CPA3, CPTAC-3270 - CPTAC Assay Portal | Office of Cancer Clinical Proteomics Research

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We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol	Other Aliases
CPA3	N/A

Sequence Length (AA)	Molecular Weight (Da)
417	48673

Protein Name
Uncharacterized protein

Sources
UniProt PhosphoSitePlus ® GeneCards	Human Protein Atlas

Protein Sequence hover to view complete sequence

10	20	30	40	50
MWFILPVGLI	ATTLAIAPVR	FDREKVFRVK	PQDEKQANII	KDLAKTNQLD
60	70	80	90	100
FWYPDATHHV	TANMTIDFQV	SEKESQSIQS	ALDQNNMHYE	ILIHDLQEEI
110	120	130	140	150
EKQFDVKEDI	PGRHSYAKYN	NWDKIVAWTE	KMVHKHPDMV	SRIKIGTTVE
160	170	180	190	200
DNPLYVLKVG	KGDERRKAIF	MDCGIHAREW	ISPAFCQWFV	YQATKTYGKN
210	220	230	240	250
KIMTKLLDRM	NFYVLPVFNV	DGYIWSWTQN	RMWRKNRSKN	QNSKCIGTDL
260	270	280	290	300
NRNFNASWNS	FQNTKNPCAN	IYRGPKPESE	KETKAVTDFI	RSHLKSIKAY
310	320	330	340	350
ITFHSYSQML	LFPYGYTSKL	PPNHEDLDKV	AKISTDVLAT	RYETRYIYGP
360	370	380	390	400
IASTYYPTSG	SSLDWAYNVG	IKHTFAFELR	DKGKYGFLLP	ESLIKSTCKE
410	417
TLLAVKFIAK	YILKHTS

Data source: UniProt

Protein Map Collapse protein map

Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-3270 Collapse assay details

Data source: Panorama

Official Gene Symbol: CPA3
Peptide Sequence: NFNASWNSFQNTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 253
Peptide End: 265

CPTAC ID: CPTAC-3270
Peptide Molecular Mass: 1,556.7008
Species: Canis lupus familiaris (Dog)
Assay Type: Direct MRM
Matrix: Serum
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument: TSQ Vantage
Internal Standard: Isotope labeled peptide
Peptide Standard Purity: Crude (~60%)
Peptide Standard Label Type: 13C and 15N at C-terminus K

LC: Accela 1250 Quaternary Low pump
Column Packing: C18 3 µm 300 Å
Column Dimensions: 1.0 mm I.D. x 15 cm
Flow Rate: 50 µL/min

Chromatograms

Data source: Panorama

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Response Curves

Data source: Panorama

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Retrieving Data

Repeatability

Data source: Panorama

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	Average intra-assay CV (within day CV)			Average inter-assay CV (between day CV)			Total CV			n=
Fragment ion / Transition	Low	Med	High	Low	Med	High	Low	Med	High	Low	Med	High
y9 (1+)	12.1	7.7	8.1	24.6	17.5	20.9	27.4	19.1	22.4	15	15	15
y8 (1+)	16.5	7	4.9	21.8	19.1	23.2	27.3	20.3	23.7	15	15	15
y7 (1+)	21.3	7.1	6.8	32.9	18.6	22.7	39.2	19.9	23.7	15	15	15
sum	10.5	5.2	3	23.3	16.8	20.8	25.6	17.6	21	15	15	15

Additional Resources and Comments

Resources

Download SOPs

View Peptide in Panorama

View Protein in Panorama

Comments

Assay Details for CPTAC-3275 Collapse assay details

Data source: Panorama

Official Gene Symbol: CPA3
Peptide Sequence: NFNASWNSFQNTK
Modification Type: unmodified
Protein - Site of Modification: N/A
Peptide - Site of Modification: N/A
Peptide Start: 253
Peptide End: 265

CPTAC ID: CPTAC-3275
Peptide Molecular Mass: 1,556.7008
Species: Canis lupus familiaris (Dog)
Assay Type: Direct MRM
Matrix: Tissue
Submitting Laboratory: Johns Hopkins University
Submitting Lab PI: Daniel Chan, Hui Zhang, Zhen Zhang