs
View Peptide in Panorama
View Protein in Panorama
Comments