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Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.

Overview Data source: UniProt

Official Gene Symbol Other Aliases
CPA3 N/A
Sequence Length (AA) Molecular Weight (Da)
417 48673
Protein Name
Uncharacterized protein
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MWFILPVGLI ATTLAIAPVR FDREKVFRVK PQDEKQANII KDLAKTNQLD
60 70 80 90 100
FWYPDATHHV TANMTIDFQV SEKESQSIQS ALDQNNMHYE ILIHDLQEEI
110 120 130 140 150
EKQFDVKEDI PGRHSYAKYN NWDKIVAWTE KMVHKHPDMV SRIKIGTTVE
160 170 180 190 200
DNPLYVLKVG KGDERRKAIF MDCGIHAREW ISPAFCQWFV YQATKTYGKN
210 220 230 240 250
KIMTKLLDRM NFYVLPVFNV DGYIWSWTQN RMWRKNRSKN QNSKCIGTDL
260 270 280 290 300
NRNFNASWNS FQNTKNPCAN IYRGPKPESE KETKAVTDFI RSHLKSIKAY
310 320 330 340 350
ITFHSYSQML LFPYGYTSKL PPNHEDLDKV AKISTDVLAT RYETRYIYGP
360 370 380 390 400
IASTYYPTSG SSLDWAYNVG IKHTFAFELR DKGKYGFLLP ESLIKSTCKE
410 417
TLLAVKFIAK YILKHTS

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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to view detailed assay information below
All other points link out to UniProt



Phosphorylation Acetylation Ubiquitylation Other

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Assay Details for CPTAC-3270 Collapse assay details

Data source: Panorama

Official Gene Symbol
CPA3
Peptide Sequence
NFNASWNSFQNTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
253
Peptide End
265
CPTAC ID
CPTAC-3270
Peptide Molecular Mass
1,556.7008
Species
Canis lupus familiaris (Dog)
Assay Type
Direct MRM
Matrix
Serum
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Isotope labeled peptide
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low pump
Column Packing
C18 3 µm 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 12.1 7.7 8.1 24.6 17.5 20.9 27.4 19.1 22.4 15 15 15
y8 (1+) 16.5 7 4.9 21.8 19.1 23.2 27.3 20.3 23.7 15 15 15
y7 (1+) 21.3 7.1 6.8 32.9 18.6 22.7 39.2 19.9 23.7 15 15 15
sum 10.5 5.2 3 23.3 16.8 20.8 25.6 17.6 21 15 15 15


Additional Resources and Comments


Assay Details for CPTAC-3275 Collapse assay details

Data source: Panorama

Official Gene Symbol
CPA3
Peptide Sequence
NFNASWNSFQNTK
Modification Type
unmodified
Protein - Site of Modification
N/A
Peptide - Site of Modification
N/A
Peptide Start
253
Peptide End
265
CPTAC ID
CPTAC-3275
Peptide Molecular Mass
1,556.7008
Species
Canis lupus familiaris (Dog)
Assay Type
Direct MRM
Matrix
Tissue
Submitting Laboratory
Johns Hopkins University
Submitting Lab PI
Daniel Chan, Hui Zhang, Zhen Zhang

Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
TSQ Vantage
Internal Standard
Isotope labeled peptide
Peptide Standard Purity
Crude (~60%)
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
Accela 1250 Quaternary Low Pump
Column Packing
C18 3 µm 300 Å
Column Dimensions
1.0 mm I.D. x 15 cm
Flow Rate
50 µL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

Loader

Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
y9 (1+) 12.3 7.9 5.5 13.9 10.4 6.4 18.6 13.1 8.4 15 15 15
y8 (1+) 16.8 9.1 7.3 17.6 10.3 8.4 24.3 13.7 11.1 15 15 15
y7 (1+) 17.7 8.1 5.9 22.9 9.1 6.9 28.9 12.2 9.1 15 15 15
sum 7.1 4.9 3.5 8.5 7.1 3.7 11.1 8.6 5.1 15 15 15


Additional Resources and Comments